01827nas a2200193   4500008004100000245005800041210005500099260000800154300001400162490000700176520126500183100001501448700001601463700001501479700002001494700001501514700001901529856008501548       2017                        eng d00aAllostreptomyces indica sp. nov., isolated from India0 aAllostreptomyces indica sp nov isolated from India  cOCT  a1000-10030 v703 aA novel actinobacterium, designated strain YIM 75704T, was isolated from a limestone quarry located at Gulbarga, Karnataka, India. The novel strain has showed typical morphological and chemotaxonomic characteristics of the family Streptomycetaceae. Comparison of 16S rRNA gene sequences indicated that this strain represents a novel member of the family Streptomycetaceae and exhibited 99.0% 16S rRNA gene sequence similarities with the type species of the recently described novel genus Allostreptomyces, that is, Allostreptomyces psammosilenae, whereas other species of Streptomyces were below 95% sequence similarity. The cell hydrolysates contained the LL-isomer of diaminopimelic acid and the predominant quinones were MK-9 (H 6, H 8 and H 4). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylinositolmannosides and three unknown phospholipids. The DNA G+C content was 75.0 mol%. A polyphasic study of the strain with morphological, phenotypic, phylogenetic and with DNA-DNA hybridization evidence with related members showed that this strain represents novel species of Allostreptomyces for which the name Allostreptomyces indica sp. nov., is proposed. The type strain is YIM 75704 T (= DSM 41985T =CCTCC AA 209051T = NCIM 5485T).1 aSahu, A.K.1 aQuadri, S.R1 aAgasar, D.1 aRuwaili, J., A.1 aJun-Li, W.1 aDastager, S.G.  uhttps://library.ncl.res.in/content/allostreptomyces-indica-sp-nov-isolated-india02393nas a2200145   4500008004100000245012200041210006900163260000800232300001600240490000600256520181900262100001702081700001902098856013002117       2018                        eng d00aHigh yield production of cellulose by a komagataeibacter rhaeticus PG2 strain isolated from pomegranate as a new host0 aHigh yield production of cellulose by a komagataeibacter rhaetic  cAUG  a29797-298050 v83 aGluconacetobacter xylinus is a well-known organism that produces bacterial cellulose (BC). The present study was undertaken to find an alternative bacteria from a collection of 216 bacterial isolates, which were isolated from different rotten fruits and fermented beverages, to find a better producer of bacterial cellulose. We obtained a potent strain, which produced a high yield of BC from a rotten pomegranate sample, and was further identified as Komagataeibacter rhaeticus strain PG2 using 16S rRNA gene sequence analysis. To date, only two strains of Komagataeibacter rhaeticus are known to produce BC, and these were mainly isolated from a fermented beverage, kombucha. For the first time, we have isolated a BC producing Komagataeibacter rhaeticus strain PG2 from a rotten pomegranate sample. The new host environment and the substrate utilization pattern of strain PG2 reveal efficient bacterial cellulose production. Hestrin–Schramm (HS) liquid media containing glycerol as a carbon source resulted in the highest BC production (∼6.9 g L−1). A further increased yield of BC (∼8.7 g L−1) was obtained by using 3% (w/v) glycerol concentration, and this BC yield is the highest reported among any of the known Komagataeibacter rhaeticus strains reported. A detailed physico-chemical characterization of the BC membrane obtained from glycerol (Gly-BC) and glucose (Glc-BC) was performed. Interestingly, Gly-BC is found to be more compact and more crystalline in its nature compared to Glc-BC. The present study reveals the isolation of an efficient BC synthesizing strain using glycerol as a low-cost carbon source, confirming the economic feasibility of BC production. The structural characteristics of the BC membrane produced by glycerol were found to be more suitable for various applications.1 aThorat, M.N.1 aDastager, S.G.  uhttps://library.ncl.res.in/content/high-yield-production-cellulose-komagataeibacter-rhaeticus-pg2-strain-isolated-pomegranate