02864nas a2200229 4500008004100000245010200041210006900143260000800212300001600220490000600236520208600242100001802328700001702346700001402363700001902377700002102396700002402417700002702441700002302468700001702491856012602508 2016 eng d00aBilayer cryogel wound dressing and skin regeneration grafts for the treatment of acute skinwounds0 aBilayer cryogel wound dressing and skin regeneration grafts for  cJUN a15145-151590 v83 aIn this study, the potential of cryogel bilayer wound dressing and skin regenerating graft for the treatment of surgically created full thickness wounds was evaluated. The top layer was composed of polyvinylpyrrolidone-iodine (PVP-I) and served as the antiseptic layer, while the bottom regenerative layer was made using gelatin cryogel. Both components of the bilayer showed typical features of a cryogel interconnected macropore network, rapid swelling, high water uptake capacity of about 90%. Both PVP and gelatin cryogel showed high tensile strength of 45 and 10 kPa, respectively. Gelatin cryogel sheets were essentially elastic and could be stretched without any visible deformation. The antiseptic PVP-I layer cryogel sheet showed sustained iodine release and suppressed microbial growth when tested with skin pathogens (zone of inhibition cm for sheet of 0.9 cm diameter). The gelatin cryogel sheet degraded in vitro in weeks. The gelatin cryogel sheet supported cell infiltration, attachment, and proliferation of fibroblasts and keratinocytes. Microparticles loaded with bioactive molecules (mannose-6-phosphate and human fibrinogen) were also incorporated in the gelatin cryogel sheets for their role in enhancing skin regeneration and scar free wound healing. In vivo evaluation of healing capacity of the bilayer cryogel was checked in rabbits by creating full thickness wound, defect (diameter 2 cm). Macroscopic and microscopic observation at regular time intervals for 4 weeks demonstrated better and faster skin regeneration in the wound treated with cryogel bilayer as compared to untreated defect and the repair was comparable to commercial skin regeneration scaffold Neuskin-F. Complete skin regeneration was observed after 4 weeks' of implantation with no sign of inflammatory response. Defects implanted with cryogel having mannose-6-phosphate showed no scar formation,, while the wound treated with bilayer incorporated with human fibrinogen microparticles showed early signs of skin regeneration; epidermis formation occurred at 2 weeks after implantation.1 aPriya, Geetha1 aGupta, Ankur1 aJain, Era1 aSarkar, Joyita1 aDamania, Apeksha1 aJagdale, Pankaj, R.1 aChaudhari, Bhushan, P.1 aGupta, Kailash, C.1 aKumar, Ashok uhttps://library.ncl.res.in/content/bilayer-cryogel-wound-dressing-and-skin-regeneration-grafts-treatment-acute-skinwounds02652nas a2200241 4500008004100000245016400041210006900205260000800274300001200282490000700294520177000301100002602071700001902097700001702116700002102133700001502154700002302169700002702192700001502219700002502234700002702259856012402286 2016 eng d00aCutaneous exposure to clinically-relevant pigeon pea (Cajanus cajan) proteins promote T(H)2-dependent sensitization and IgE-mediated anaphylaxis in Balb/c mice0 aCutaneous exposure to clinicallyrelevant pigeon pea Cajanus caja cJAN a827-8410 v133 aEpicutaneous (EC) sensitization to food allergens may occur when the skin has been lightly damaged. The study here tested whether cutaneous exposure to pigeon pea protein(s) may cause allergic sensitization. BALB/c mice were either orally gavaged or epicutaneously sensitized by repeated application of pigeon pea crude protein extract (CPE) on undamaged areas of skin without any adjuvant; afterwards, both groups were orally challenged with the pigeon pea CPE. Anaphylactic symptoms along with measures of body temperature, MCPT-1, TSLP, pigeon pea-specific IgE and IgG(1), myeloperoxidase (MPO) activity, T(H)2 cytokines, T(H)2 transcription factors (TFs) and filaggrin expression were determined. Mast cell staining, eosinophil levels and histopathological analysis of the skin and intestines were also performed. In the epicutaneously-sensitized mice, elevated levels of specific IgE and IgG(1), as well as of MCPT-1, TSLP, T(H)2 cytokines and TFs, higher anaphylactic scores and histological changes in the skin and intestine were indicative of sensitization ability via both routes in the pigeon pea CPE-treated hosts. Elevated levels of mast cells were observed in both the skin and intestine; increased levels of eosinophils and MPO activity were noted only in the skin. Decreased levels of filaggrin in skin may have played a key role in the skin barrier dysfunction, increasing the chances of sensitization. Therefore, the experimental data support the hypothesis that in addition to oral exposure, skin exposure to food allergens can promote T(H)2-dependent sensitization, IgE-mediated anaphylaxis and intestinal changes after oral challenge. Based on this, an avoidance of cutaneous exposures to allergens might prevent development of food anaphylaxis.1 aGupta, Rinkesh, Kumar1 aKumar, Sandeep1 aGupta, Kriti1 aSharma, Akanksha1 aRoy, Ruchi1 aVerma, Alok, Kumar1 aChaudhari, Bhushan, P.1 aDas, Mukul1 aAnsari, Irfan, Ahmad1 aDwivedi, Premendra, D. uhttps://library.ncl.res.in/content/cutaneous-exposure-clinically-relevant-pigeon-pea-cajanus-cajan-proteins-promote-th201351nas a2200205 4500008004100000245010200041210006900143260000800212300001200220490000800232520061900240100002900859700002000888700002000908700002800928700002100956700001900977700002700996856012201023 2017 eng d00aRapid synthesis of highly monodispersed silver nanoparticles from the leaves of Salvadora persica0 aRapid synthesis of highly monodispersed silver nanoparticles fro cOCT a226-2290 v2053 aWe have demonstrated a one pot green protocol for the synthesis of highly monodispersed silver nanoparticles using leaves of Salvadora persica plant. When the leaf extract of Salvadora persica plant reacted with silver nitrate (AgNO3) solution it resulted in the synthesis of highly monodispersed silver nanoparticles with an average size of 3 nm. These nanoparticles were then completely characterized by UV-Visible spectroscopy, Transmission Electron Microscopy, Energy Dispersive Analysis of X-Rays, X-ray diffraction analysis and Fourier Transform Infrared analysis. (C) 2017 Elsevier B.V. All rights reserved.1 aKhan, Mamatha, R. Shadab1 aSalunkhe, Pooja1 aSatpute, Shruti1 aKendurkar, Shuchishweta1 aPrabhune, Asmita1 aDeval, Animesh1 aChaudhari, Bhushan, P. uhttps://library.ncl.res.in/content/rapid-synthesis-highly-monodispersed-silver-nanoparticles-leaves-salvadora-persica02238nas a2200169 4500008004100000245007000041210006900111260000800180300001200188490000700200520167100207100001701878700002101895700002701916700002601943856009901969 2018 eng d00aAnalysis of tail-anchored protein translocation pathway in plants0 aAnalysis of tailanchored protein translocation pathway in plants cJUL a161-1670 v143 aTail-anchored (TA) proteins are a special class of membrane proteins that carry out vital functions in all living cells. Targeting mechanisms of TA proteins are investigated as the best example for post-translational protein targeting in yeast. Of the several mechanisms, Guided Entry of Tail-anchored protein (GET) pathway plays a major role in TA protein targeting. Many in silico and in vivo analyses are geared to identify TA proteins and their targeting mechanisms in different systems including Arabidopsis thaliana. Yet, crop plants that grow in specific and/or different conditions are not investigated for the presence of TA proteins and GET pathway. This study majorly investigates GET pathway in two crop plants, Oryza sativa subsp. Indica and Solanum tuberosum, through detailed in silico analysis. 508 and 912 TA proteins are identified in Oryza sativa subsp. Indica and Solanum tuberosum respectively and their localization with respect to endoplasmic reticulum (ER), mitochondria, and chloroplast has been delineated. Similarly, the associated GET proteins are identified (Get1, Get3 and Get4) and their structural inferences are elucidated using homology modelling. Get3 models are based on yeast Get3. The cytoplasmic Get3 from O. sativa is identified to be very similar to yeast Get3 with conserved P-loop and TA binding groove. Three cytoplasmic Get3s are identified for S. tuberosum. Taken together, this is the first study to identify TA proteins and GET components in Oryza sativa subsp. Indica and Solanum tuberosum, forming the basis for any further experimental characterization of TA targeting and GET pathway mechanisms in crop plants. 1 aManu, M., S.1 aGhosh, Deepanjan1 aChaudhari, Bhushan, P.1 aRamasamy, Sureshkumar uhttps://library.ncl.res.in/content/analysis-tail-anchored-protein-translocation-pathway-plants01977nas a2200133 4500008004100000245008500041210006900126260001300195300001200208520146600220100002101686700002701707856010901734 2019 eng d00aFungi the crucial contributors for nanotechnology: a green chemistry perspective0 aFungi the crucial contributors for nanotechnology a green chemis bSpringer a279-2983 aMyconanotechnology has gained an exceptional momentum in today’s world. It encompasses a wide range of applications like in agriculture, biomedical, electronics, textiles, cell and molecular biology, nanodevices, and many more. This fast-growing research field has a list of many nanoparticles that have been synthesized from different kinds of fungi. It is an eco-friendly approach that has sidelined the conventional methods of chemical and physical synthesis that require high energy and are toxic and expensive. The fungal-based synthesis gains functionality due to its discrete and diversified advantages that are being explored. Various parameters like temperature, pH, time and concentrations of fungal biomass or extract as well as of the precursor play a vital role in the protocols. These factors have to be optimized for the laboratory as well as industrial scale production. A greater importance to the use of cell-free extracts for nanosynthesis has been given. They contain the enzymes that are reported to be involved in the synthesis mechanisms as reducing agents and capping agents. The role of fungi in nanosynthesis and nanotechnology per se has many patents under its name. Although there are voids in the existing synthesis methods as well as lacunae in the application part that need to be addressed and filled. With an extensive research and work, myconanoparticles can be exploited to the maximum and lead its advancement in new areas.1 aMoudgil, Aliesha1 aChaudhari, Bhushan, P. uhttps://library.ncl.res.in/content/fungi-crucial-contributors-nanotechnology-green-chemistry-perspective02428nas a2200241 4500008004100000022001400041245015600055210006900211260000800280300000900288490000700297520153600304100002101840700002301861700001901884700002701903700002601930700002001956700002701976700002802003700002802031856012702059 2020 eng d a2045-232200aHighly sensitive label-free bio-interfacial colorimetric sensor based on silk fibroin-gold nanocomposite for facile detection of chlorpyrifos pesticide0 aHighly sensitive labelfree biointerfacial colorimetric sensor ba cMAR a41980 v103 a

