02384nas a2200217   4500008004100000022001400041245011100055210006900166260009000235300002600325490000600351520153400357100001901891700002101910700002001931700001901951700002301970700001801993700002402011856013102035       2015                        eng d  a2045-232200aIsolation, characterization and antifungal docking studies of wortmannin isolated from penicillium radicum0 aIsolation characterization and antifungal docking studies of wor  aMACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLANDbNATURE PUBLISHING GROUPcJUL  aArticle Number: 119480 v53 a<p>During the search for a potent antifungal drug, a cell-permeable metabolite was isolated from a soil isolate taxonomically identified as Penicillium radicum. The strain was found to be a potent antifungal agent. Production conditions of the active compound were optimized and the active compound was isolated, purified, characterized and identified as a phosphoinositide 3-kinase (PI3K) inhibitor, commonly known as wortmannin (Wtmn). This is very first time we are reporting the production of Wtmn from P. radicum. In addition to its previously discovered anticancer properties, the broad spectrum antifungal property of Wtmn was re-confirmed using various fungal strains. Virtual screening was performed through molecular docking studies against potential antifungal targets, and it was found that Wtmn was predicted to impede the actions of these targets more efficiently than known antifungal compounds such as voriconazole and nikkomycin i.e. 1) mevalonate-5-diphosphate decarboxylase (1FI4), responsible for sterol/isoprenoid biosynthesis; 2) exocyst complex component SEC3 (3A58) where Rho-and phosphoinositide-dependent localization is present and 3) Kre2p/Mnt1p a Golgi alpha1,2-mannosyltransferase (1S4N) involved in the biosynthesis of yeast cell wall glycoproteins). We conclude that Wtmn produced from P. radicum is a promising lead compound which could be potentially used as an efficient antifungal drug in the near future after appropriate structural modifications to reduce toxicity and improve stability.</p>1 aSingh, Vineeta1 aPraveen, Vandana1 aTripathi, Divya1 aHaque, Shafiul1 aSomvanshi, Pallavi1 aKatti, S., B.1 aTripathi, C., K. M.  uhttps://library.ncl.res.in/content/isolation-characterization-and-antifungal-docking-studies-wortmannin-isolated-penicillium-1