02043nas a2200157   4500008004100000245010700041210006900148260003100217300001200248520141900260100002001679700001801699700002101717700002201738856012501760       2017                        eng d00aChapter 5: Experimental and computational approaches to study membranes and lipid-protein interactions0 aChapter 5 Experimental and computational approaches to study mem  bRoyal Society of Chemistry  a137-1603 a<p>Biological membranes are complex two-dimensional, non-covalent assemblies of a diverse variety of lipids and proteins. A hallmark of membrane organization is varying degrees of spatiotemporal heterogeneity spanning a wide range. Membrane proteins are implicated in a wide variety of cellular functions, and comprise ∼30% of the human proteome and ∼50% of the current drug targets. Their interactions with membrane lipids are recognized as crucial elements in their function. In this article, we provide an overview of experimental and theoretical approaches to analyze membrane organization, dynamics, and lipid-protein interactions. In this context, we highlight the wide range of time scales that membrane events span, and approaches that are suitable for a given time scale. We discuss representative fluorescence-based approaches (FRET and FRAP) that help to address questions on lipid-protein and protein-cytoskeleton interactions in membranes. In a complimentary fashion, we discuss computational methods, atomistic and coarse-grain, that are required to address a given membrane problem at an appropriate scale. We believe that the synthesis of knowledge gained from experimental and computational approaches will enable us to probe membrane organization, dynamics, and interactions at increasing spatiotemporal resolution, thereby providing a robust model for the membrane in health and disease.</p>1 aSengupta, Durba1 aKumar, Aditya1 aPrasanna, Xavier1 aChattopadhyay, A.  uhttps://library.ncl.res.in/content/chapter-5-experimental-and-computational-approaches-study-membranes-and-lipid-protein00457nas a2200133   4500008004100000245005100041210005100092300001100143100002000154700001800174700002000192700002800212856008300240       2020                        eng d00aMolecular determinants of GPCR oligomerization0 aMolecular determinants of GPCR oligomerization  a97-1081 aMohole, Madhura1 aKumar, Aditya1 aSengupta, Durba1 aChattopadhyay, Amitabha  uhttps://library.ncl.res.in/content/molecular-determinants-gpcr-oligomerization