Herein, the preparation of gold nanoparticles-silk fibroin (SF-AuNPs) dispersion and its label-free colorimetric detection of the organophosphate pesticide, namely chlorpyrifos, at ppb level are reported. The silk fibroin solution was extracted from B. mori silk after performing degumming, dissolving and dialysis steps. This fibroin solution was used for synthesis of gold nanoparticles in-situ without using any external reducing and capping agent. X-ray Diffractometry (XRD), Field Emission Transmission Electron Microscopy (FETEM) along with Surface Plasmon Resonance based optical evaluation confirmed generation of gold nanoparticles within SF matrix. The resultant SF-AuNPs dispersion exhibited rapid and excellent colorimetric pesticide sensing response even at 10 ppb concentration. Effect of additional parameters viz. pH, ionic concentration and interference from other pesticide samples was also studied. Notably, SF-AuNPs dispersion exhibited selective colorimetric pesticide sensing response which can be calibrated. Furthermore, this method was extended to various simulated real life samples such as tap water, soil and agricultural products including plant residues to successfully detect the presence of chlorpyrifos pesticide. The proposed colorimetric sensor system is facile yet effective and can be employed by novice rural population and expert researchers alike. It can be exploited as preliminary tool for label-free colorimetric chlorpyrifos pesticide sensing in water and agricultural products.

