01860nas a2200241 4500008004100000022001400041245012500055210006900180260007300249300001200322490000800334520096100342653002601303653002601329653002401355653002601379100001901405700001501424700001801439700001901457700001701476856012501493 2006 eng d a0376-738800aPoly(urethane methacrylate-co-glycidyl methacrylate)-supported-polypropylene biphasic membrane for lipase immobilization0 aPolyurethane methacrylatecoglycidyl methacrylatesupportedpolypro aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcNOV a395-4030 v2853 a

Polypropylene (PP) was hydrophilized by coating followed by UV curing of a blend of 2-hydroxyethyl methacrylate (HEMA) terminated polyurethane prepolymer and glycidyl methacrylate (GMA). This allows formation of a hydrophobic membrane with increased surface hydrophilicity, biocompatibility and stability. Candida rugosa lipase (CRL) was covalently immobilized on this membrane using 5% glutaraldehyde as a crosslinking agent for post immobilization stabilization of enzyme on membrane. The membrane obtained was placed in a batch membrane reactor where a model esterification of oleic acid with octanol was studied. Under optimum conditions, the biocatalytic membrane gave a specific activity of 796.27 units/mg and 90.26% activity yield. Moreover, there was 85. 10% retention of specific activity. The biocatalytic membrane was observed to retain about 84.23% of its synthetic activity after six cycles. (c) 2006 Elsevier B.V. All rights reserved.

10aCandida rugosa lipase10aEnzyme immobilization10amembrane bioreactor10aurethane methacrylate1 aPujari, N., S.1 aVaidya, Bx1 aBagalkote, S.1 aPonrathnam, S.1 aNene, Sanjay uhttps://library.ncl.res.in/content/polyurethane-methacrylate-co-glycidyl-methacrylate-supported-polypropylene-biphasic-002151nas a2200265 4500008004100000022001400041245014400055210006900199260007300268300001200341490000700353520114100360653002401501653002601525653002801551653001701579100002801596700002501624700002301649700002401672700002501696700001901721700001701740856012801757 2007 eng d a1381-514800aImmobilization of mushroom polyphenol oxidase on poly(allyl glycidyl ether-co-ethylene glycol dimethacrylate) macroporous beaded copolymers0 aImmobilization of mushroom polyphenol oxidase on polyallyl glyci aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcOCT a905-9150 v673 a

Functional, macroporous, beaded copolymers containing epoxy groups were synthesized for immobilization of polyphenol oxidase (PPO) from edible mushroom (Agaricus bisporus). The effect of incorporation of two different sets of monomers such as glycidyl methacrylate (GMA) and allyl glycidyl ether (AGE) and the effect of cross-linking agent ethylene glycol dimethacrylate (EGDM) with varying cross-link densities on binding and expression of mushroom PPO activity were studied. The effect of porogen viz. cyclohexanol and hexanol on PPO immobilization was studied. AGE copolymers with hexanol as a porogen were found to give higher binding and expression of PPO activity than GE polymers. Crosslinking of amino groups of enzyme with 5% glutaraldehyde for 6 h gave a stable binding of PPO on AGE-75(Hex) polymer with storage half-life of approximately 25 days. Under optimum conditions, AGE-75(Hex) polymer gave 70.3% of activity yield while percent retention of PPO activity was found to be 83.5%. Immobilized PPO showed a broader pH, higher temperature and excellent storage stability. (c) 2007 Elsevier Ltd. All rights reserved.

10across-linking agent10aEnzyme immobilization10aEpoxy-activated support10amushroom PPO1 aVaidya, Bhalchandra, K.1 aKarale, Abhijeet, J.1 aSuthar, Hitesh, K.1 aIngavle, Ganesh, C.1 aPathak, Tara, Sankar1 aPonrathnam, S.1 aNene, Sanjay uhttps://library.ncl.res.in/content/immobilization-mushroom-polyphenol-oxidase-polyallyl-glycidyl-ether-co-ethylene-glycol-001941nas a2200241 4500008004100000022001400041245015600055210006900211260007300280300001200353490000700365520100800372653002601380653003301406653002201439653001801461653001701479653001001496100002401506700002901530700001701559856012301576 2008 eng d a1369-703X00aIntegrated process for extraction and purification of alcohol dehydrogenase from Baker's yeast involving precipitation and aqueous two phase extraction0 aIntegrated process for extraction and purification of alcohol de aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAR a414-4200 v383 a

