02082nas a2200217   4500008004100000245013100041210006900172260000800241300002500249490000600274520129300280100001701573700001901590700002001609700001801629700002101647700002301668700001801691700002601709856012901735       2018                        eng d00aSilk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration0 aSilk fibroin microparticle scaffolds with superior compression m  cMAY  aArticle Number: 72350 v83 aSilk fibroin (SF), a natural polymer produced by Bombyx mori silkworms, has been extensively explored to prepare porous scaffolds for tissue engineering applications. Here, we demonstrate, a scaffold made of SF, which exhibits compression modulus comparable to natural cancellous bone while retaining the appropriate porosities and interconnected pore architecture. The scaffolds also exhibit high resistance to in-vitro proteolytic degradation due to the dominant beta sheet conformation of the SF protein. Additionally, the scaffolds are prepared using a simple method of microparticle aggregation. We also demonstrate, for the first time, a method to prepare SF micro-particles using a Hexafluoroisopropanol-Methanol solvent-coagulant combination. SF microparticles obtained using this method are monodisperse, spherical, non-porous and extremely crystalline. These micro-particles have been further aggregated together to form a 3D scaffold. The aggregation is achieved by random packing of these microparticles and fusing them together using a dilute SF solution. Preliminary in-vitro cell culture and in-vivo implantation studies demonstrate that the scaffolds are biocompatible and they exhibit the appropriate early markers, making them promising candidates for bone regeneration.1 aNisal, Anuya1 aSayyad, Raeesa1 aDhavale, Prachi1 aKhude, Bhakti1 aDeshpande, Rucha1 aMapare, Vidhyashri1 aShukla, Swati1 aVenugopalan, Premnath  uhttps://library.ncl.res.in/content/silk-fibroin-micro-particle-scaffolds-superior-compression-modulus-and-slow-bioresorption02493nas a2200289   4500008004100000245009100041210006900132260000800201300001100209520157300220653003001793653002201823653002101845653002101866653001001887653001901897653001601916653001301932653001701945100002101962700001801983700001902001700002202020700001702042700002602059856011802085       2021                        eng d00aSilk fibroin and ceramic scaffolds: comparative in vitro studies for bone regeneration0 aSilk fibroin and ceramic scaffolds comparative in vitro studies   cAPR  ae102213 a<p>Synthetic bone void fillers based on calcium ceramics are used to fill cavities in the bone and promote bone regeneration. More recently, silk fibroin (SF), a protein polymer obtained from Bombyx mori silkworm, has emerged as a promising material in bone void filling. In this work, we have compared the safety and efficacy of two types of silk fibroin-based bone void fillers with currently used and commercially available ceramic bone void fillers (based on calcium sulphate, beta tricalcium phosphate, and beta tricalcium phosphate with hydroxyapatite). Further, we have also evaluated these two types of SF scaffolds, which have strikingly different structural attributes. The biocompatibility of these scaffolds was comparable as assessed by cytotoxicity assay, cellular adhesion assay, and immunogenic assay. Ability of the scaffolds to support differentiation of human mesenchymal stem cells (hMSCs) into an osteoblastic lineage was also evaluated in an in vitro differentiation experiment using reverse transcriptase polymerase chain reaction analysis. These results revealed that cells cultured on SF scaffolds exhibit higher expression of early to late markers such as Runx2, BMPs, collagen, osterix, osteopontin, and osteocalcin as compared with ceramic-based scaffolds. This observation was further validated by studying the expression of alkaline phosphatase and calcium deposition. We also show that scaffolds made from same material of SF, but characterized by very different pore architectures, have diverse outcome in stem cell differentiation.</p>10abeta tricalcium phosphate10abone regeneration10abone void filler10acalcium sulphate10ahMSCs10ahydroxyapatite10aosteoblasts10ascaffold10aSilk fibroin1 aDeshpande, Rucha1 aShukla, Swati1 aSayyad, Raeesa1 aSalunke, Shalmali1 aNisal, Anuya1 aVenugopalan, Premnath  uhttps://library.ncl.res.in/content/silk-fibroin-and-ceramic-scaffolds-comparative-vitro-studies-bone-regeneration02917nas a2200313   4500008004100000022001400041245010400055210006900159260000800228300001100236490000800247520193700255653002102192653002602213653001402239653001202253653001102265653001702276653002402293100002102317700001802338700001502356700001902371700002002390700002302410700001702433700002602450856012702476       2025                        eng d  a0141-813000aEngineered silk matrix as a substitute for acellular dermal matrix in breast reconstruction surgery0 aEngineered silk matrix as a substitute for acellular dermal matr  cJUN  a1440170 v3123 a<p>