1 aMane, Pramod, C.1 aShinde, Manish, D.1 aVarma, Sanjana1 aChaudhari, Bhushan, P.1 aFatehmulla, Amanullah1 aShahabuddin, M.1 aAmalnerkar, Dinesh, P.1 aAldhafiri, Abdullah, M.1 aChaudhari, Ravindra, D. uhttps://library.ncl.res.in/content/highly-sensitive-label-free-bio-interfacial-colorimetric-sensor-based-silk-fibroin-gold02475nas a2200265 4500008004100000022001400041245013200055210006900187260000900256300001100265490000800276520158300284653002401867653000801891653001301899653001801912653001001930653002301940100002501963700002701988700002602015700001902041700002302060856012602083 2020 eng d a0378-517300aStimuli-responsive biodegradable polyurethane nano-constructs as a potential triggered drug delivery vehicle for cancer therapy0 aStimuliresponsive biodegradable polyurethane nanoconstructs as a cOCT  a1197810 v5883 a

Polyurethanes (PUs) constitute an essential class of stimuli-responsive and biodegradable material, which has significantly contributed to the advancement of polymers utilization in the biomedical field. The bio-erodible PUs construct an active corridor for facilitating drug into tumor cells, which has significantly impacted the progression of nano-micellar delivery systems. The self-assembled colloidal PUs pose distinctive features such as enhancing the solubility of hydrophobic chemotherapeutics, rapid cellular uptake, triggered erosion and drug release, bio-stimulus sensitivity, improvement in the targeting and proficiency of bioactive. Cationic PUs can easily be condensed with genetic material to form polyplexes and have shown excellent transfection efficiency for potential gene therapy against various cancers. Their modifiable chemistry offers a tool to impart the desired multifunctionality such as biocompatibility, sensitivity to pH, redox, temperature, enzyme, etc. and ligand conjugation for active targeting. These diverse exceptional properties make them excellent nano-carrier for a variety of bioactive, including chemotherapeutic drugs, DNA, RNA, and diagnostic moieties to the target tissue or cells. The PUs based nano-devices have certainly uncovered the path to achieve ideal systems for controlled personalized therapy. The literature discussed in this review shed light on the research innovations carried out in the last ten years for the development of multifunctional PUs for triggered delivery of bioactive to treat various cancers.

10aCancer nanomedicine10aGSH10amicelles10aPolyurathanes10aredox10aStimuli-responsive1 aGajbhiye, Kavita, R.1 aChaudhari, Bhushan, P.1 aPokharkar, Varsha, B.1 aPawar, Atmaram1 aGajbhiye, Virendra uhttps://library.ncl.res.in/content/stimuli-responsive-biodegradable-polyurethane-nano-constructs-potential-triggered-drug02322nas a2200241 4500008004100000022001400041245014000055210006900195260000800264300001100272490000700283520143600290653002701726653002901753653002001782653002301802653002701825653002701852100001801879700002801897700002701925856012801952 2020 eng d a1773-224700aUnderstanding fundamentals of hepatocellular carcinoma to design next-generation chitosan nano-formulations: Beyond chemotherapy stride0 aUnderstanding fundamentals of hepatocellular carcinoma to design cAUG a1017230 v583 a

Hepatocellular carcinoma (HCC) is the deadliest form of liver cancer. Clinically, the main strategies currently being used for the treatment of HCC are surgery, radiotherapy and chemotherapy. Conventional chemotherapy has major drawbacks such as poor bioavailability, high-dose requirements, adverse side effects, low therapeutic indices, and non-specific drug targeting. Therefore, targeted drug delivery systems are fast becoming new tools for the selective killing of cancer cells. Chitosan (CS) is a biodegradable, biocompatible, cationic and natural biopolymer that also exhibits anti-cancer property which is now being explored as a promising candidate for targeted drug delivery. This review outlines an overview of the causative agents, microenvironment, pathophysiology, surface-biomarkers and physiological barriers of HCC. Then, the cellular internalization pathways of nanomedicine and the important physicochemical properties of delivery agents are discussed. The benefits of targeted therapy over conventional therapy with regard to HCC are also discussed. The main objective of this review was to summarize the current knowledge in the field of chitosan-based drug delivery for the management of HCC along with its limitations in a comprehensive and systematic way. This review attempts to provide a holistic roadmap for designing the next-generation chitosan-based drug delivery systems for HCC management.

