01870nas a2200181   4500008004100000245010100041210006900142260000800211300001400219490000700233520122200240100002201462700001901484700002001503700002001523700001701543856012801560       2017                        eng d00aIdentification and functionality prediction of pathogenesis-related protein 1 from legume family0 aIdentification and functionality prediction of pathogenesisrelat  cNOV  a2066-20800 v853 aThe production and accumulation of pathogenesis-related (PR) proteins in plants is one of the important responses to biotic and abiotic stress. Large number of identified PR proteins has been categorized into 17 functional families based on their structure, phylogenetics, and biological activities. However, they are not widely studied in legume crops. Using 29 PR1 proteins from Arabidopsis thaliana, as query, here we have predicted 92 candidate PR1 proteins through the PSI-BLAST and HMMER programs. These candidate proteins were comprehensively analyzed with, multiple sequence alignment, domain architecture studies, signal peptide, and motif extraction followed by phylogenetic analysis. Further, response of two candidate PR1 proteins from chickpea against Fusarium oxysporum f.sp.ciceri attack was validated using qRT-PCR followed by their 3D structure prediction. To decipher mode of action for PR1s, docking of pathogen extracellular matrix components along with fungal elicitors was performed with two chickpea PR1 proteins. Based on these findings, we propose carbohydrate to be the unique pathogen-recognition feature for PR1 proteins and beta-glucanase activity via beta-glucan binding or modification.1 aTellis, Meenakshi1 aMathur, Monika1 aGurjar, Gayatri1 aKadoo, Narendra1 aGupta, Vidya  uhttps://library.ncl.res.in/content/identification-and-functionality-prediction-pathogenesis-related-protein-1-legume-family02571nas a2200241   4500008004100000022001400041245009300055210006900148260000800217300000700225490000700232520178400239653001902023653001802042653001602060653001702076653002102093100002102114700002202135700002502157700002202182856012502204       2021                        eng d  a2190-572X00aGlucosinolate induces transcriptomic and metabolic reprogramming in Helicoverpa armigera0 aGlucosinolate induces transcriptomic and metabolic reprogramming  cJAN  a260 v113 a<p>Glucosinolates protect plants from herbivory. Lepidopteran insects have developed resistance to glucosinolates which is well studied. However, the molecular effects of glucosinolate intake on insects are unexplored. To elucidate this, we performed transcriptomics and metabolomics of sinigrin-fed Helicoverpa armigera. Transcriptomics exhibits significant dysregulation of 2375 transcripts, of which 1575 are upregulated and 800 downregulated. Gene Ontology analysis of differentially expressed genes reveals that key hydrolases, oxidoreductases, and transferases are majorly affected. The negative impact of sinigrin is significant and localized in the endomembrane system and mitochondria. It also disturbs various biological processes such as regulation of protein metabolism and cytoskeletal organization. Furthermore, H. armigera putative myrosinase-like enzymes may catalyze the breakdown of sinigrin to allyl isothiocyanate (AITC). AITC targets the electron transport chain causing oxidative stress. KEGG pathway enrichment shows significant upregulation of oxidative phosphorylation, glutathione metabolism and amino acid metabolism. Activation of these pathways induces glutathione synthesis for sinigrin detoxification. Differential gene expression indicates upregulation of glutathione S-transferase and succinate dehydrogenase suggesting mitochondrial impact. Transcriptomics data correlated with metabolomics show changes in serine, methionine, ornithine, and other metabolite levels. It corroborates well with the transcript alterations supporting the increased glutathione production. Thus, our data suggest that sinigrin generates oxidative stress in H. armigera and insects alter their metabolic wiring to overcome sinigrin-mediated deleterious effects.</p>
10aDetoxification10aGlucosinolate10aGlutathione10amitochondria10aOxidative stress1 aJagdale, Shounak1 aTellis, Meenakshi1 aBarvkar, Vitthal, T.1 aJoshi, Rakesh, S.  uhttps://library.ncl.res.in/content/glucosinolate-induces-transcriptomic-and-metabolic-reprogramming-helicoverpa-armigera02575nas a2200325   4500008004100000245017700041210006900218260000800287300000800295490000700303520150700310653001401817653001301831653001601844653001501860653001501875653001501890100001901905700002401924700002201948700001701970700002301987700001802010700001802028700001602046700002102062700001802083700002202101856012602123       2023                        eng d00aComprehensive molecular and clinical investigation of approved Anti-HCV drugs repurposing against SARS-CoV-2 infection: a glaring gap between benchside and bedside medicine0 aComprehensive molecular and clinical investigation of approved A  cMAR  a5150 v113 a<p>
	The limited availability of effective treatment against SARS-CoV-2 infection is a major challenge in managing COVID-19. This scenario has augmented the need for repurposing anti-virals for COVID-19 mitigation. In this report, the anti-SARS-CoV-2 potential of anti-HCV drugs such as daclatasvir (DCV) or ledipasvir (LDP) in combination with sofosbuvir (SOF) was evaluated. The binding mode and higher affinity of these molecules with RNA-dependent-RNA-polymerase of SARS-CoV-2 were apparent by computational analysis. In vitro anti-SARS-CoV-2 activity depicted that SOF/DCV and SOF/LDP combination has IC50 of 1.8 and 2.0 mu M, respectively, comparable to remdesivir, an approved drug for COVID-19. Furthermore, the clinical trial was conducted in 183 mild COVID-19 patients for 14 days to check the efficacy and safety of SOF/DCV and SOF/LDP compared to standard of care (SOC) in a parallel-group, hybrid, individually randomized, controlled clinical study. The primary outcomes of the study suggested no significant difference in negativity after 3, 7 and 14 days in both treatments. None of the patients displayed any worsening in the disease severity, and no mortality was observed in the study. Although, the post hoc exploratory analysis indicated significant normalization of the pulse rate showed in SOF/DCV and SOF/LDP treatment vs. SOC. The current study highlights the limitations of bench side models in predicting the clinical efficacy of drugs that are planned for repurposing.</p>
10aantiviral10aCOVID-1910adaclatasvir10aledipasvir10aSARS-CoV-210asofosbuvir1 aBansode, Sneha1 aSingh, Pawan, Kumar1 aTellis, Meenakshi1 aChugh, Anita1 aDeshmukh, Narendra1 aGupta, Mahesh1 aVerma, Savita1 aGiri, Ashok1 aKulkarni, Mahesh1 aJoshi, Rakesh1 aChaudhary, Dhruva  uhttps://library.ncl.res.in/content/comprehensive-molecular-and-clinical-investigation-approved-anti-hcv-drugs-repurposing00772nas a2200229   4500008004100000022001400041245010200055210006900157260000900226300000700235490000700242653002200249653001200271653001700283653002000300653001400320653001800334100002200352700002000374700001800394856013000412       2024                        eng d  a0250-599100aTrehalase inhibition in Helicoverpa armigera activates machinery for alternate energy acquisition0 aTrehalase inhibition in Helicoverpa armigera activates machinery  cJUL   a740 v4910aEnergy metabolism10aglucose10aMetabolomics10aTranscriptomics10atrehalase10avalidamycin A1 aTellis, Meenakshi1 aMohite, Sharada1 aJoshi, Rakesh  uhttps://library.ncl.res.in/content/trehalase-inhibition-helicoverpa-armigera-activates-machinery-alternate-energy-acquisition