02705nas a2200241   4500008004100000022001400041245008400055210006900139260000900208300000800217490000700225520190800232653001502140653001802155653001302173653002902186653003502215653003002250100002502280700002602305700002002331856011202351       2021                        eng d  a1471-222900aPINIR: a comprehensive information resource for Pin-II type protease inhibitors0 aPINIR a comprehensive information resource for PinII type protea  cJUN   a2670 v213 aBackground: Serine protease inhibitors belonging to the Potato type-II Inhibitor family Protease Inhibitors (Pin-II type PIs) are essential plant defense molecules. They are characterized by multiple inhibitory repeat domains, conserved disulfide bond pattern, and a tripeptide reactive center loop. These features of Pin-II type PIs make them potential molecules for protein engineering and designing inhibitors for agricultural and therapeutic applications. However, the diversity in these PIs remains unexplored due to the lack of annotated protein sequences and their functional attributes in the available databases. Results: We have developed a database, PINIR (Pin-II type PIs Information Resource), by systematic collection and manual annotation of 415 Pin-II type PI protein sequences. For each PI, the number and position for signature sequences are specified: 695 domains, 75 linkers, 63 reactive center loops, and 10 disulfide bond patterns are identified and mapped. Database analysis revealed novel subcategories of PIs, species-correlated occurrence of inhibitory domains, reactive center loops, and disulfide bond patterns. By analyzing linker regions, we predict that alternative processing at linker regions could generate PI variants in the Solanaceae family. Conclusion: PINIR (https://pinir.ncl.res.in) provides a web interface for browsing and analyzing the protein sequences of Pin-II type PIs. Information about signature sequences, spatio-temporal expression, biochemical properties, gene sequences, and literature references are provided. Analysis of PINIR depicts conserved species-specific features of Pin-II type PI protein sequences. Diversity in the sequence of inhibitory domains and reactive loops directs potential applications to engineer Pin-II type PIs. The PINIR database will serve as a comprehensive information resource for further research into Pin-II type PIs.10aannotation10aData analysis10aDatabase10aKnowledge representation10aPin-II type protease inhibitor10aProtein sequence analysis1 aYadav, Nikhilesh, K.1 aSaikhedkar, Nidhi, S.1 aGiri, Ashok, P.  uhttps://library.ncl.res.in/content/pinir-comprehensive-information-resource-pin-ii-type-protease-inhibitors