10achitosan nanoparticles10aHepatocellular carcinoma10aPathophysiology10aSurface biomarkers10aTargeted Drug Delivery10aTumor microenvironment1 aJaiswal, Neha1 aChaudhari, Ravindra, D.1 aChaudhari, Bhushan, P. uhttps://library.ncl.res.in/content/understanding-fundamentals-hepatocellular-carcinoma-design-next-generation-chitosan-nano02413nas a2200277 4500008004100000022001400041245014600055210006900201260000800270300001200278490000700290520142000297653002701717653002501744653002401769653002501793653002501818653002501843100002101868700002301889700002301912700002301935700002401958700002701982856012602009 2021 eng d a1040-727800aEichhornia crassipes mediated bioinspired synthesis of crystalline nano silver as an integrated medicinal material: a waste to value approach0 aEichhornia crassipes mediated bioinspired synthesis of crystalli cMAR a391-4040 v323 a

The present study deals with the synthesis of silver nanoparticles from Eichhornia crassipes. Dynamic light scattering (DLS), Transmission electron microscopy (TEM), UV-Vis spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy and X-ray diffraction (XRD) were performed for nanoparticle characterization. The aqueous plant extract of Eichhornia crassipes was phytochemically analyzed for phenol, flavonoid, reducing sugar content, alkaloids, saponins and carotenoids. The synthesized particles after optimization of important parameters have an average diameter of 12.48 +/- 3.43 nm with a spherical morphology and zeta potential of - 31.53 mV. At 100 mu g/ml of nanoparticle concentration, the antioxidant activity of 93.6% was observed. MIC (Minimum inhibitory concentration) values exhibiting the antimicrobial attributes reported an estimated value of 7.8 mu g/ml for gram-negative and higher values of 31.25 and 250 mu g/ml for gram-positive bacteria. The antibiofilm assay showed 86.89% and 74.7% of the reduction in violacein synthesis and biofilm inhibition respectively at 15 mu g/ml nanoparticle concentration. The anticancer assay reported the IC50 (Inhibitory concentration) values of 13.32, 14.71 and 19.91 mu g/ml for HeLa, HCT 116 and L6 cell lines respectively. Thus the study establishes a significant integrative treatment to combat secondary infections in cancer patients. Graphic

10aantibacterial activity10aAntibiofilm activity10aAnticancer activity10aAntioxidant activity10aEichhornia crassipes10asilver nanoparticles1 aMoudgil, Aliesha1 aDeval, Animesh, S.1 aDharne, Mahesh, S.1 aSarkar, Dhiman, M.1 aChoudhari, Amit, S.1 aChaudhari, Bhushan, P. uhttps://library.ncl.res.in/content/eichhornia-crassipes-mediated-bioinspired-synthesis-crystalline-nano-silver-integrated02337nas a2200241 4500008004100000022001400041245015700055210006900212260000900281300001200290490000800302520146900310653001201779653001401791653002901805100001601834700002401850700002801874700001901902700002701921700002101948856012601969 2022 eng d a0141-813000aMechanistic insight into differential interactions of iron oxide nanoparticles with native, glycated albumin and their effect on erythrocytes parameters0 aMechanistic insight into differential interactions of iron oxide cJUL  a232-2470 v2123 a

Nanoparticles and protein bioconjugates have been studied for multiple biomedical applications. We sought to investigate the interaction and structural modifications of bovine serum albumin (BSA) with iron oxide nanoparticles (IONPs). The IONPs were green synthesized using E. crassipes aqueous leaf extract following characterization using transmission electron microscopy, energy dispersive X-ray analysis and X-ray diffraction. Two different concentrations of native/glycated albumin (0.5 and 1.5 mg/ml) with IONPs were allowed to interact for 1 h at 37 degrees C. Glycation markers, protein modification markers, cellular antioxidant, and hemolysis studies showed structural modifications and conformational changes in albumin due to the presence of IONPs. UV-visible absorbance resulted in hyperchromic and bathochromic effects of IONPs-BSA conjugates. Fluorescence measurements of tyrosine, tryptophan, advanced glycated end products, and ANS binding assay were promising and quenching effects proved IONPs-BSA conjugate formation. In FTIR of BSA-IONPs, transmittance was increased in amide A and B bands while decreased in amide I and II bands. In summary, native PAGE, HPLC, and FTIR analysis displayed a differential behaviour of IONPs with native and glycated BSA. These results provided an understanding of the interaction and structural modifications of glycated and native BSA which may provide fundamental repercussions in future studies.

