02137nas a2200193   4500008004100000245013500041210006900176260000800245490000700253520142000260100002501680700001701705700001801722700001901740700001601759700001801775700001901793856013101812       2018                        eng d00aEvaluation of Candida tropicalis (NCIM 3321) extracellular phytase having plant growth promoting potential and process development0 aEvaluation of Candida tropicalis NCIM 3321 extracellular phytase  cJAN0 v133 a<p>Phytase is known to provide a solution for depletion of phosphorus (P). It helps it by hydrolyzing the insoluble P source in soil which is phytate. In this study, provides insight on yeast Candida tropicalis (NCIM 3321) which produces cell bound and extracellular thermostable phytase. The media components were optimized to enhance the enzyme production and checked for plant growth promoting activity. On optimization the isolate exhibited enhanced cell bound and extracellular phytase activity by four folds (from 236 to 1024 IU DCG⁻¹) and by five folds (from 0.46 to 1.95 IU ml⁻¹) respectively in 36 h. The production time decreased to 24 h compare to shake flask on Up-scaling the production process upto 10 L scale, thus increasing the productivity of cell bound (1810 IU DCG⁻¹day⁻¹) and extracellular phytase (6.08 IU ml⁻¹ day⁻¹). The crude phytase (12 IU) from NCIM 3321 strain was studied for plant growth promotion activity in lab scale and field level experiments with maize crop. Findings of the study revealed that the extracellular phytase derived from non pathogenic C. tropicalis (NCIM 3321) was found to be plant growth stimulating by increasing the available P in soil. Our findings of phytase isolated from non-pathogenic yeast C. tropicalis NCIM 3321 exhibited dephytinization potential. Therefore, current study may have profound application in sustainable agriculture.</p>1 aPuppala, Kumar, Raja1 aNaik, Tejali1 aShaik, Alfina1 aDastager, Syed1 aKumar, Ravi1 aKhire, Jayant1 aDharne, Mahesh  uhttps://library.ncl.res.in/content/evaluation-candida-tropicalis-ncim-3321-extracellular-phytase-having-plant-growth-promoting