01811nas a2200229   4500008004100000022001400041245009700055210006900152260006700221300001400288490000700302520091800309653003001227653003201257653003001289653004001319653004301359100002001402700001601422700001901438856012401457       2012                        eng d  a8756-793800aIn situ pH maintenance for mammalian cell cultures in shake flasks and tissue culture flasks0 aIn situ pH maintenance for mammalian cell cultures in shake flas  a111 RIVER ST, HOBOKEN 07030-5774, NJ USAbWILEY-BLACKWELLcNOV  a1605-16100 v283 a<p>pH in animal cell cultures decreases due to production of metabolites like lactate. pH control via measurement and base addition is not easily possible in small-scale culture formats like tissue-culture flasks and shake flasks. A hydrogel-based system is reported for in situ pH maintenance without pH measurement in such formats, and is demonstrated to maintain pH between 6.8 and 7.2 for a suspension CHO cell line in CD CHO medium and between 7.3 and 7.5 for adherent A549 cells in DMEM:F12 containing 10% FBS. This system for pH maintenance, along with our previous report of hydrogels for controlled nutrient delivery in shake flasks can allow shake flasks to better mimic bioreactor-based fed batch operation for initial screening during cell line and process development for recombinant protein production in mammalian cells. (C) 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012</p>10ahigh throughput screening10apH control for cell culture10apH control in shake flask10apH control in tissue culture flasks10asmall scale platforms for cell culture1 aPradhan, Ketaki1 aPant, Tejal1 aGadgil, Mugdha  uhttps://library.ncl.res.in/content/situ-ph-maintenance-mammalian-cell-cultures-shake-flasks-and-tissue-culture-flasks-002344nas a2200229   4500008004100000022001400041245016900055210006900224260006700293300001200360490000700372520139200379653002001771653002001791653002101811653003001832653002401862653003901886653003901925100001901964856013101983       2015                        eng d  a0268-257500aDevelopment of a mathematical model for animal cell culture without pH control and its application for evaluation of clone screening outcomes in shake flask culture0 aDevelopment of a mathematical model for animal cell culture with  a111 RIVER ST, HOBOKEN 07030-5774, NJ USAbWILEY-BLACKWELLcJAN  a166-1750 v903 a<p>BACKGROUNDCurrently used mathematical models for animal cell growth and metabolism cannot simulate changing culture pH and its effect on metabolism, and hence cannot accurately model cultures lacking pH control. Animal cell cultures are however widely cultured at a small scale without pH control, e.g. for clone screening during cell line development. A mathematical model for animal cell growth without pH control is developed and applied to design optimal conditions for clone screening without pH control. RESULTSThe mathematical model successfully simulates published data for suspension CHO culture in shake flasks. It is further used to simulate clonal heterogeneity and predict relative clone performance in different culture conditions. Predictions of correlation coefficients between clone titers in batch and fed batch screening conditions and pH controlled fed batch agree with published values. Fed batch mode is predicted to give better screening outcomes than batch, but only for clones able to consume lactate. Simulations reveal that culture duration of the screening assay has a large effect on the screening outcome and optimal durations differ based on the ability or inability of clones to consume lactate. CONCLUSIONThis study presents a tool for optimal design of screening assay for clone selection without pH control. (c) 2014 Society of Chemical Industry</p>10aclone screening10aclone selection10aculture duration10ahigh throughput screening10alactate consumption10amathematical model with pH effects10apH control for animal cell culture1 aGadgil, Mugdha  uhttps://library.ncl.res.in/content/development-mathematical-model-animal-cell-culture-without-ph-control-and-its-application-0