02238nas a2200169   4500008004100000245007000041210006900111260000800180300001200188490000700200520167100207100001701878700002101895700002701916700002601943856009901969       2018                        eng d00aAnalysis of tail-anchored protein translocation pathway in plants0 aAnalysis of tailanchored protein translocation pathway in plants  cJUL  a161-1670 v143 aTail-anchored (TA) proteins are a special class of membrane proteins that carry out vital functions in all living cells. Targeting mechanisms of TA proteins are investigated as the best example for post-translational protein targeting in yeast. Of the several mechanisms, Guided Entry of Tail-anchored protein (GET) pathway plays a major role in TA protein targeting. Many in silico and in vivo analyses are geared to identify TA proteins and their targeting mechanisms in different systems including Arabidopsis thaliana. Yet, crop plants that grow in specific and/or different conditions are not investigated for the presence of TA proteins and GET pathway. This study majorly investigates GET pathway in two crop plants, Oryza sativa subsp. Indica and Solanum tuberosum, through detailed in silico analysis. 508 and 912 TA proteins are identified in Oryza sativa subsp. Indica and Solanum tuberosum respectively and their localization with respect to endoplasmic reticulum (ER), mitochondria, and chloroplast has been delineated. Similarly, the associated GET proteins are identified (Get1, Get3 and Get4) and their structural inferences are elucidated using homology modelling. Get3 models are based on yeast Get3. The cytoplasmic Get3 from O. sativa is identified to be very similar to yeast Get3 with conserved P-loop and TA binding groove. Three cytoplasmic Get3s are identified for S. tuberosum. Taken together, this is the first study to identify TA proteins and GET components in Oryza sativa subsp. Indica and Solanum tuberosum, forming the basis for any further experimental characterization of TA targeting and GET pathway mechanisms in crop plants.

1 aManu, M., S.1 aGhosh, Deepanjan1 aChaudhari, Bhushan, P.1 aRamasamy, Sureshkumar  uhttps://library.ncl.res.in/content/analysis-tail-anchored-protein-translocation-pathway-plants03002nas a2200265   4500008004100000022001400041245014400055210006900199260000800268300001400276490000800290520209300298653001502391653000802406653003602414653001202450653001902462653001502481653002302496100001802519700001802537700002702555700002302582856013102605       2023                        eng d  a0022-354900aAptamer tethered bio-responsive mesoporous silica nanoparticles for efficient targeted delivery of paclitaxel to treat ovarian cancer cells0 aAptamer tethered bioresponsive mesoporous silica nanoparticles f  cMAY  a1450-14590 v1123 a<p>
	Ovarian cancer is the leading cause of cancer deaths in female patients. The current therapeutics in ovarian cancer are limited and inefficient in curing the disease. To tackle this, we have synthesized tetrasulfide deriv-ative of silica doped, biodegradable, glutathione-responsive targeted mesoporous silica nanoparticles modi-fied with heterobifunctional polyethylene glycol as a linker and mucin-1 aptamer for triggered paclitaxel delivery to the ovarian cancer cells. Degradable mesoporous silica nanoparticles were synthesized by a modi-fied sol-gel method with tetraethyl orthosilicate and Bis (triethoxysilylpropyl) tetrasulfide. The degradable mesoporous silica nanoparticles were characterized by dynamic light scattering, Fourier-transform infrared spectroscopy, Scanning electron microscopy and Transmission electron microscopy. The degradable mesopo-rous silica nanoparticles had good paclitaxel encapsulation efficiency and glutathione-responsive paclitaxel release ability. The glutathione utilization assay and visual destruction observed within 10 days in transmis-sion electron microscopy images confirmed the degradation of the mesoporous silica nanoparticles in the tumor cell environment. The targeted degradable mesoporous silica nanoparticles were efficiently taken up by ovarian cancer cell lines OVACAR-3 and PA-1. The cytotoxicity of bare mesoporous silica nanoparticles evaluated on NIH-3T3 cell line showed good biocompatibility (&gt;90% cell viability). Significant toxicity on OVACAR-3 (IC50 25.66 nM) and PA-1 (IC50 42.93 nM) cell lines was observed when treated with paclitaxel-loaded targeted degradable mesoporous silica nanoparticles. Results of this study demonstrated that mucin-1 targeted, glutathione-responsive mesoporous silica nanoparticles loaded with paclitaxel had a significant antitumor effect on ovarian cancer cells. All these findings demonstrated that developed nano-formulation could be suitable for ovarian cancer treatment. &amp; COPY; 2023 American Pharmacists Association. Published by Elsevier Inc. All rights reserved.</p>
10aDegradable10aGSH10aMesoporous silica nanoparticles10aMucin-110aOvarian cancer10aPaclitaxel10aStimuli-responsive1 aSalve, Rajesh1 aKumar, Pramod1 aChaudhari, Bhushan, P.1 aGajbhiye, Virendra  uhttps://library.ncl.res.in/content/aptamer-tethered-bio-responsive-mesoporous-silica-nanoparticles-efficient-targeted-delivery