02082nas a2200217   4500008004100000245013100041210006900172260000800241300002500249490000600274520129300280100001701573700001901590700002001609700001801629700002101647700002301668700001801691700002601709856012901735       2018                        eng d00aSilk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration0 aSilk fibroin microparticle scaffolds with superior compression m  cMAY  aArticle Number: 72350 v83 aSilk fibroin (SF), a natural polymer produced by Bombyx mori silkworms, has been extensively explored to prepare porous scaffolds for tissue engineering applications. Here, we demonstrate, a scaffold made of SF, which exhibits compression modulus comparable to natural cancellous bone while retaining the appropriate porosities and interconnected pore architecture. The scaffolds also exhibit high resistance to in-vitro proteolytic degradation due to the dominant beta sheet conformation of the SF protein. Additionally, the scaffolds are prepared using a simple method of microparticle aggregation. We also demonstrate, for the first time, a method to prepare SF micro-particles using a Hexafluoroisopropanol-Methanol solvent-coagulant combination. SF microparticles obtained using this method are monodisperse, spherical, non-porous and extremely crystalline. These micro-particles have been further aggregated together to form a 3D scaffold. The aggregation is achieved by random packing of these microparticles and fusing them together using a dilute SF solution. Preliminary in-vitro cell culture and in-vivo implantation studies demonstrate that the scaffolds are biocompatible and they exhibit the appropriate early markers, making them promising candidates for bone regeneration.1 aNisal, Anuya1 aSayyad, Raeesa1 aDhavale, Prachi1 aKhude, Bhakti1 aDeshpande, Rucha1 aMapare, Vidhyashri1 aShukla, Swati1 aVenugopalan, Premnath  uhttps://library.ncl.res.in/content/silk-fibroin-micro-particle-scaffolds-superior-compression-modulus-and-slow-bioresorption02493nas a2200289   4500008004100000245009100041210006900132260000800201300001100209520157300220653003001793653002201823653002101845653002101866653001001887653001901897653001601916653001301932653001701945100002101962700001801983700001902001700002202020700001702042700002602059856011802085       2021                        eng d00aSilk fibroin and ceramic scaffolds: comparative in vitro studies for bone regeneration0 aSilk fibroin and ceramic scaffolds comparative in vitro studies   cAPR  ae102213 a<p>Synthetic bone void fillers based on calcium ceramics are used to fill cavities in the bone and promote bone regeneration. More recently, silk fibroin (SF), a protein polymer obtained from Bombyx mori silkworm, has emerged as a promising material in bone void filling. In this work, we have compared the safety and efficacy of two types of silk fibroin-based bone void fillers with currently used and commercially available ceramic bone void fillers (based on calcium sulphate, beta tricalcium phosphate, and beta tricalcium phosphate with hydroxyapatite). Further, we have also evaluated these two types of SF scaffolds, which have strikingly different structural attributes. The biocompatibility of these scaffolds was comparable as assessed by cytotoxicity assay, cellular adhesion assay, and immunogenic assay. Ability of the scaffolds to support differentiation of human mesenchymal stem cells (hMSCs) into an osteoblastic lineage was also evaluated in an in vitro differentiation experiment using reverse transcriptase polymerase chain reaction analysis. These results revealed that cells cultured on SF scaffolds exhibit higher expression of early to late markers such as Runx2, BMPs, collagen, osterix, osteopontin, and osteocalcin as compared with ceramic-based scaffolds. This observation was further validated by studying the expression of alkaline phosphatase and calcium deposition. We also show that scaffolds made from same material of SF, but characterized by very different pore architectures, have diverse outcome in stem cell differentiation.</p>10abeta tricalcium phosphate10abone regeneration10abone void filler10acalcium sulphate10ahMSCs10ahydroxyapatite10aosteoblasts10ascaffold10aSilk fibroin1 aDeshpande, Rucha1 aShukla, Swati1 aSayyad, Raeesa1 aSalunke, Shalmali1 aNisal, Anuya1 aVenugopalan, Premnath  uhttps://library.ncl.res.in/content/silk-fibroin-and-ceramic-scaffolds-comparative-vitro-studies-bone-regeneration03055nas a2200289   4500008004100000022001400041245009800055210006900153260000800222300001400230490000600244520215300250653002102403653002202424653002102446653001402467653001002481653001202491653001702503100002102520700001802541700001502559700002302574700001702597700002602614856012502640       2022                        eng d  a2373-987800aSilk fibroin microparticle scaffold for use in bone void filling: safety and efficacy studies0 aSilk fibroin microparticle scaffold for use in bone void filling  cMAR  a1226-12380 v83 a<p>
	Silk fibroin (SF) is a natural biocompatible protein polymer extracted from cocoons of silkworm Bombyx mori. SF can be processed into a variety of different forms and shapes that can be used as scaffolds to support bone regeneration. Threedimensional (3D) SF scaffolds have shown promise in bone-void -filling applications. In in vitro studies, it has been demonstrated that a microparticle-based SF (M-RSF) scaffold promotes the differentiation of stem cells into an osteoblastic lineage. The expression of differentiation markers was also significantly higher for M-RSF scaffolds as compared to other SF scaffolds and commercial ceramic scaffolds. In this work, we have evaluated the in vitro and in vivo biocompatibility of M-RSF scaffolds as per the ISO 10993 guidelines in a Good Laboratory Practice (GLP)-certified facility. The cytotoxicity, immunogenicity, genotoxicity, systemic toxicity, and implantation studies confirmed that the M-RSF scaffold is biocompatible. Further, the performance of the MRSF scaffold to support bone formation was evaluated in in vivo bone implantation studies in a rabbit model. Calcium sulfate (CaSO4) scaffolds were chosen as reference material for this study as they are one of the preferred materials for bone-void -filling applications. M-RSF scaffold implantation sites showed a higher number of osteoblast and osteoclast cells as compared to CaSO4 implantation sites indicating active bone remodeling. The number density of osteocytes was double for M-RSF scaffold implantation sites, and these M-RSF scaffold implantation sites were characterized by enhanced collagen deposition, pointing toward a finer quality of the new bone formed. Moreover, the M-RSF scaffold implantation sites had a negligible incidence of secondary fractures as compared to the CaSO4 implantation sites (similar to 50% sites with secondary fracture), implying a reduction in postsurgical complications. Thus, the study demonstrates that the M-RSF scaffold is nontoxic for bone-void -filling applications and facilitates superior healing of fracture defects as compared to commercial calcium-based bone void fillers.</p>
10aBiocompatibility10abiological safety10abone void filler10aISO 1099310aM-RSF10aSerioss10aSilk fibroin1 aDeshpande, Rucha1 aShukla, Swati1 aKale, Amod1 aDeshmukh, Narendra1 aNisal, Anuya1 aVenugopalan, Premnath  uhttps://library.ncl.res.in/content/silk-fibroin-microparticle-scaffold-use-bone-void-filling-safety-and-efficacy-studies