01997nas a2200205   4500008004100000245016000041210006900201260000800270300001200278490000800290520120200298100001901500700001701519700002401536700002501560700002601585700002201611700003101633856012701664       2017                        eng d00aDetermination of triazines and triazoles in grapes using atmospheric pressure matrix-assisted laser desorption/ionization high-resolution mass spectrometry0 aDetermination of triazines and triazoles in grapes using atmosph  cMAY  a640-6460 v1003 aA chromatography-free atmospheric pressure matrix-assisted laser desorption/ionization high-resolution mass spectrometry (AP-MALDI HRMS) method is described for the simultaneous and quantitative detection of triazines and triazoles in grapes. The analytes were detected reproducibly with high mass accuracy (mass error within 5 ppm) and further confirmed by collision-induced dissociation fragmentation in tandem MS. The LODs and LOQs for all the analytes were found to be in the nanogram per gram level (15-20 ng/g LOQ). Internal standard normalized high-resolution accurate mass extracted (HR-AM) peak intensities of the detected ions were used to generate the concentration response curves. Linearity (with R-2 values around 0.99) was obtained for these curves within a concentration range of 20-200 ng/g of the individual analytes. The accuracy and precision of the method were further established using QC samples. Validation and performance comparison of the AP-MALDI HRMS method with an existing standard method using LC with :triple quadrupole MS was carried out (evaluating sensitivity, accuracy, precision, and analysis time) using 20 table-grape field samples after QuEChERS extraction.1 aMahale, Vishal1 aSingh, Ajeet1 aPhadke, Gayatri, S.1 aGhanate, Avinash, D.1 aOulkar, Dasharath, P.1 aBanerjee, Kaushik1 aPanchagnula, Venkateswarlu  uhttps://library.ncl.res.in/content/determination-triazines-and-triazoles-grapes-using-atmospheric-pressure-matrix-assisted01888nas a2200181   4500008004100000245014700041210006900188260000800257300002500265490000600290520116900296100001801465700002201483700002201505700002501527700002401552856013001576       2017                        eng d00aHigh-throughput mass spectrometry analysis revealed a role for glucosamine in potentiating recovery following desiccation stress in chironomus0 aHighthroughput mass spectrometry analysis revealed a role for gl  cJUN  aArticle Number: 36590 v73 aDesiccation tolerance is an essential survival trait, especially in tropical aquatic organisms that are vulnerable to severe challenges posed by hydroperiodicity patterns in their habitats, characterized by dehydration-rehydration cycles. Here, we report a novel role for glucosamine as a desiccation stress-responsive metabolite in the underexplored tropical aquatic midge, Chironomus ramosus. Using high-throughput liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QToF-MS) analysis, biochemical assays and gene expression studies, we confirmed that glucosamine was essential during the recovery phase in C. ramosus larvae. Additionally, we demonstrated that trehalose, a known stress-protectant was crucial during desiccation but did not offer any advantage to the larvae during recovery. Based on our findings, we emphasise on the collaborative interplay of glucosamine and trehalose in conferring overall resilience to desiccation stress and propose the involvement of the trehalose-chitin metabolic interface in insects as one of the stress-management strategies to potentiate recovery post desiccation through recruitment of glucosamine.1 aThorat, Leena1 aOulkar, Dasharath1 aBanerjee, Kaushik1 aGaikwad, Sushama, M.1 aNath, Bimalendu, B.  uhttps://library.ncl.res.in/content/high-throughput-mass-spectrometry-analysis-revealed-role-glucosamine-potentiating-recovery