02442nas a2200193   4500008004100000245020200041210006900243260000800312300000900320520163600329653002101965653003501986653003802021100001502059700001402074700001502088700001702103856012802120       2018                        eng d00aGenetic engineering approach using early Vinca alkaloid biosynthesis genes led to increased tryptamine and terpenoid indole alkaloids biosynthesis in differentiating cultures of catharanthus roseus0 aGenetic engineering approach using early Vinca alkaloid biosynth  cAUG  a1-113 a<p>Catharanthus roseus today occupies the central position in ongoing metabolic engineering efforts in medicinal plants. The entire multi-step biogenetic pathway of its very expensive anticancerous alkaloids vinblastine and vincristine is fairly very well dissected at biochemical and gene levels except the pathway steps leading to biosynthesis of monomeric alkaloid catharanthine and tabersonine. In order to enhance the plant-based productivity of these pharma molecules for the drug industry, cell and tissue cultures of C. roseus are being increasingly tested to provide their alternate production platforms. However, a rigid developmental regulation and involvement of different cell, tissues, and organelles in the synthesis of these alkaloids have restricted the utility of these cultures. Therefore, the present study was carried out with pushing the terpenoid indole alkaloid pathway metabolic flux towards dimeric alkaloids vinblastine and vincristine production by over-expressing the two upstream pathway genes tryptophan decarboxylase and strictosidine synthase at two different levels of cellular organization viz. callus and leaf tissues. The transformation experiments were carried out using Agrobacterium tumefaciens LBA1119 strain having tryptophan decarboxylase and strictosidine synthase gene cassette. The callus transformation reported a maximum of 0.027% dry wt vindoline and 0.053% dry wt catharanthine production, whereas, the transiently transformed leaves reported a maximum of 0.30% dry wt vindoline, 0.10% catharanthine, and 0.0027% dry wt vinblastine content. © 2017 Springer-Verlag GmbH Austria</p>10aAgroinfiltration10aStrictosidine Synthase (CrSTR)10aTerpenoid Indole Alkaloids (TIAs)1 aSharma, A.1 aVerma, P.1 aMathur, A.1 aMathur, A.K.  uhttps://library.ncl.res.in/content/genetic-engineering-approach-using-early-vinca-alkaloid-biosynthesis-genes-led-increased01737nas a2200217   4500008004100000022001400041245015300055210006900208260000800277300001200285490000700297520097700304100001801281700001501299700001201314700001301326700001501339700002501354700001401379856012601393       2019                        eng d  a0032-388800aEffect of processing conditions on the rheological properties of blends of ultra high molecular weight polyethylene with high‐density polyethylene0 aEffect of processing conditions on the rheological properties of  cDEC  a821-8290 v593 a<p>Blends of high‐density polyethylene (HDPE) with small amounts of ultra‐high molecular weight polyethylene (UHMWPE) were prepared by melt mixing in a twin‐screw microcompounder. Two types of UHMWPE differing in their states of chain entanglement were used. The blend composition, time of mixing, and rotation speed of the screws were varied. Rheological properties of the blends were studied in oscillatory shear and uniaxial elongational tests. Reduction in phase angle measured in dynamic shear rheology and increase in extensional strain hardening were found to be useful indicators for quantifying the extent of mixing of the two components. Although the disentangled UHMWPE showed reasonable mixing with HDPE during typical residence times of melt compounding operations, the entangled UHMWPE remained essentially undissolved. The extent of mixing increased with mixing time and screw speed. POLYM. ENG. SCI., 2018. © 2018 Society of Plastics Engineers</p>
1 aChaudhuri, K.1 aPoddar, S.1 aPol, H.1 aLele, A.1 aMathur, A.1 aRao, G.S., Srinivasa1 aJasra, R.  uhttps://library.ncl.res.in/content/effect-processing-conditions-rheological-properties-blends-ultra-high-molecular-weight