10aAlbumin10aGlycation10airon oxide nanoparticles1 aNisar, Akib1 aAjabia, Devangi, K.1 aAgrawal, Sanskruthi, B.1 aVarma, Sanjana1 aChaudhari, Bhushan, P.1 aTupe, Rashmi, S. uhttps://library.ncl.res.in/content/mechanistic-insight-differential-interactions-iron-oxide-nanoparticles-native-glycated02395nas a2200205 4500008004100000020001400041245019500055210006900250260000800319520162100327100002101948700001901969700002301988700003002011700002302041700002802064700002302092700002702115856004702142 2022 eng d a2522-574X00aOne-pot concurrent biosynthesis of biphasic CuxO (cuprous and cupric oxide) nanoparticles using leaf extract of Eichhornia crassipes and investigation of their potent healthcare applications0 aOnepot concurrent biosynthesis of biphasic CuxO cuprous and cupr cFEB3 aEichhornia crassipes, an aquatic weed, has been used for the one-pot quick synthesis of cuprous and cupric oxide nanoparticles in this report. The identification and validation of the formed nanoparticles were successfully done by sophisticated characterization techniques such as UV–visible spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. Their biomedical interventions were assessed by their antioxidant and anticancer activities. The synthesized nanoparticles have a spherical morphology with an average diameter of 10 nm. Antioxidant assay performed showed an increasing trend in a dose-dependent manner with the percentage radical scavenging activity up to 94.70% at a much higher concentration of 1000 µg/ml. The antioxidant potential at such higher concentration is suspected to invoke a change in the cytotoxic potential of the nanoparticles which is then verified by the MTT assay. A significant cytotoxic activity against HeLa (cervical cancer) and HCT 116 (colorectal carcinoma) cell lines was detected with noted IC50 values of 17.17 and 13.70 µg/ml respectively. The conclusive findings obtained are enough to substantiate the use of these nanoparticles in biomedical areas specifically in anticancer treatment due to their high toxicities. The hazards imposed by Eichhornia crassipes can be alleviated by using them as biofactories for the synthesis of a variety of nanomaterials. This helps in curbing the water pollution issues as well as the developing synthesis protocols for robust and stable nanoparticles.1 aMoudgil, Aliesha1 aVarma, Sanjana1 aShinde, Manish, D.1 aVamkudoth, Koteswara, Rao1 aSarkar, Dhiman, M.1 aShende, Rajnigandha, A.1 aAmalnerkar, Dinesh1 aChaudhari, Bhushan, P. uhttps://doi.org/10.1007/s42247-022-00347-103002nas a2200265 4500008004100000022001400041245014400055210006900199260000800268300001400276490000800290520209300298653001502391653000802406653003602414653001202450653001902462653001502481653002302496100001802519700001802537700002702555700002302582856013102605 2023 eng d a0022-354900aAptamer tethered bio-responsive mesoporous silica nanoparticles for efficient targeted delivery of paclitaxel to treat ovarian cancer cells0 aAptamer tethered bioresponsive mesoporous silica nanoparticles f cMAY a1450-14590 v1123 a

Ovarian cancer is the leading cause of cancer deaths in female patients. The current therapeutics in ovarian cancer are limited and inefficient in curing the disease. To tackle this, we have synthesized tetrasulfide deriv-ative of silica doped, biodegradable, glutathione-responsive targeted mesoporous silica nanoparticles modi-fied with heterobifunctional polyethylene glycol as a linker and mucin-1 aptamer for triggered paclitaxel delivery to the ovarian cancer cells. Degradable mesoporous silica nanoparticles were synthesized by a modi-fied sol-gel method with tetraethyl orthosilicate and Bis (triethoxysilylpropyl) tetrasulfide. The degradable mesoporous silica nanoparticles were characterized by dynamic light scattering, Fourier-transform infrared spectroscopy, Scanning electron microscopy and Transmission electron microscopy. The degradable mesopo-rous silica nanoparticles had good paclitaxel encapsulation efficiency and glutathione-responsive paclitaxel release ability. The glutathione utilization assay and visual destruction observed within 10 days in transmis-sion electron microscopy images confirmed the degradation of the mesoporous silica nanoparticles in the tumor cell environment. The targeted degradable mesoporous silica nanoparticles were efficiently taken up by ovarian cancer cell lines OVACAR-3 and PA-1. The cytotoxicity of bare mesoporous silica nanoparticles evaluated on NIH-3T3 cell line showed good biocompatibility (>90% cell viability). Significant toxicity on OVACAR-3 (IC50 25.66 nM) and PA-1 (IC50 42.93 nM) cell lines was observed when treated with paclitaxel-loaded targeted degradable mesoporous silica nanoparticles. Results of this study demonstrated that mucin-1 targeted, glutathione-responsive mesoporous silica nanoparticles loaded with paclitaxel had a significant antitumor effect on ovarian cancer cells. All these findings demonstrated that developed nano-formulation could be suitable for ovarian cancer treatment. & COPY; 2023 American Pharmacists Association. Published by Elsevier Inc. All rights reserved.

10aDegradable10aGSH10aMesoporous silica nanoparticles10aMucin-110aOvarian cancer10aPaclitaxel10aStimuli-responsive1 aSalve, Rajesh1 aKumar, Pramod1 aChaudhari, Bhushan, P.1 aGajbhiye, Virendra uhttps://library.ncl.res.in/content/aptamer-tethered-bio-responsive-mesoporous-silica-nanoparticles-efficient-targeted-delivery02873nas a2200277 4500008004100000022001400041245013300055210006900188260000800257300001200265490000700277520189600284653003802180653002302218653001702241653002302258653002702281100002102308700001902329700001702348700002502365700001802390700002702408700003002435856013002465 2023 eng d a1139-670900aBiosynthesis of gold nanoparticles by Penicillium rubens and catalytic detoxification of ochratoxin A and organic dye pollutants0 aBiosynthesis of gold nanoparticles by Penicillium rubens and cat cNOV a765-7800 v263 a