	Implant-based breast reconstruction is a common standard of care for breast cancer patients following mastectomy. To support implant weight and placement, surgeons utilize autologous tissues, acellular dermal matrices (ADMs), or synthetic meshes. While ADMs provide structural support, they present significant risks, including infection transmission and seroma. Conversely, synthetic meshes exhibit poor cellular adhesion, leading to inadequate tissue integration. To address these challenges, there is a need for a matrix that enhances tissue integration while minimizing infection risks and other complications. Silk fibroin (SF), a natural biopolymer, possesses excellent biocompatibility and mechanical properties. This study introduces a novel engineered silk matrix (ESM) as an advanced solution for soft tissue regeneration, specifically in breast reconstruction surgery. The present study establishes the safety of ESM and evaluates its potential a tissue regeneration matrix through extensive in-vitro and in-vivo analyses. In-vitro assays demonstrated superior cellular adhesion, proliferation of human mammary fibroblast cells (HMFCs), collagen deposition and angiogenesis in ESM compared to collagen matrices and ADMs. Safety assessments, conducted in accordance with ISO 10993 guidelines, confirmed noncytotoxic nature of ESM. Furthermore, subcutaneous implantation revealed no systemic toxicity or adverse tissue reactions. In-vivo studies utilizing a Yorkshire pig model of simulated breast reconstruction surgery, demonstrated superior performance of ESM over collagen matrices in tissue regeneration. The findings showed enhanced fibroblast density, increased collagen deposition, and improved vascularization. These results suggest that ESM is a safer and more effective alternative to ADMs in breast reconstruction, with the potential to revolutionize post-mastectomy care for breast cancer patients.</p>
10aBiocompatibility10aBreast reconstruction10aISO 1099310aPolymer10aSafety10aSilk protein10atissue regeneration1 aDeshpande, Rucha1 aShukla, Swati1 aKale, Amod1 aSayyad, Raeesa1 aKewale, Bhawana1 aDeshmukh, Narendra1 aNisal, Anuya1 aVenugopalan, Premnath  uhttps://library.ncl.res.in/content/engineered-silk-matrix-substitute-acellular-dermal-matrix-breast-reconstruction-surgery02126nas a2200265   4500008004100000022001400041245009700055210006900152260000800221300001100229490000700240520127600247653002101523653001501544653001401559653001701573653001801590100002101608700002401629700001901653700001801672700001701690700002601707856012701733       2025                        eng d  a1616-518700aEngineered silk-dressing for acceleratedwound healing: biocompatibility and efficacy studies0 aEngineered silkdressing for acceleratedwound healing biocompatib  cNOV  ae003230 v253 a<p>
	Advanced wound care dressings are essential for improving clinical outcomes. The present study investigates the wound management potential of a unique dressing fabricated from silk proteins. The dressing was characterized for its physical and structural properties, including surface texture, porosity, fluid absorption capacity, and moisture vapor transmission rate. These parameters have been found to be critical for optimal wound healing. In vivo full thickness wound healing studies in a rat model validated the efficacy of the Silk-dressing compared to conventional cotton gauze and commercial polyurethane foam dressings. Histopathological analysis confirmed improved re-epithelialization, collagen deposition, angiogenesis, and formation of secondary follicles. Key advantages of Silk-dressing included non-adherence, absorption of exudate, maintenance of optimal moisture at wound site and acceleratedwound closure. Biocompatibility studies were also conducted in accordance with ISO 10993 guidelines, demonstrating no cytotoxicity, irritation, sensitization, or pyrogenicity. These findings highlight the potential of this uniquely designed Silk-dressing as a superior alternative for wound management, with a potential to improve clinical outcomes.</p>
10aBiocompatibility10abiopolymer10aISO 1099310aSilk protein10awound Healing1 aDeshpande, Rucha1 aPillai, Lakshmi, R.1 aSayyad, Raeesa1 aShukla, Swati1 aNisal, Anuya1 aVenugopalan, Premnath  uhttps://library.ncl.res.in/content/engineered-silk-dressing-acceleratedwound-healing-biocompatibility-and-efficacy-studies