02008nas a2200169   4500008004100000245009300041210006900134260000800203300001400211490000800225520140700233100002001640700001801660700002101678700001701699856012201716       2016                        eng d00aInhibition of quorum-sensing-mediated biofilm formation in Cronobacter sakazakii strains0 aInhibition of quorumsensingmediated biofilm formation in Cronoba  cSEP  a1708-17140 v1623 aThe present study investigated plant extracts for their anti-quorum-sensing (QS) potential to inhibit the biofilm formation in Cronobacter sakazakii strains. The bioassay based on loss of pigment production by Chromobacterium violaceum 026 and Agrobacterium tumefaciens NTL4 (pZLR4) was used for initial screening of the extracts. Further, the effect of extracts on the inhibition of QS-mediated biofilm in C. sakazakii isolates was evaluated using standard crystal violet assay. The effect on biofilm texture was studied using SYTO9 staining and light and scanning electron microscopy. Among the tested extracts, Piper nigrum and Cinnamomum verum at 100 ppm resulted in 78 and 68% reduction in the production of violacein as well as blue-green colour in both biosensor strains. A higher inhibitory activity (>50 %) on biofilm formation in C. sakazakii was observed for Pip. nigrum and Cin. verum, whereas the other extracts possessed moderate (25-50 %) and minimal (<25 %) inhibitory activities. Further, the fluorescent and scanning electron microscopic images indicated a major disruption in the architecture of biofilms of tested strains by Pip. nigrum. This study points to the possibility of using Pip. nigrum and Cin. verum as inhibitor of QS-mediated biofilm formation by C. sakazakii that could be further explored for novel bioactive molecules to limit the emerging infections of C. sakazakii1 aSingh, Niharika1 aPatil, Amrita1 aPrabhune, Asmita1 aGoel, Gunjan  uhttps://library.ncl.res.in/content/inhibition-quorum-sensing-mediated-biofilm-formation-cronobacter-sakazakii-strains01469nas a2200181   4500008004100000245010400041210006900145260000800214300001200222490000600234520081300240100002001053700001801073700003101091700001801122700001701140856013001157       2017                        eng d00aDiverse profiles of N-acyl-homoserine lactones in biofilm forming isolates of Cronobacter sakazakii0 aDiverse profiles of Nacylhomoserine lactones in biofilm forming   cOCT  a275-2810 v83 a<p>The present study investigates the role of quorum sensing (QS) molecules expressed by C. sakazakii in biofilm formation and extracellular polysaccharide expression. The QS signaling was detected using Chromobacterium violaceum 026 and Agrobacterium tumefaciens NTL4(pZLR4) based bioassay. Long chain N-acyl-homoserine lactones (AHLs) with C6- C18 chain length were identified using High Performance Liquid Chromatography and Liquid Chromatography-High Resolution Mass Spectrometry. A higher Specific Biofilm Formation (SBF) index (p &lt; 0.05) with the presence of genes associated with cellulose biosynthesis (bcsA, bcsC and bcsG) was observed in the strains. AHLs and their mechanisms can serve as novel targets for developing technologies to eradicate and prevent biofilm formation by C. sakazakii.</p>1 aSingh, Niharika1 aPatil, Amrita1 aPrabhune, Asmita, Ashutosh1 aRaghav, Mamta1 aGoel, Gunjan  uhttps://library.ncl.res.in/content/diverse-profiles-n-acyl-homoserine-lactones-biofilm-forming-isolates-cronobacter-sakazakii