01412nas a2200205 4500008004100000245008700041210006900128260000800197300001600205490000700221520072100228100001500949700002500964700002700989700002001016700002401036700002001060700001701080856010901097 2017 eng d00aSuper-resolution probe to monitor HNO levels in the endoplasmic reticulum of cells0 aSuperresolution probe to monitor HNO levels in the endoplasmic r cNOV a12087-120930 v893 aSelective detection of nitroxyl (HNO), which has recently been identified as a reactive nitrogen species, is a challenging task. We report a BODIPY-based luminescence ON reagent for detection of HNO in aqueous solution and in live RAW 264.7 cells, based on the soft nucleophilicity of the phosphine oxide functionality toward HNO. The probe shows high selectivity to HNO over other reactive oxygen/nitrogen and sulfur species. Luminescence properties of the BODIPY-based chemodosimetric reagent make it an ideal candidate for use as a reagent for super-resolution structured illumination microscopy. The viability of the reagent for biological in vivo imaging application was also confirmed using Artemia as a model.1 aAli, Firoj1 aSreedharan, Sreejesh1 aAshoka, Anila, Hoskere1 aSaeed, Hiwa, K.1 aSmythe, Carl, G. W.1 aThomas, Jim, A.1 aDas, Amitava uhttps://library.ncl.res.in/content/super-resolution-probe-monitor-hno-levels-endoplasmic-reticulum-cells00959nas a2200193 4500008004100000022001400041245006200055210006200117260000900179300001400188490000700202520035000209100002500559700002700584700001900611700001700630700002700647856009100674 2019 eng d a1359-734500aFluorogenic BODIPY molecular rotor as an apoptosis marker0 aFluorogenic BODIPY molecular rotor as an apoptosis marker cJUN  a6902-69050 v553 a

Based on a BODIPY molecular rotor and a zinc-dipicolylamine receptor, we designed a fluorogenic probe for the detection of apoptosis. Being poorly emissive in solution and with healthy cells, it selectively binds phosphatidylserine of early apoptotic cells and internalizes into late apoptotic cells, lighting up its green fluorescence.

1 aAshokkumar, Pichandi1 aAshoka, Anila, Hoskere1 aCollot, Mayeul1 aDas, Amitava1 aKlymchenko, Andrey, S. uhttps://library.ncl.res.in/content/fluorogenic-bodipy-molecular-rotor-apoptosis-marker