The potential of aqueous two phase extraction for the purification of alcohol dehydrogenase (ADH) from Baker's yeast (Saccharomyces cerevisiae) is demonstrated. An integrated process involving precipitation followed by aqueous two phase extraction (ATPE) is used for effective recovery of the enzyme. This is carried out in two schemes. In the first scheme, ammonium sulphate precipitation is carried out followed by ATPE of the precipitate after suspending it in buffer. While the second scheme involves polyethylene glycol precipitation followed by ATPE of the supernatant. The effect of various process parameters such as molecular weight of polymer, tie line length, phase volume ratio and neutral salt is investigated for enhancing the activity recovery of ADH. First scheme resulted in about 85% enzyme activity recovery of ADH with purification factor of 4.2 while the second scheme about 90% enzyme activity recovery with 6.6-fold purification. (C) 2007 Elsevier B.V. All rights reserved.

10aAlcohol dehydrogenase10aaqueous two phase extraction10aenzyme extraction10aPrecipitation10aPurification10aYeast1 aMadhusudhan, M., C.1 aRaghavarao, K., S. M. S.1 aNene, Sanjay uhttps://library.ncl.res.in/content/integrated-process-extraction-and-purification-alcohol-dehydrogenase-bakers-yeast-001436nas a2200253 4500008004100000022001400041245013600055210006900191260006100260300001200321490000700333520051200340653000600852653003200858653003600890653001400926653002300940653001200963100001600975700002500991700001701016700002301033856012601056 2008 eng d a0959-399300aLipases catalyzed enantioselective hydrolysis of (R,S)-methyl 1,4-benzodioxan-2-carboxylate intermediate for (S)-doxazosin mesylate0 aLipases catalyzed enantioselective hydrolysis of RSmethyl 14benz a233 SPRING STREET, NEW YORK, NY 10013 USAbSPRINGERcAPR a577-5790 v243 a

(S)-1,4-benzodioxan-2-carboxylic acid-1 is used as starting compound for the production of the more effective (S) enantiomer of the drug doxazosin mesylate. The catalytic ability of some commercial lipases for preparations of (S) enantiomer of 1 from (+/-) methyl 1,4-benzodioxin-2-carboxylate-2 is reported. Lipases from bacterial sources were more successful in resolving the ester than those from the yeast lipases. About 85% enantiomerically pure ester was achieved by lipase from alcaligenes sp.

10a110a4-benzodioxan-2-carboxylate10a4-benzodioxan-2-carboxylic acid10adoxazosin10aEnantioselectivity10aLipases1 aVarma, Rita1 aKasture, Sangita, M.1 aNene, Sanjay1 aKalkote, Uttam, R. uhttps://library.ncl.res.in/content/lipases-catalyzed-enantioselective-hydrolysis-rs-methyl-14-benzodioxan-2-carboxylate-002398nas a2200289 4500008004100000022001400041245012800055210006900183260005700252300001400309490000800323520138000331653002001711653001601731653001801747653001801765653001101783653001501794100003201809700002501841700002901866700001701895700002801912700002001940700003001960856011801990 2015 eng d a1438-237700aInfluence of cultivar, ripeness, blanching, drying, irradiation, and pectin recovery on alk(en)ylresorcinols in mango peels0 aInfluence of cultivar ripeness blanching drying irradiation and  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcJUN a1235-12450 v2403 a

