01815nas a2200169 4500008004100000245015000041210006900191260000800260300001200268490000700280520116000287100002501447700001601472700001801488700001901506856012001525 2018 eng d00aDephytinizing and probiotic potentials of saccharomyces cerevisiae (NCIM 3662) strain for amelioration of nutritional quality of functional foods0 aDephytinizing and probiotic potentials of saccharomyces cerevisi cMAR a604-6170 v113 aIncrease of undigested complexes of phytic acid in food is gaining serious attention to overcome nutritional challenges due to chelation effects. We investigated soil-borne yeast phytase from Saccharomyces cerevisiae (NCIM 3662) for dephytinization of foods, probiotic properties, and process development. The strain produced 45 IU/DCG by cell-bound phytase in an unoptimized medium was increased fourfold (164 IU/DCG) in 12 h using statistical media optimization. The process was scaled-up up to 10-L fermenter scale with increased phytase productivity of 6.4 IU/DCG/h as compared to the lab scale. The strain displayed probiotic characteristics like tolerance to artificial gastric acid conditions, hydrophobicity, autoaggregation, coaggregation, and bile salt hydrolase (BSH) activity. Further, it could dephytinize (removal of phytic acid; an anti-nutritional factor) functional foods like ragi (finger millet) flour, soya flour, chickpea flour, and poultry animal feed. A combination of cell-bound dephytinizing phytase and nutrition-ameliorating probiotic traits of S. cerevisiae (NCIM 3662) presents profound applications in food technology sector.1 aPuppala, Kumar, Raja1 aKumar, Ravi1 aKhire, Jayant1 aDharne, Mahesh uhttps://library.ncl.res.in/content/dephytinizing-and-probiotic-potentials-saccharomyces-cerevisiae-ncim-3662-strain01129nas a2200229 4500008004100000245012400041210006900165260000800234520028600242100001500528700002500543700001600568700002700584700002200611700001900633700001900652700002300671700001800694700003100712700002400743856013200767 2019 eng d00aDraft genome sequence of freshwater-derived streptomyces sp. strain BPSDS2, isolated from damte stream, northeast India0 aDraft genome sequence of freshwaterderived streptomyces sp strai cOCT3 a

We report the draft genome sequence of Streptomyces sp. strain BPSDS2, isolated from freshwater sediments in Northeast India. The draft genome has a size of 8.27 Mb and 7,559 protein-coding sequences.

1 aZothanpuia1 aPassari, Ajit, Kumar1 aDeka, Vinay1 aRajput, Lakshmi, P. M.1 aPriya, Purbajyoti1 aDharne, Mahesh1 aDastager, Syed1 aMathew, Oommen, K.1 aHashem, Abeer1 aAllah, Elsayed, Fathi Abd_1 aSingh, Bhim, Pratap uhttps://library.ncl.res.in/content/draft-genome-sequence-freshwater-derived-streptomyces-sp-strain-bpsds2-isolated-damte-stream03345nas a2200253 4500008004100000022001400041245013800055210006900193260000800262520238100270653003102651653002102682653003102703653002302734653001902757653003702776100002702813700002302840700002102863700001902884700002802903700003002931856013002961 2024 eng d a1867-130600aDietary supplementation of lactococcus lactis subsp. lactis BIONCL17752 on growth performance, and gut microbiota of broiler chickens0 aDietary supplementation of lactococcus lactis subsp lactis BIONC cJUN3 a

