02102nas a2200241 4500008004100000022001400041245006200055210006000117260007400177300001200251490000800263520130200271653001601573653002001589653001501609100002601624700002501650700001901675700003001694700002001724700002401744856009201768 2014 eng d a0003-607200aBacillus enclensis sp nov., isolated from sediment sample0 aBacillus enclensis sp nov isolated from sediment sample aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcJAN a199-2060 v1053 a

A novel bacterial strain, designated SGD-1123(T) was isolated from Chorao Island, in Goa Province, India. The strain was found to be able to grow at 15-42 A degrees C, pH 5-12 and 0-12 % (w/v) NaCl. The whole cell hydrolysates were found to contain meso-diaminopimelic acid, galactose and arabinose. The major fatty acids were identified as iso-C-15:0 and anteiso-C-15:0, MK-7 was identified as the predominant menaquinone and the predominant polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The genomic DNA G+C content was determined to be 44.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate within the genus Bacillus and further revealed that strain SGD-1123(T) had highest sequence similarity with Bacillus aquimaris, and forms a separate clade with its closest relatives i.e. B. aquimaris, Bacillus vietnamensis and Bacillus marisflavi, with which it shares 94.5, 94.1 and 94.1 % similarity respectively. The phylogenetic, chemotaxonomic and phenotypic analyses indicated that strain SGD-1123(T) represents a novel species within the genus Bacillus, for which the name Bacillus enclensis is proposed. The type strain is SGD-1123(T) (NCIM 5450(T)=CCTCC AB 2011125(T)).

10aBacillus sp10aMarine sediment10aPolyphasic1 aDastager, Syed, Gulam1 aMawlankar, Rahul, R.1 aTang, Shan-Kun1 aSrinivasan, Krishnamurthi1 aRamana, Venkata1 aShouche, Yogesh, S. uhttps://library.ncl.res.in/content/bacillus-enclensis-sp-nov-isolated-sediment-sample-002348nas a2200253 4500008004100000022001400041245006700055210006500122260007400187300001200261490000800273520149000281653001801771653002201789653002401811100002601835700002501861700003001886700001901916700001801935700002001953700002401973856009701997 2014 eng d a0003-607200aFictibacillus enclensis sp nov., isolated from marine sediment0 aFictibacillus enclensis sp nov isolated from marine sediment aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcMAR a461-4690 v1053 a

A novel Gram-positive strain, designated NIO-1003(T), was isolated from a marine sediment sample collected from the Chorao Island, Goa Provence, India. Strain NIO-1003(T) was found to be strictly aerobic, motile, endospore-forming rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NIO-1003(T) belongs to the genus Fictibacillus and to be most closely related to Fictibacillus rigui KCTC 13278(T), Fictibacillus solisalsi KCTC 13181(T) and Fictibacillus barbaricus DSM 14730(T) with 98.2, 98.0 and 97.2 % similarity and 25, 28, 39 nucleotide differences respectively. Strain NIO-1003(T) was characterized by having cell-wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the predominant menaquinone. The polar lipid profile exhibited the major compounds diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. In addition, minor amounts of an aminophospholipid were detected. The major fatty acids were identified as ai-C-15:0, iso-C-15:0, ai-C-17:0 and C-16:0, supporting the grouping of strain NIO-1003(T) into the family Bacillaceae. The DNA G+C content of strain NIO-1003(T) was determined to be 42.6 mol%. On the basis of phenotypic properties, phylogeny and DNA-DNA hybridisation analysis, strain NIO-1003(T) is considered to represent a novel species of the genus Fictibacillus for which the name Fictibacillus enclensis sp. nov. is proposed. The type strain is NIO-1003(T) (= NCIM 5458(T) = DSM 25142(T)).

10aChorao Island10aFictibacillus sp.10aPolyphasic taxonomy1 aDastager, Syed, Gulam1 aMawlankar, Rahul, R.1 aSrinivasan, Krishnamurthi1 aTang, Shan-Kun1 aLee, Jae-Chan1 aRamana, Venkata1 aShouche, Yogesh, S. uhttps://library.ncl.res.in/content/fictibacillus-enclensis-sp-nov-isolated-marine-sediment-002325nas a2200193 4500008004100000022001400041245006000055210005800115260011800173300001200291490000700303520162400310100002601934700001901960700003001979700001802009700001602027856008802043 2014 eng d a1466-502600aKocuria indica sp nov., isolated from a sediment sample0 aKocuria indica sp nov isolated from a sediment sample aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcMAR a869-8740 v643 a