The influence of different technological treatments on alk(en)ylresorcinol (AR) contents and pattern in mango peels was evaluated using peels from six different mango cultivars. AR concentrations were shown to highly depend on the cultivar. While high AR concentrations were observed in the analyzed monoembryonic cultivars, AR contents in the polyembryonic cultivar Nam Dokmai were significantly lower. Furthermore, for monoembryonic cultivars, AR levels in the peels of ripe fruits were found to be higher than in unripe ones, while AR levels in cv. Nam Dokmai were independent of the ripeness degree. Convective oven drying resulted in a significant reduction in AR contents when compared to lyophilization. Blanching prior to drying diminished AR yields, thus being unsuitable for satisfactory AR recovery. Gamma irradiation did not significantly affect the AR contents. Since depectinization of mango peels significantly concentrated AR amounts in the depectinized pomace, the consecutive extraction of pectin and ARs allows the dual valorization of mango peels. Considering the treatments investigated, the retention of ARs carrying saturated and unsaturated side chains did not differ under acidic conditions, heating, and irradiation, respectively. Consequently, the 1,3-dihydroxybenzene structure was concluded to be the most labile moiety of the AR molecule.

10aAlkylresorcinol10aBy-products10aMonoembryonic10aPolyembryonic10aPomace10aUltrasound1 aGeerkens, Christian, Hubert1 aMiller-Rostek, Petra1 aMatejka, Anna, Elisabeth1 aNene, Sanjay1 aKammerer, Dietmar, Rolf1 aCarle, Reinhold1 aSchweiggert, Ralf, Martin uhttps://library.ncl.res.in/content/influence-cultivar-ripeness-blanching-drying-irradiation-and-pectin-recovery-002420nas a2200241 4500008004100000022001400041245011100055210006900166260005700235300001400292490000700306520155500313653001701868653001101885653002101896653002101917100002101938700002201959700002601981700001702007700002502024856012902049 2015 eng d a1615-759100aProduction of polyunsaturated fatty acids in recombinant lipomyces starkeyi through submerged fermentation0 aProduction of polyunsaturated fatty acids in recombinant lipomyc a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcJUL a1407-14140 v383 a

Long chain polyunsaturated fatty acids (LCPUFA) are known to play an important role in human health and nutrition. Considering the limitation of LCPUFA sources, it is necessary to search new avenues for their production. Oleaginous yeasts are an attractive target for harvesting single cell oil, mainly because of the ease of cultivation with cheaper raw material. Lipomyces starkeyi is one such oleaginous yeast, which can accumulate oil to the extent of 60 % of its biomass and where genetic transformation can be achieved. In our earlier work, Delta 15 desaturase gene (AEP37840) from flax was transformed into L. starkeyi. In the present work, we report optimization of medium for the production of omega-3 enriched oil from this transformed yeast. A basic medium containing 20 g/l glucose as a carbon source and 10 g/l yeast extract as a nitrogen source was used during fermentation. At regular time intervals, glucose was fed to maintain high C:N ratio (65:10) during fermentation. Under the most favorable conditions, dry biomass and total lipid content were 18 and 7.29 g/l, respectively. Prior to genetic transformation, L. starkeyi contained 56.03 mg/l DHA along with 71.4 mg/l EPA and 42.2 mg/l ALA. Genetic engineering of this yeast resulted in a strain that produced 1080 mg/l DHA (17.4 %) along with 74.28 mg/l EPA and 126.72 mg/l ALA possibly through modification of PUFA biosynthetic pathway. To the best of our knowledge, this is a first report of DHA enrichment and opens up avenues for LCPUFA production through L. starkeyi.

