01030nas a2200157   4500008004100000245008800041210006900129260000800198300001400206490000600220520046600226100002400692700001900716700002100735856011600756       2016                        eng d00aDraft genome sequence of arthrobacter enclensis NCIM 5488T for secondary metabolism0 aDraft genome sequence of arthrobacter enclensis NCIM 5488T for s  cJUN  ae00497-160 v43 aHere, we report the draft genome sequence of Arthrobacter enclensis NCIM 5488T, an actinobacterium isolated from a marine sediment sample from Chorao Island, Goa, India. This draft genome sequence consists of 4,226,231 bp with a G+C content of 67.08%, 3,888 protein-coding genes, 50 tRNAs, and 10 rRNAs. Analysis of the genome using bioinformatics tools such as antiSMASH and NaPDoS showed the presence of many unique natural product biosynthetic gene clusters.1 aNeurgaonkar, P., S.1 aDharne, M., S.1 aDastager, S., G.  uhttps://library.ncl.res.in/content/draft-genome-sequence-arthrobacter-enclensis-ncim-5488t-secondary-metabolism02575nas a2200181   4500008004100000245013100041210006900172260000800241300001100249490000600260520190300266100001802169700002402187700001702211700002102228700001902249856012502268       2016                        eng d00aPeeping into genomic architecture by re-sequencing of Ochrobactrum intermedium M86 strain during laboratory adapted conditions0 aPeeping into genomic architecture by resequencing of Ochrobactru  cJUN  a 72-760 v83 aAdvances in de novo sequencing technologies allow us to track deeper insights into microbial genomes for restructuring events during the course of their evolution inside and outside the host. Bacterial species belonging to Ochrobactrum genus are being reported as emerging, and opportunistic pathogens in this technology driven era probably due to insertion and deletion of genes. The Ochrobactrum intermedium M86 was isolated in 2005 from a case of non-ulcer dyspeptic human stomach followed by its first draft genome sequence in 2009. Here we report re-sequencing of O. intermedium M86 laboratory adapted strain in terms of gain and loss of genes. We also attempted for finer scale genome sequence with 10 times more genome coverage than earlier one followed by comparative evaluation on Ion PGM and Illumina MiSeq. Despite their similarities at genomic level, lab-adapted strain mainly lacked genes encoding for transposase protein, insertion elements family, phage tail-proteins that were not detected in original strain on both chromosomes. Interestingly, a 5 kb indel was detected in chromosome 2 that was absent in original strain mapped with phage integrase gene of Rhizobium spp. and may be acquired and integrated through horizontal gene transfer indicating the gene loss and gene gain phenomenon in this genus. Majority of indel fragments did not match with known genes indicating more bioinformatic dissection of this fragment. Additionally we report genes related to antibiotic resistance, heavy metal tolerance in earlier and re-sequenced strain. Though SNPs detected, there did not span urease and flagellar genes. We also conclude that third generation sequencing technologies might be useful for understanding genomic architecture and re-arrangement of genes in the genome due to their ability of larger coverage that can be used to trace evolutionary aspects in microbial system. 1 aGohil, K., N.1 aNeurgaonkar, P., S.1 aParanjpe, A.1 aDastager, S., G.1 aDharne, M., S.  uhttps://library.ncl.res.in/content/peeping-genomic-architecture-re-sequencing-ochrobactrum-intermedium-m86-strain-during02169nas a2200193   4500008004100000245011600041210006900157260000800226300001400234490000700248520146800255100001901723700002201742700002201764700002201786700002101808700001801829856012801847       2017                        eng d00aMicrovirga indica sp. Nov., an arsenite-oxidizing alphaproteobacterium, isolated from metal industry waste soil0 aMicrovirga indica sp Nov an arseniteoxidizing alphaproteobacteri  cSEP  a3525-35310 v673 aA novel Gram-stain-negative bacterium, strain S-MI1bT, belonging to the genus Microvirga was isolated from a metal industry waste soil sample in Pirangut village, Pune District, Maharashtra, India. Cells were non-spore-forming, small rod-shapes, motile and strictly aerobic with light-pink colonies. The strain grew in 0-7.0 % (w/v) NaCl and at 25-45 °C, with optimal growth at 40 °C. The predominant fatty acids detected were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and C19 : 0 cyclo ω8c. The predominant isoprenoid quinone was Q-10. The G+C content was 67.2 mol% and DNA-DNA relatedness values between strain S-MI1bTand Microvirga subterranea DSM 14364T and Microvirgaaerophila 5420S-12T were 53.9 and 54.8 %, respectively. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain S-MI1bT is a member of the genus Microvirga, with greatest sequence similarities of 97.7 and 97.4 % with M. subterranea DSM 14364T and M.aerophila 5420S-12T, respectively. Phylogenetic analysis showed that strain S-MI1bT forms a clade with the type strain of M. subterranea DSM 14364T, and was readily distinguishable from it due to various phenotypic characteristics. The combination of genotypic and phenotypic data suggests that the isolate represents a novel species of the genus Microvirga, for which the name Microvirga indica sp. nov. is proposed. The type strain is S-MI1bT (=NCIM-5595T=KACC 18792T=BCRC 80972T)1 aTapase, S., R.1 aMawlankar, R., B.1 aSundharam, S., S.1 aKrishnamurthi, S.1 aDastager, S., G.1 aKodam, K., M.  uhttps://library.ncl.res.in/content/microvirga-indica-sp-nov-arsenite-oxidizing-alphaproteobacterium-isolated-metal-industry