The environmental pollution caused by chemical dyes is a growing concern nowadays. Limitations of traditional methods opened the route for nanotechnology; owing to the versatile properties of nanomaterials, gold nanoparticles (AuNPs) became a potential strategy for different applications. In the present study, biosynthesis of gold nanoparticles (BioAuNPs) was carried out by reacting chloroauric acid (HAuCl4) with cell-free filtrate of Penicillium rubens sp. nov. NCIM 1937. The AuNPs were then characterized by UV-visible spectroscopy, HR-TEM, FTIR, and DLS analysis to further examine their efficacious biosynthesis and morphological properties including size, shape, and stability. The biogenic AuNPs are polydisperse in nature, with a mean size of 14.92 +/- 5 nm. These AuNPs exhibited promising antimicrobial activity against Escherichia coli NCIM-2065, Bacillus subtilis NCIM-2010, and Penicillium verrucosum MTCC 4935. In vitro quantitative HPLC results revealed that BioAuNPs significantly inhibited the biosynthesis of ochratoxin A (OTA). Microbial fuel cells (MFCs) are intriguing for power generation and wastewater treatment since they can directly transform chemical energy stored in organic matter to electricity by extracellular electron transfer (EET) via membrane proteins. AuNPs also showed excellent potential for dye degradation of organic pollutants, viz., methylene blue (MB), phenol red (PR), bromothymol blue (BTB), Congo red (CR), and 4-nitrophenol (4-NP). All dye removal efficiencies were estimated and fitted to pseudo-first-order processes using kinetic rate constants (Ka).The present study reveals a simple, original, and eco-friendly method for the synthesis of multifunctional biogenic AuNPs that could be effective in OTA detoxification in food products and organic pollutant removal during wastewater treatment for a sustainable environment.

10aCatalytic organic dye degradation10agold nanoparticles10aOchratoxin A10aPenicillium rubens10aPenicillium verrucosum1 aBhandari, Yogesh1 aVarma, Sanjana1 aSawant, Amol1 aBeemagani, Sreelatha1 aJaiswal, Neha1 aChaudhari, Bhushan, P.1 aVamkudoth, Koteswara, Rao uhttps://library.ncl.res.in/content/biosynthesis-gold-nanoparticles-penicillium-rubens-and-catalytic-detoxification-ochratoxin02536nas a2200229 4500008004100000022001400041245013200055210006900187260000800256300001100264490000800275520170400283653001801987653002802005653002902033653002702062100002102089700001802110700002302128700002702151856012802178 2023 eng d a0009-308400aChallenges and emerging strategies for next generation liposomal based drug delivery: an account of the breast cancer conundrum0 aChallenges and emerging strategies for next generation liposomal cJAN a1052580 v2503 a

The global cancer burden is witnessing an upsurge with breast cancer surpassing other cancers worldwide. Furthermore, an escalation in the breast cancer caseload is also expected in the coming years. The conventional therapeutic regimens practiced routinely are associated with many drawbacks to which nanotechnological in-terventions offer a great advantage. But how eminent could liposomes and their advantages be in superseding these existing therapeutic modalities? A solution is reflected in this review that draws attention to a decade-long journey embarked upon by researchers in this wake. This text is a comprehensive discussion of liposomes, the front runners of the drug delivery systems, and their active and passive targeting approaches for breast cancer management. Active targeting has been studied over the decade by many receptors overexpressed on the breast cancer cells and passive targeting with many drug combinations. The results converge on the fact that the actively targeted formulations exhibit a superior efficacy over their non-targeted counterparts and the all lipo-somal formulations are efficacious over the free drugs. This undoubtedly underlines the dominion of liposomal formulations over conventional chemotherapy. These investigations have led to the development of different liposomal formulations with active and passive targeting capacities that could be explored in depth. Acknowl-edging and getting a deeper insight into the liposomal evolution through time also unveiled many imperfections and unchartered territories that can be explored to deliver dexterous liposomal formulations against breast cancer and more in the clinical trial pipeline.

10aBreast cancer10aLiposomal metamorphosis10aReceptor-ligand dynamics10aTargeted Drug Delivery1 aMoudgil, Aliesha1 aSalve, Rajesh1 aGajbhiye, Virendra1 aChaudhari, Bhushan, P. uhttps://library.ncl.res.in/content/challenges-and-emerging-strategies-next-generation-liposomal-based-drug-delivery-account01851nas a2200217 4500008004100000022001400041245010800055210006900163260000800232300001500240490000600255520105700261653003101318653003501349653002501384653001801409653002201427100002801449700002701477856012901504 2023 eng d a2365-654900aRobust optimization and characterization of MCM-41 nanoparticle synthesis using modified sol-gel method0 aRobust optimization and characterization of MCM41 nanoparticle s cMAR ae2022049680 v83 a

Mesoporous silica nanoparticles (MSN) for drug delivery application requires great expertise to synthesize small sized monodisperse particles. We aimed at synthesizing and optimizing MCM-41 particles with the modified Stober's method by adjusting factors such as concentrations of cetyltrimethylammonium bromide (CTAB), sodium hydroxide (NaOH) and water (H2O) with respect to tetraethyl orthosilicate (TEOS), and a process parameter (stirring). We comprehended that the reactants determined the shape and size of both the particles and pores. Upon extensive characterization we report the synthesis of spherical particles of similar to 70 nm size in a monodisperse population; the structures had hexagonal ordered pores of diameter 3.071 nm and volume 3.124 cm(3)/g; the total surface area of 3410.648 m(2)/g of the particles was covered with silanol groups that aid in functionalization; the particles exhibited great thermal stability. Thus, the extensive optimization fabricated robust MCM-41 particles fit for drug delivery application.

