02261nas a2200241   4500008004100000245014200041210006900183260000800252300001400260490000700274520140700281100001601688700001601704700001901720700002201739700002401761700002001785700002301805700002001828700002501848700001701873856012901890       2017                        eng d00aPhoto-induced cytotoxicity and anti-metastatic activity of ruthenium(II)-polypyridyl complexes functionalized with tyrosine or tryptophan0 aPhotoinduced cytotoxicity and antimetastatic activity of rutheni  cMAY  a6634-66440 v463 aThe synergistic effect of oxygen, light, and photosensitizer (PS) has found applications in medicine for the treatment of cancer through photodynamic therapy (PDT). Induction of apoptosis to cancerous cells will prevent tumor metastasis that spreads cancer cells to the neighboring organs/tissues. Herein, we report the two apoptotic Ru(II)-polypyridyl complexes that are functionalized with pendant amino acid moieties tyrosine (1) and tryptophan (2), respectively. These two water soluble complexes were found to interact strongly (K-a(1) = (1.18 +/- 0.28) x 10(5) M-1 and K-a(2) = (1.57 +/- 0.77) x 10(5) M-1) with CT-DNA. Isothermal titration calorimetry (ITC) studies revealed that these complexes bind to CT-DNA through an entropically driven process. Both the complexes showed photo-induced cytotoxicity and exhibit apoptotic activity under photo-irradiation conditions. The comet assay indicated that these complexes can damage cellular DNA, which is attributed to the significant build-up of O-1(2) level even on irradiation with low intensity light (10 J cm(-2), lambda(Range) 450-480 nm). This photoinduced DNA damage and apoptosis in A549 cells was induced by reactive oxygen species (ROS) and occurred through up-regulation of apoptotic marker caspase-3. Control experiments under dark conditions revealed an insignificant cytotoxicity towards these cells for two photosensitive molecules.1 aRamu, Vadde1 aAute, Sunil1 aTaye, Nandaraj1 aGuha, Rweetuparna1 aWalker, Michael, G.1 aMogare, Devaraj1 aParulekar, Apoorva1 aThomas, Jim, A.1 aChattopadhyay, Samit1 aDas, Amitava  uhttps://library.ncl.res.in/content/photo-induced-cytotoxicity-and-anti-metastatic-activity-rutheniumii-polypyridyl-complexes01610nas a2200193   4500008004100000022001400041245008600055210006900141260000900210300001400219490000700233520095900240100002401199700001601223700003001239700001701269700002001286856011001306       2017                        eng d  a1477-922600aRatiometric sensor for DNA based on a dual emission Ru(dppz) light-switch complex0 aRatiometric sensor for DNA based on a dual emission Rudppz light  cMAY   a6079-60860 v463 a<p>Herein we describe the DNA binding properties of two new water-soluble ruthenium complexes; experimental and computational data reveal that both complexes display dual emission from MLCT and LLCT excited states. The interaction of the new complexes with DNA was also investigated. Although one of the complexes only binds DNA though groove binding, the second complex has separate ligands capable of groove binding and intercalation. Nevertheless, it was found that both complexes interact with duplex DNA with high affinity. DNA induced distinctive changes in the emission of the complexes; although the groove binding complex only displays a modest increase in emission on binding, the complex that contains the intercalating Ru-II(dppz) moiety displays a large increase in MLCT-based emission on DNA binding while emission from the LLCT excited state is unaffected. This means that the complex functions as the first ratiometric sensor for DNA.</p>1 aWalker, Michael, G.1 aRamu, Vadde1 aMeijer, Anthony, J. H. M.1 aDas, Amitava1 aThomas, Jim, A.  uhttps://library.ncl.res.in/content/ratiometric-sensor-dna-based-dual-emission-rudppz-light-switch-complex