02002nas a2200253   4500008004100000022001400041245013400055210006900189260007300258300001200331490000700343520106300350653001401413653002501427653001901452653001701471653002301488653002301511100002301534700002001557700001901577700003001596856012201626       2014                        eng d  a0928-493100aIn situ synthesized BSA capped gold nanoparticles: effective carrier of anticancer drug methotrexate to MCF-7 breast cancer cells0 aIn situ synthesized BSA capped gold nanoparticles effective carr  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJAN  a158-1670 v343 a<p>The proficiency of MTX loaded BSA capped gold nanoparticles (Au-BSA-MTX) in inhibiting the proliferation of breast cancer cells MCF-7 as compared to the free drug Methotrexate (MTX) is demonstrated based on MTT and Ki-67 proliferation assays. In addition, DNA ladder gel electrophoresis studies, flow cytometry and TUNEL assay confirmed the induction of apoptosis by MTX and Au-BSA-MTX in MCF-7 cells. Notably, Au-BSA-MTX: was found to have higher cytotoxicity on MCF-7 cells compared with an equivalent dose of free MTX. The enhanced activity is attributed to the preferential uptake of Au-BSA-MTX particles by MCF-7 cells due to the presence of BSA that acts as a source of nutrient and energy to the rapidly proliferating MCF-7 cells. Moreover, the targeting ability of the drug MTX to the over expressed folate receptors on MCF-7 cells also contributes to the enhanced uptake and activity. Taken together, these results unveil that Au-BSA-MTX could be more effective than free drug for cancer treatment. (C) 2013 Elsevier B.V. All rights reserved.</p>10aApoptosis10aBovine serum albumin10aCancer therapy10aCytotoxicity10aDual active agents10agold nanoparticles1 aMurawala, Priyanka1 aTirmale, Amruta1 aShiras, Anjali1 aPrasad, Bhagavatula L. V.  uhttps://library.ncl.res.in/content/situ-synthesized-bsa-capped-gold-nanoparticles-effective-carrier-anticancer-drug-0