01030nas a2200181 4500008004100000245009000041210006900131260000800200300001400208490000700222520038400229100002300613700001500636700003000651700001900681700002400700856012400724 2017 eng d00aDendrimeric amide- and carbamate-linked lysine-based efficient molecular transporters0 aDendrimeric amide and carbamatelinked lysinebased efficient mole cDEC a9579-95840 v153 a

Amide- and carbamate-linked dendrimeric oligomers are reported as molecular transporters. They effectively complex with pDNA and transport it into cells at an efficiency superior to Lipofectamine, when complexation is carried out by incubation overnight. The carbamate-linked K2C is superior to amide-linked K2A; their pDNA complexes have very low associated cytotoxicity.

1 aYadav, Amit, Kumar1 aDey, Namit1 aChattopadhyay, Sabyasachi1 aGanguli, Munia1 aFernandes, Moneesha uhttps://library.ncl.res.in/content/dendrimeric-amide-and-carbamate-linked-lysine-based-efficient-molecular-transporters02335nas a2200253 4500008004100000022001200041245015200053210006900205260000900274300001000283490000700293520141700300653002501717653001301742653002301755653003401778100002601812700002201838700002401860700002401884700002301908700002001931856013001951 2018 eng d a965174800aTripeptides derived from reactive centre loop of potato type II protease inhibitors preferentially inhibit midgut proteases of Helicoverpa armigera0 aTripeptides derived from reactive centre loop of potato type II  cAPR  a17-250 v953 a

Potato type II protease inhibitors (Pin-II PIs) impede the growth of lepidopteran insects by inhibiting serine protease-like enzymes in the larval gut. The three amino acid reactive centre loop (RCL) of these proteinaceous inhibitors is crucial for protease binding and is conserved across the Pin-II family. However, the molecular mechanism and inhibitory potential of the RCL tripeptides in isolation of the native protein has remained elusive. In this study, six peptides corresponding to the RCLs of the predominant Pin-II PIs were identified, synthesized and evaluated for in vitro and in vivo inhibitory activity against serine proteases of the polyphagous insect, Helicoverpa armigera. RCL peptides with sequences PRN, PRY and TRE were found to be potent inhibitors that adversely affected the growth and development of H. armigera. The binding mechanism and differential affinity of the RCL peptides with serine proteases was delineated by crystal structures of complexes of the RCL peptides with trypsin. Residues P1 and P2 of the inhibitors play a crucial role in the interaction and specificity of these inhibitors. Important features of RCL peptides like higher inhibition of insect proteases, enhanced efficacy at alkaline gut pH, longer retention and high stability in insect gut make them suitable molecules for the development of sustainable pest management strategies for crop protection.

10aHelicoverpa armigera10aProtease10aprotease inhibitor10aReactive centre loop peptides1 aSaikhedkar, Nidhi, S.1 aJoshi, Rakesh, S.1 aBhoite, Ashwini, S.1 aMohandasan, Radhika1 aYadav, Amit, Kumar1 aGiri, Ashok, P. uhttps://library.ncl.res.in/content/tripeptides-derived-reactive-centre-loop-potato-type-ii-protease-inhibitors-preferentially01227nas a2200229 4500008004100000022001400041245013800055210006900193260000800262300001400270490000600284520036100290653002500651653002800676653003300704100002300737700002200760700003100782700003300813700002400846856012700870 2019 eng d a2365-654900ap-Nitrophenylcarbonates: a new class of compounds for chemodosimetric colorimetric fluoride anion sensing detectable by the naked eye0 apNitrophenylcarbonates a new class of compounds for chemodosimet cFEB a1830-18330 v43 a

A new class of compounds containing the p-nitrophenylcarbonate motif is reported, that can selectively sense fluoride anions over other halide anions with a detection limit ranging from 0.29 to 0.48 mu M. The fluoride ion acts as nucleophile, leading to the liberation of p-nitrophenol, that is easily detectable and quantifiable colorimetrically.

10acolorimetric sensing10ap-nitrophenyl carbonate10aselective fluoride detection1 aYadav, Amit, Kumar1 aChilukuri, Harsha1 aKumari, Linthoinganbi, Raj1 aKarthikeyan, Muthukumarasamy1 aFernandes, Moneesha uhttps://library.ncl.res.in/content/p-nitrophenylcarbonates-new-class-compounds-chemodosimetric-colorimetric-fluoride-anion