The rapid rise of antimicrobial resistance (AMR) is a global concern, being triggered by the overuse or misuse of antibiotics in poultry farming sector. We evaluated Lactococcus lactis subsp. lactis BIONCL17752 strain, and characterized its probiotic potential to endure hostile gastrointestinal conditions. Genome sequencing analysis revealed probiotics traits, and gene clusters involved in bacteriocins, lactococcin A, and sactipeptides production. The absence of genes for antibiotic resistance, virulence, and biogenic amine production indicates the potential of probiotic strain. The BIONCL17752 strain was explored for antibiotic-free feed supplement for growth promotor in broiler chicken. The feed supplemented with 4 x 109 CFU/kg of probiotic strain, in combination with various concentrations of fructooligosaccharides (FOS) 1.0, 2.5, and 5.0 kg/tonne in starter, grower, and finisher diets, respectively. A significant improvement of body weight 152 to 171 g/bird (p < 0.05), and a low feed conversion ratio (FCR) of 1.62, was achieved without using synthetic antibiotics for growth promotion. The results of biochemical, hematological, and histological examinations showed normal features, indicating that the treatment had no harmful effects on the bird's health. Reduced levels of cholesterol, triglycerides, high-density lipoprotein (HDL), and low-density lipoprotein (LDL) in serum are an indication of the health benefits for the treated birds. Microbial community analysis of fecal samples of poultry birds exhibited a higher abundance of Bacteroidetes, Firmicutes, Proteobacteria, Actinobacteria, and Fusobacteria. Probiotic treatment resulted in reduced Firmicutes and increased Bacteroidetes (F/B ratio) in the broiler's gut which highlights the benefits of probiotic dietary supplements. Importantly, the probiotic-fed group exhibited a high abundance of carbohydrate-active enzymes (CAZyme) such as glycoside hydrolases (GH), glycoside transferases (GT), and carbohydrate-binding module (CBM) hydrolases which are essential for the degradation of complex sugar molecules. The probiotic potential of the BIONCL17752 strain contributes to broilers' health by positively affecting intestinal microbiota, achieving optimal growth, and lowering mortality, demonstrating the economic benefits of probiotic treatment in organic poultry farming.

10aAntibiotic growth promotor10aBroiler chickens10aFunctional genome analysis10aGrowth performance10aGut microbiota10aLactococcus lactis subsp. lactis1 aNavale, Vishwambar, D.1 aYadav, Rakeshkumar1 aKhilari, Ajinkya1 aDharne, Mahesh1 aShanmugam, Dhanasekaran1 aVamkudoth, Koteswara, Rao uhttps://library.ncl.res.in/content/dietary-supplementation-lactococcus-lactis-subsp-lactis-bioncl17752-growth-performance-and02874nas a2200229 4500008004100000245011900041210006900160260000800229490000700237520206500244653001902309653002302328653001502351653002602366653002302392100001902415700001902434700001902453700002202472700001902494856013102513 2025 eng d00aDeciphering the comprehensive microbiome of glacier-fed Ganges and functional aspects: implications for one health0 aDeciphering the comprehensive microbiome of glacierfed Ganges an cAUG0 v133 a

Glacier-fed rivers are significant ecological components of the river catchments, yet their microbial diversity and the associated antimicrobial potential remain underexplored. The Ganges is a glacier-fed river of immense cultural, religious, and ecological significance that supports over 400 million people downstream, providing essential water for agriculture, industry, and daily use. Despite its importance, the microbial community composition and antimicrobial potential, across its relatively pristine origin, remain largely underexplored. One possible explanation for this could be the lower microbial load in the upstream glacier-fed region, which likely results in a reduced DNA yield, insufficient for whole-metagenome sequencing, in contrast to the more biologically diverse and nutrient-rich lower reaches. In this study, we developed an efficient DNA extraction and amplification method using low-input DNA to sequence the microbiome from sediments of the glacier-fed Ganges River in pre-monsoon and post-monsoon over 2 years. Taxonomic and functional diversity of bacterial and viral (phage) communities were analyzed, together with the seasonal variations in their composition. Significant differences in microbial communities were observed in response to seasonal shifts (P < 0.05). During the dry season, Proteobacteria and Actinobacteria were predominant, while Bacteroidetes and Firmicutes were abundant post-monsoon (P < 0.05). The microbiome harbors potential for the biosynthesis of streptomycin, phenylpropanoid, penicillin, and cephalosporins. Bacteriophages from Podoviridae, Myoviridae, and Siphoviridae showed lytic potential against putrefying and pathogenic bacteria. This first comprehensive study on the glacier-fed Ganges River highlights significant seasonal shifts in microbial diversity. The initial insights into the functional profile of the bacterial and phage diversity offer opportunities to explore various natural compounds and enzymes to tackle antimicrobial resistance under the one-health canopy.

10aBacteriophages10aglacier-fed-Ganges10aMicrobiome10aSecondary metabolites10aspecial properties1 aSamson, Rachel1 aKumar, Shubham1 aDastager, Syed1 aKhairnar, Krishna1 aDharne, Mahesh uhttps://library.ncl.res.in/content/deciphering-comprehensive-microbiome-glacier-fed-ganges-and-functional-aspects-implications