02402nas a2200265   4500008004100000022001400041245011800055210006900173260000800242300001200250490000700262520150500269653002001774653001501794653001401809653001901823653002501842653002901867653002601896653001601922653002701938100002101965700002601986856012402012       2019                        eng d  a0233-111X00aPuzzle of highly virulent Metarhizium anisopliae strains from Annona squamosa fields against Helicoverpa armigera0 aPuzzle of highly virulent Metarhizium anisopliae strains from An  cAPR  a392-4010 v593 a<p>In our search for indigenous virulent strains of the entomopathogenic fungi, we observed that Metarhizium isolates from soils associated with Annona squamosa (custard apple) have higher virulence (&gt;90% mortality of Helicoverpa armigera larvae at 1/10(th) spore concentration) than strains isolated from Solanum lycopersicum (tomato) fields. Proteomic analysis revealed two insecticidal cyclopeptides of A. squamosa origin in the M. anisopliae strains that led to higher virulence against H. armigera. Transcriptomic and genomic data indicated that M. anisopliae strains and A. squamosa had more than 20 genes in common, including those for cyclic hexapeptide synthase, non-ribosomal peptide synthetase, and plant cyclotide genes, which are involved in the biosynthesis of insecticidal cyclopeptides. These genes were absent in M. anisopliae strains isolated from the S. lycopersicum fields. Further, these strains can establish an endophytic relationship with A. squamosa suggesting that these rhizospheric strains originally could be endophytes, which were eventually released into the soil. Further, Metarhizium strains associated with Capsicum annuum (chili), Azadirachta indica (neem), and Carica papaya (papaya) - plants with insecticidal properties - also had higher virulence against H. armigera. Thus exploration of rhizospheres of plants producing insecticidal metabolites to isolate entomopathogenic fungi, per se, could be a viable strategy in agricultural for crop protection.</p>
10aAnnona squamosa10aBiocontrol10aendophyte10aentomopathogen10aHelicoverpa armigera10ahorizontal gene transfer10ainsecticidal peptides10aMetarhizium10anatural transformation1 aPathan, Ejaj, K.1 aDeshpande, Mukund, V.  uhttps://library.ncl.res.in/content/puzzle-highly-virulent-metarhizium-anisopliae-strains-annona-squamosa-fields-against02086nas a2200265   4500008004100000022001400041245012300055210006900178260000800247300000700255490000700262520120300269653001501472653002201487653002101509653001801530653001901548100002201567700001501589700002301604700002101627700001901648700002601667856012701693       2024                        eng d  a0334-212300aNew record of larval-pupal endoparasitoid Exorista deligata parasitising Indian Tea looper Hyposidra talaca from India0 aNew record of larvalpupal endoparasitoid Exorista deligata paras  cMAR  a230 v523 a<p>
	This is the first record of Exorista deligata (Pandell &amp; eacute;, 1896) (Diptera: Tachinidae) as a parasitoid of Hyposidra talaca (Walker, 1860) (Lepidoptera: Geometridae) from India. H. talaca is a major and new emerging defoliator of the Tea plant Camellia sinensis (L) Kuntze (Theaceae). It completes multiple generations per year without diapause on C. sinensis, resulting in heavy crop loss. The field-collected larvae/pupa of H. talaca were checked for parasitoid infestations and reared until either the host or parasitoid emerged. The parasitoid was identified as E. deligata with morphological characteristics and confirmed by the mitochondrial cytochrome oxidase subunit-I (COX-CO1) gene sequencing technique. The parasitoid is infrequent with unexplored parasitic biology. It parasitises the larva and completes its lifecycle inside the developing H. talaca by entirely devouring the host. The mean percent parasitisation caused by E. deligata studied herein was 25.4% (range 5.88%-57.69%). Based on its parasitisation ability, we suggest the species could be a potential biological control agent to address the damage mitigation caused by the tea looper pest, H. talaca.</p>
10aBiocontrol10aExorista deligata10aHyposidra talaca10aInvasive pest10aParasitisation1 aSharma, Pooja, V.1 aDas, Tamal1 aMukherjee, Susmita1 aPathan, Ejaj, K.1 aRahman, Azizur1 aGathalkar, Ganesh, B.  uhttps://library.ncl.res.in/content/new-record-larval-pupal-endoparasitoid-exorista-deligata-parasitising-indian-tea-looper