10acharacterization technique10adynamic light scattering (DLS)10amesoporous materials10aNanoparticles10asol gel processes1 aShende, Rajnigandha, A.1 aChaudhari, Bhushan, P. uhttps://library.ncl.res.in/content/robust-optimization-and-characterization-mcm-41-nanoparticle-synthesis-using-modified-sol02106nas a2200205 4500008004100000022001400041245012300055210006900178260000900247490000600256520135300262653001901615653003501634653001801669653001401687653002101701100002101722700002701743856013001770 2023 eng d a2365-654900aUnderstanding critical aspects of liposomal synthesis for designing the next generation targeted drug delivery vehicle0 aUnderstanding critical aspects of liposomal synthesis for design cOCT 0 v83 a

We identified process parameters of the thin film hydration technique and intrinsic factors to synthesize liposomes for drug delivery. The thin film formation step impacted the nature of the lipid layer, and we optimized 240 RPM rotation speed, 700 mm of Hg vacuum pressure, and 2 ml of chloroform as the organic solvent. The hydration step controlled the particle specifications, and we optimized 270 RPM rotation speed, PBS as the hydrating medium, and 1 h hydration time. We obtained a comparatively smaller liposomal population with a lower size distribution just after hydrating the lipid layer that required milder downsizing steps -10 extrusion passes through a single polycarbonate membrane. The intrinsic factors including the concentrations and molar ratio of lipids affected the synthesis steps and the particle specifications. Characterization of liposomes by analytical techniques confirmed the synthesis of a monodisperse population with hydrodynamic diameter<150 nm, moderate stability, spherical morphology, and high thermal and storage stability. This comprehensive study defines the role of every parameter, provides a mechanistic insight into synthesis that is supported by experimental data, and helps tune specific parameters to synthesize liposomes for drug delivery or any application with desired specifications.

10acarrier system10adesigning and characterization10adrug delivery10aliposomes10atargeting moiety1 aMoudgil, Aliesha1 aChaudhari, Bhushan, P. uhttps://library.ncl.res.in/content/understanding-critical-aspects-liposomal-synthesis-designing-next-generation-targeted-drug02483nas a2200133 4500008004100000245012900041210006900170260000800239520190900247100001802156700002002174700002702194856012802221 2024 eng d00aChitosan nanoparticles for single and combinatorial delivery of 5-fluorouracil and ursolic acid for hepatocellular carcinoma0 aChitosan nanoparticles for single and combinatorial delivery of  cOCT3 a

Hepatocellular carcinoma (HCC) remains a significant global health challenge with limited therapeutic options. Chemotherapy has major limitations, leading to a paradigm shift towards nanomedicines. In this study, we aimed to synthesize single and dual drug-loaded chitosan nanoparticles (CSNPs) containing 5-fluorouracil (5-FU) and ursolic acid (UA) for spatial and controlled delivery against HCC. We synthesized CSNPs by a modified bottom-up ionic gelation method and optimized various parameters to formulate particles with smaller sizes, uniform size distribution, and high surface charge for intra-arterial infusion for HCC. The characterization techniques confirmed a monodisperse population of smaller sized particles with average sizes, as determined from transmission electron microscopy (TEM), as 45.25 ± 11.58, 105.66 ± 10.96, 176 ± 16.46, and 220 ± 21.37 nm for CSNPs, 5-FU-CSNPs, UA-CSNPs and 5FU + UA-CSNPs respectively. These formulations exhibited excellent encapsulation of the drugs, with an initial pH-dependent rapid release of 5-FU followed by a subsequent slower and sustained release, while showing pH-dependent slow and controlled release of UA. The in vitro cell viability assay established the highest anticancer potential for 5FU + UA-CSNPs, followed by 5-FU-CSNPs and UA-CSNPs, and the lowest for plain drugs in the Hep3B cell line. An increased uptake of both the drugs in CSNPs substantiated the supremacy of these formulations over the plain drugs as drug delivery agents. These findings suggest that 5FU + UA-CSNPs, a novel formulation, works as a potent therapeutic agent against HCC, supporting our hypothesis of co-loading 5-FU and UA in CSNPs can effectively manage HCC, with particle attributes fit for administration via intra-arterial infusion.

1 aJaiswal, Neha1 aPawar, Anil, T.1 aChaudhari, Bhushan, P. uhttps://library.ncl.res.in/content/chitosan-nanoparticles-single-and-combinatorial-delivery-5-fluorouracil-and-ursolic-acid02211nas a2200157 4500008004100000245013900041210006900180260000800249300001400257490000600271520157800277100001901855700002301874700002701897856012901924 2025 eng d00aFunctionalized gold nanorods (GNRs) as a label for the detection of thyroid-stimulating hormone (TSH) through lateral flow assay (LFA)0 aFunctionalized gold nanorods GNRs as a label for the detection o cJAN a601–6170 v83 a

Thyroid-stimulating hormone (TSH) is an important biomarker of thyroid dysfunctions. Different types of nano biosensors have been developed to detect TSH. However, there is a lacuna in the development of sensitive paper-based nano biosensors due to less sensitivity and instability of the labels. Interestingly, the intense color and localized surface plasmon resonance (LSPR) property make gold nanorods (GNRs) an efficient lateral flow assay (LFA) label instead of spherical gold nanoparticles. The rapidity, simplicity and user-friendly functioning of LFAs make them a predominant technique compared to electrochemical techniques for point-of-care diagnosis. This proof-of-concept study underlines the role of stable GNRs as a novel label for LFAs. The TSH antibody functionalized GNRs can semi-quantitatively diagnose hyperthyroidism and hypothyroidism. The limit of detection (LOD) of the LFA is 0.3 µIU/mL, determined by visually analyzing the color development at the test line without employing any quantification method. Moreover, the study provides a comprehensive understanding of seed-mediated synthesis and functionalization of GNRs, which would benefit the researchers in suitably designing the GNRs-based labels for LFAs. Considering their exceptional characteristics, GNR-based LFAs are expected to surpass other labelling nanomaterials in detecting various biological markers. This enhanced sensitivity could significantly improve the clinical utility of diagnostic kits.

