01888nas a2200181   4500008004100000245014700041210006900188260000800257300002500265490000600290520116900296100001801465700002201483700002201505700002501527700002401552856013001576       2017                        eng d00aHigh-throughput mass spectrometry analysis revealed a role for glucosamine in potentiating recovery following desiccation stress in chironomus0 aHighthroughput mass spectrometry analysis revealed a role for gl  cJUN  aArticle Number: 36590 v73 aDesiccation tolerance is an essential survival trait, especially in tropical aquatic organisms that are vulnerable to severe challenges posed by hydroperiodicity patterns in their habitats, characterized by dehydration-rehydration cycles. Here, we report a novel role for glucosamine as a desiccation stress-responsive metabolite in the underexplored tropical aquatic midge, Chironomus ramosus. Using high-throughput liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QToF-MS) analysis, biochemical assays and gene expression studies, we confirmed that glucosamine was essential during the recovery phase in C. ramosus larvae. Additionally, we demonstrated that trehalose, a known stress-protectant was crucial during desiccation but did not offer any advantage to the larvae during recovery. Based on our findings, we emphasise on the collaborative interplay of glucosamine and trehalose in conferring overall resilience to desiccation stress and propose the involvement of the trehalose-chitin metabolic interface in insects as one of the stress-management strategies to potentiate recovery post desiccation through recruitment of glucosamine.1 aThorat, Leena1 aOulkar, Dasharath1 aBanerjee, Kaushik1 aGaikwad, Sushama, M.1 aNath, Bimalendu, B.  uhttps://library.ncl.res.in/content/high-throughput-mass-spectrometry-analysis-revealed-role-glucosamine-potentiating-recovery