An actinobacterial strain was isolated from a sediment sample from Chorao Island, in Goa province, India, and subjected to a taxonomic investigation. The isolate, designated NIO-1021(T), was a Gram-stain positive, aerobic, non-motile and coccoid. Strain NIO-1021(T) was identified as a member of the genus Kocuria by a polyphasic approach. Strain NIO-1021(T) could be differentiated from other members of the genus Kocuria on the basis of physiology and 16S rRNA gene sequence analysis. The 16S rRNA gene sequence similarity of strain NIO-1021(T) to the most closely related species, Kocuria marina KCTC 9943(T), was 98.6% with 19 nt differences). Furthermore, DNA-DNA hybridization analysis revealed that the novel strain had lower relatedness with the type strains of other members of the genus Kocuria. The strain formed a monophyletic clade with K. marina with 100% bootstrap values. The major phospholipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified lipids. The predominant menaquinone was MK-7(H-2). The major fatty acids were anteiso-C-15:0, iso-C-(16:0) and anteiso-C-17:0. The DNA G+C content of strain NIO-1021(T) was 60.5 mol%. Chemotaxonomic and phylogenetic properties of the strain were consistent with its classification as representing a member of the genus Kocuria. On the basis of phenotypic, chemotypic and molecular characteristics, strain NIO-1021(T) is considered to represent a novel species of the genus Kocuria, for which the name Kocuria indica sp. nov. is proposed, with strain NIO-1021(T) (=NCIM 5455(T)=DSM 25126(T)=CCTCC AA 209050(T)) as the type strain.

1 aDastager, Syed, Gulam1 aTang, Shan-Kun1 aSrinivasan, Krishnamurthi1 aLee, Jae-Chan1 aLi, Wen-Jun uhttps://library.ncl.res.in/content/kocuria-indica-sp-nov-isolated-sediment-sample-002334nas a2200217 4500008004100000022001400041245006700055210006500122260011800187300001400305490000700319520152900326100002501855700001701880700002401897700002401921700001801945700003001963700002601993856009702019 2015 eng d a1466-502600aMicrobacterium enclense sp nov., isolated from sediment sample0 aMicrobacterium enclense sp nov isolated from sediment sample aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcJUL a2064-20700 v653 a

A novel bacterium (strain NIO-1002(T)) belonging to the genus Microbacterium was isolated from a marine sediment sample in Chorao Island, Goa Province, India. Its morphology, physiology, biochemical features and 16S rRNA gene sequence were characterized. Cells of this strain were Gram-stain-positive, non-motile, non-spore-forming rods that formed yellow-pigmented colonies. It grew in 0-12 % (w/v) NaCl and at 25-37 degrees C, with optimal growth at 30 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NIO-1002(T) is associated with members of the genus Microbacterium, with highest sequence similarity with Microbacterium hominis CIP 105731(T) (98.1%) and Microbacterium testaceum KCTC 9103(T) (98.0 %). Within the phylogenetic tree, this novel strain shared a branching point with M. hominis CIP 105731(T). The DNA G+C content was 66.5 mol% and DNA DNA hybridization relatedness between NI-1002(T), M. hominis CIP 105731(T) and M. testaceum KCTC 9103(T) was 39.0 +/- 2.0 % and 41.0 +/- 2.0 %, respectively. The major fatty acids were ai-C-15:0, i-C-16:0 and al-C-17:0 and the diamino acid in the cell-wall peptidoglycan of NIO-1002(T) was lysine. Data obtained from DNA-DNA hybridization and chemotaxonomic phenotypic analysis support the conclusion that strain NIO-1002(T) represents a novel species within the genus Microbacterium. The name Microbacterium enclense sp. nov. is proposed, with NIO-1002(T) (=NCIM 5454(T)=DSM 25125(T)=CCTCC AB 2011120(T)) as the type strain.

1 aMawlankar, Rahul, R.1 aMual, Poonam1 aSonalkar, Vidya, V.1 aThorat, Meghana, N.1 aVerma, Ashish1 aSrinivasan, Krishnamurthi1 aDastager, Syed, Gulam uhttps://library.ncl.res.in/content/microbacterium-enclense-sp-nov-isolated-sediment-sample-000625nas a2200181 4500008004100000022001400041245008400055210006900139260000800208300000900216490000700225100002300232700001900255700003000274700001800304700001600322856010500338 2017 eng d a1466-502600aKocuria indica sp. nov., isolated from a sediment sample (vol 64, pg 869, 2014)0 aKocuria indica sp nov isolated from a sediment sample vol 64 pg  cMAY a16200 v671 aDastager, Syed, G.1 aTang, Shan-Kun1 aSrinivasan, Krishnamurthi1 aLee, Jae-Chan1 aLi, Wen-Jun uhttps://library.ncl.res.in/content/kocuria-indica-sp-nov-isolated-sediment-sample-vol-64-pg-869-201400722nas a2200205 4500008004100000022001400041245009200055210006900147260000800216300000900224490000700233100002500240700001700265700002400282700002400306700001800330700003000348700002300378856011500401 2019 eng d a1466-502600aMicrobacterium enclense sp. nov., isolated from sediment sample (vol 65, pg 2064, 2015)0 aMicrobacterium enclense sp nov isolated from sediment sample vol cMAY a15280 v691 aMawlankar, Rahul, R.1 aMual, Poonam1 aSonalkar, Vidya, V.1 aThorat, Meghana, N.1 aVerma, Ashish1 aSrinivasan, Krishnamurthi1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/microbacterium-enclense-sp-nov-isolated-sediment-sample-vol-65-pg-2064-2015