1 aVarma, Sanjana1 aBurade, Dimpal, K.1 aChaudhari, Bhushan, P. uhttps://library.ncl.res.in/content/functionalized-gold-nanorods-gnrs-label-detection-thyroid-stimulating-hormone-tsh-through02994nas a2200301 4500008004100000022001400041245017000055210006900225260000800294300001100302490000800313520193900321653001802260653001602278653001502294653001602309653001802325653002402343100002302367700001902390700002502409700002202434700003002456700002102486700002702507700002702534856013102561 2025 eng d a0882-401000aAn integrated paradigm to understand the antibacterial and antifungal potential of bimetallic core-shell platinum silver (Pt@Ag) nanoparticles: A one health approach0 aintegrated paradigm to understand the antibacterial and antifung cDEC a1081200 v2093 a

The concurrent occurrence of various microbial infections escalates the need to develop new treatments that can tackle multiple microbes and improve clinical outcomes. This study reports the synthesis and comprehensive evaluation of core-shell platinum-silver nanoparticles (Pt@AgNPs) designed to elucidate the antimicrobial effects while ensuring biocompatibility. The synthesis protocol was meticulously optimized to investigate the impact of precursor concentrations and reagent conditions. High-end characterization confirmed the formation of a welldefined core-shell structure with spherical morphology, crystalline nature, a face-centred cubic (FCC) lattice, high monodispersity, and stability, with a mean size of 20.344 +/- 4.492 nm. The antimicrobial potential of Pt@AgNPs was validated through a minimum inhibitory concentration (MIC) assay, revealing potent activity with MIC values of 15.6 mu g/mL for Pseudomonas aeruginosa and Staphylococcus aureus and 3.9 mu g/mL for Escherichia coli. Antibiofilm assay demonstrated significant inhibition of biofilm formation by P. aeruginosa at concentrations as low as 3.9 mu g/ml. The nanoparticles also exhibited notable antifungal activity, as indicated by an inhibition of 65.19 % for Aspergillus niger and 61.82 % for Fusarium verticillioides. Furthermore, hemocompatibility was noticed with the hemolysis assay, and the antioxidant properties of nanoparticles, assessed through the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, underscored their potential to mitigate oxidative stress. This integrative study positions Pt@AgNPs as a promising platform for combating the occurrence of co-infections. The core-shell nanoparticle serves as a versatile tool in antimicrobial defence, exhibiting antibacterial, antifungal, antibiofilm, and antioxidant activity. Thus, it highlights their commercial translational potential as a next-generation antimicrobial intervention.

10aAntibacterial10aantibiofilm10aantifungal10aAntioxidant10aCo-infections10aPt@Ag nanoparticles1 aBamb, Aagam, Lalit1 aVarma, Sanjana1 aGade, Tejas, Subhash1 aPalaskar, Shahaji1 aVamkudoth, Koteswara, Rao1 aVyawahare, Niraj1 aChaudhari, Pallavi, M.1 aChaudhari, Bhushan, P. uhttps://library.ncl.res.in/content/integrated-paradigm-understand-antibacterial-and-antifungal-potential-bimetallic-core-shell03051nas a2200217 4500008004100000245013700041210006900178260000800247300000900255490000700264520225300271100001902524700002302543700002202566700002302588700001902611700001802630700003002648700002702678856012802705 2025 eng d00aUnderstanding the antimicrobial and antioxidant potential of bioinspired platinum nanoparticles synthesized using ocimum tenuiflorum0 aUnderstanding the antimicrobial and antioxidant potential of bio cJAN a17890 v153 a

The emergence of infectious diseases has highlighted the need for novel and effective antimicrobial agents. Platinum nanoparticles (PtNPs) could act as a potent antimicrobial agent due to their unique physicochemical properties. However, the chemical or physical synthesis methods of PtNPs have drawbacks, like irregular shape and use of toxic chemicals. These drawbacks can be effectively overcome by using the biological method synthesis. Thus, this study presents the green synthesis of platinum nanoparticles (PtNPs) using Ocimum tenuiflorum leaf extract as a bio-reductant. The study also comprehensively encompasses the role of multiple process parameters for the green synthesis of PtNPs. The high-resolution transmission electron microscopy (HR-TEM) revealed spherical PtNPs with an average size of 2.36 ± 0.5 nm that exhibits excellent stability (zeta potential, −45.67 ± 2.57 mV). Phytochemical analysis of aqueous O. tenuiflorum leaf extract was performed to assess the bioactive compounds like saponins, phenols, and tannins, reducing sugars and flavonoids. The PtNPs demonstrated significant antioxidant activity of 86.9 ± 0.12% at 10 µg/mL. Further, the biosynthesized PtNPs showed efficient antimicrobial activity with a minimum inhibitory concentration (MIC) of 6.25 µg/mL against Escherichia coli and 1.5 µg/mL against Staphylococcus aureus. These findings highlight the therapeutic potential of eco-friendly PtNPs for pharmaceutical and biotechnological applications.

1 aVarma, Sanjana1 aBamb, Aagam, Lalit1 aTambe, Sayali, A.1 aBurade, Dimpal, K.1 aJagdale, Swati1 aPande, Bharat1 aVamkudoth, Koteswara, Rao1 aChaudhari, Bhushan, P. uhttps://library.ncl.res.in/content/understanding-antimicrobial-and-antioxidant-potential-bioinspired-platinum-nanoparticles