10aFermentation10aLCPUFA10aLipid production10aOleaginous yeast1 aSalunke, Devyani1 aManglekar, Rupali1 aGadre, Ramchandra, V.1 aNene, Sanjay1 aHarsulkar, Abhay, M. uhttps://library.ncl.res.in/content/production-polyunsaturated-fatty-acids-recombinant-lipomyces-starkeyi-through-submerged-001574nas a2200181 4500008004100000245007900041210006900120260000800189300001400197490000700211520095600218100002201174700002401196700001701220700002601237700002301263856010601286 2016 eng d00aKinetic studies on an algal biofilm reactor for raw sewage water treatment0 aKinetic studies on an algal biofilm reactor for raw sewage water cSEP a1629-16350 v393 aA packed-bed algae biofilm reactor was developed using porous and nonporous dual packings. The biofilm was cultivated on reticulated polyurethane foam cubes. The nonporous glass Raschig rings were taken as bed support that helps to remove generated gas from the system. The effect of variables such as column length-to-diameter ratio, catalyst cube dimension, and feed flow rate on the treatment of sewage water was studied. The reaction kinetics indicates that the nutrients uptake rate is dependent on both pore and film diffusion. The kinetics of uptake of nutrients follows a pseudo first-order reaction. From the pseudo reaction rate constant, Thiele modulus and effectiveness factor were calculated and a kinetic model equation for fractional nutrient uptake was developed in terms of operating variables. The model can predict the reaction rate with low deviation. The results obtained may be valuable for large-scale treatment of sewage water.1 aGera, Gayatri, S.1 aYewalkar, Swati, N.1 aNene, Sanjay1 aKulkarni, Bhaskar, D.1 aKamble, Sanjay, P. uhttps://library.ncl.res.in/content/kinetic-studies-algal-biofilm-reactor-raw-sewage-water-treatment-001967nas a2200265 4500008004100000022001400041245008900055210006900144260000800213300001200221490000700233520111500240653001901355653003101374653002501405653001601430653002101446100002901467700001801496700001701514700001901531700002201550700001901572856011001591 2017 eng d a0046-899100aExploiting phosphate-starved cells of scenedesmus SP for the treatment of raw sewage0 aExploiting phosphatestarved cells of scenedesmus SP for the trea cJUN a241-2490 v573 a

Phosphate depletion is one of the favorable ways to enhance the sewage water treatment with the algae, however, detailed information is essential with respect to internal phosphate concentration and physiology of the algae. The growth rate of the phosphate-starved Scenedesmus cells was reduced drastically after 48 h. Indicating cells entered in the stationary phase of the growth cycle. Fourier Transform Infrared analysis of phosphate-starved Scenedesmus cells showed the reduction in internal phosphate concentration and an increase in carbohydrate/phosphate and carbohydrate/lipid ratio. The phosphate-starved Scenedesmus cells, with an initial cell density of, 1 x 10(6) cells mL(-1) shows 87% phosphate and 100 % nitrogen removal in 24 h. The normal Scenedesmus cells need approximately 48 h to trim down the nutrients from wastewater up to this extent. Other microalgae, Ankistrodesmus, growth pattern was not affected due to phosphate starvation. The cells of Ankistrodesmus was able to reduce 71% phosphate and 73% nitrogen within 24 h, with an initial cell density of, 1 x 10(6) cells mL(-1).

10aAnkistrodesmus10aFourier Transform Infrared10aPhosphate starvation10aScenedesmus10aSewage treatment1 aYewalkar-Kulkarni, Swati1 aGera, Gayatri1 aNene, Sanjay1 aPandare, Kiran1 aKulkarni, Bhaskar1 aKamble, Sanjay uhttps://library.ncl.res.in/content/exploiting-phosphate-starved-cells-scenedesmus-sp-treatment-raw-sewage07987nas a2200157 4500008004100000245011600041210006900157260000800226300001100234490000700245520739300252100001907645700001907664700001707683856012907700 2022 eng d00aComparison of hydrodynamic characteristics of Tulsion and Siran immobilised beads in a fluidised bed bioreactor0 aComparison of hydrodynamic characteristics of Tulsion and Siran  cAUG a1024280 v433 a

The fluidised bed reactors are successfully utilised in various bioprocesses to produce high value products using immobilised cells and enzymes. The present work aims at evaluating hydrodynamic characteristics of a three phase fluidised bed bioreactor using Tulsion® A-1X MP, Tulsion® ADS-400 and Tulsion® ADS-800, polystyrene and polyacrylic based three types of free and immobilised cells solid carriers viz. and comparing their performance with Siran® SIKUG41 glass beads. The hydrodynamic parameters such as particle density (cells and beads), drag coefficient, minimum and maximum fluidisation velocity, particle settling velocity were estimated and compared in water and biological media (E2 and MSM) having varying rheological properties. The relationships between drag coefficient (CD)–Reynolds numbers (NRe), superficial liquid velocity (Uf)–void fraction(ε), expansion index(n)–NRe were obtained.

1 aKashid, Shweta1 aJoshi, Kalpana1 aNene, Sanjay uhttps://library.ncl.res.in/content/comparison-hydrodynamic-characteristics-tulsion-and-siran-immobilised-beads-fluidised-bed