01886nas a2200193   4500008004100000022001400041245009500055210006900150260000800219300001200227490000700239520124600246100001401492700001801506700001401524700001601538700001401554856012401568       2018                        eng d  a2040-336400aEnhanced and synergistic downregulation of oncogenic miRNAs by self-assembled branched DNA0 aEnhanced and synergistic downregulation of oncogenic miRNAs by s  cJAN  a195-2020 v103 a<p>miRNAs, a group of small non-coding RNA molecules, regulate the expression of many genes involved in various cellular processes. Acute evidence suggests that one miRNA can regulate many genes as its targets, while one gene can be targeted by many miRNAs that co-operatively regulate the gene. Thus, targeting a single miRNA is not sufficient enough to rescue the disease phenotype but it is also essential to target multiple miRNAs simultaneously. This inspired us to design a novel DNA nanostructure that can concurrently downregulate multiple oncomiRNAs. Here we designed a programmable antimiR branched DNA (antimiR-bDNA) nanostructure having antimiRNAs for selective binding to oncomiRNAs miRNA-27a, 96 and 182 which collectively downregulate FOXO1a expression. The antimiR-bDNAs show enhanced stability compared to naked antimiRNAs in serum and are able to knockdown these miRNAs with up to similar to 50% greater repression as compared to antimiRNAs. This synergistic miRNA repression leads to the restoration of FOXO1a protein levels which in turn inhibit G1-S traversion in cancer cells. To the best of our knowledge, this is the first study harnessing the ability of bDNA structures to silence multiple miRNAs simultaneously.</p>1 aNahar, S.1 aNayak, A., K.1 aGhosh, A.1 aSubudhi, U.1 aMaiti, S.  uhttps://library.ncl.res.in/content/enhanced-and-synergistic-downregulation-oncogenic-mirnas-self-assembled-branched-dna02060nas a2200253   4500008004100000022001400041245016900055210006900224260000800293300001400301490000700315520121400322653001601536653001201552653001201564653001701576653001001593100001401603700001401617700001401631700001301645700001701658856013101675       2022                        eng d  a0266-825400aScreening of osmotic stress-tolerant bacteria for plant growth promotion in wheat (Triticum aestivum L.) and brinjal (Solanum melongena L.) under drought conditions0 aScreening of osmotic stresstolerant bacteria for plant growth pr  cNOV  a1286-12920 v753 a<p>
	Drought stress adversely affects plant growth and productivity. Therefore, the application of plant growth-promoting bacteria is a viable option for combating drought resistance in crops. In this study, 144 bacteria were isolated from the Kutch desert soil in Gujarat. Based on osmotic stress tolerance and PGP properties, two strains, Bacillus tequilensis (KS5B) and Pseudomonas stutzeri (KS5C) were tested for their effect on wheat (Triticum aestivum L.) and brinjal (Solanum melongena L.) under drought stress conditions. Inoculation with osmotic stress-tolerant bacteria showed 15 center dot 15-29 center dot 27% enhancement in root length of wheat and 15 center dot 27-32 center dot 59% in brinjal plants. Similarly, the enhancement of shoot length ranged from 14 center dot 72 to 37 center dot 70% for wheat and 59 center dot 39-95 center dot 94% for brinjal plants. Furthermore, the inoculated plants showed significant improvement in chlorophyll content and antioxidant properties such as proline, peroxidase and polyphenol oxidase activity compared to the control. Therefore, the bacterial strains identified in this study can be used to mitigate drought stress and enhance plant biomass.</p>
10aAntioxidant10abrinjal10adrought10aplant growth10aWheat1 aPatel, P.1 aPatil, T.1 aMaiti, S.1 aPaul, D.1 aAmaresan, N.  uhttps://library.ncl.res.in/content/screening-osmotic-stress-tolerant-bacteria-plant-growth-promotion-wheat-triticum-aestivum-l