01252nas a2200157   4500008004100000245011000041210006900151260000800220300000900228520066600237100001400903700001700917700002000934700001700954856012300971       2017                        eng d00aEvaluating a new high-throughput twin-arginine translocase assay in bacteria for therapeutic applications0 aEvaluating a new highthroughput twinarginine translocase assay i  cAUG  a1-153 aThe twin-arginine translocase (Tat) pathway is involved in the transport of folded proteins in bacteria, and has been implicated in virulence and pathogenesis. A simple but efficient assay based on the quantification of the exopolysaccharide colanic acid was developed as a new means to study Tat function. Colanic acid contains a methylpentose (l-fucose) component, and its production is directly linked to the Tat pathway through the transport of enzymes involved in polysaccharide biosynthesis. Monitoring of l-fucose levels can be applied for identification of new Tat substrates and high-throughput screening of Tat inhibitors for therapeutic applications.1 aGhosh, D.1 aChougule, S.1 aAvinash, V., S.1 aRamasamy, S.  uhttps://library.ncl.res.in/content/evaluating-new-high-throughput-twin-arginine-translocase-assay-bacteria-therapeutic