02644nas a2200289 4500008004100000022001400041245008600055210006900141260000800210300001400218490000700232520174800239653001901987653001302006653001702019653001702036100001802053700001802071700002802089700002102117700002202138700002102160700002302181700002102204700002402225856010502249 2018 eng d a1466-502600aMicrobacterium telephonicum sp nov., isolated from the screen of a cellular phone0 aMicrobacterium telephonicum sp nov isolated from the screen of a cAPR a1052-10580 v683 a

A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63(T), exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a member of Microbacterium, and most closely related to Microbacterium aurantiacum IFO 15234(T) and Microbacterium kitamiense Kitami C2(T). The DNA-DNA relatedness values of the strain S2T63(T) to M. aurantiacum KACC 20510(T), M. kitamiense KACC 20514(T) and Microbacterium laevaniformans KACC 14463(T) were 65% (+/- 4), 29.5% (+/- 3) and 55.9% (+/- 4), respectively. The genomic DNA G+C content was 71.8 mol%. The major fatty acids were anteiso C-15:0, iso-C-16 : 0, C-16 : 0 and anteiso-C-17 : 0. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified polar lipids. The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1 alpha. The predominant menaquinones were MK-12 and MK-13. Rhamnose, fucose and galactose were the whole-cell sugars detected. The strain also showed biofilm production, estimated by using crystal violet assay. Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacterium telephonicum is proposed, with S2T63(T) (=MCC 2967 T = KACC 18715 T = LMG 29293(T)) as the type strain.

10aactinobacteria10abio-film10aMALDI-TOF MS10amobile phone1 aRahi, Praveen1 aKurli, Rashmi1 aPansare, Aabeejjeet, N.1 aKhairnar, Mitesh1 aJagtap, Shubhangi1 aPatel, Nisha, B.1 aDastager, Syed, G.1 aLawson, Paul, A.1 aShouche, Yogesh, S. uhttps://library.ncl.res.in/content/microbacterium-telephonicum-sp-nov-isolated-screen-cellular-phone03154nas a2200289 4500008004100000022001400041245016500055210006900220260000800289300001200297490000800309520217000317653002002487653001102507653001702518653002402535653001102559100001902570700001602589700002302605700002102628700001802649700002302667700002302690700002202713856012902735 2019 eng d a0048-969700aMetagenomic insights to understand transient influence of Yamuna River on taxonomic and functional aspects of bacterial and archaeal communities of River Ganges0 aMetagenomic insights to understand transient influence of Yamuna cJUL a288-2990 v6743 a

River confluences are interesting ecosystems to investigate for their microbial community structure and functional potentials. River Ganges is one of the most important and holy river of India with great mythological history and religious significance. The Yamuna River meets Ganges at the Prayagraj (formerly known as Allahabad), India to form a unique confluence. The influence of Yamuna River on taxonomic and functional aspects of microbiome at this confluence and its downstream, remains unexplored. To unveil this dearth, whole metagenome sequencing of the microbial (bacterial and archaeal) community from the sediment samples of December 2017 sampling expedition was executed using high throughput MinION technology. Results revealed differences in the relative abundance of bacterial and archaeal communities across the confluence. Grouped by the confluence, a higher abundance of Proteobacteria and lower abundance of Bacteroidetes and Firnacutes was observed for Yamuna River (G15Y) and at immediate downstream of confluence of Ganges (G15DS), as compared to the upstream, confluence, and farther downstream of confluence. A similar trend was observed for archaeal communities with a higher abundance of Euryarchaeoto in G15Y and G15DS, indicating Yamuna River's influence. Functional gene(s) analysis revealed the influence of Yamuna River on xenobiotic degradation, resistance to toxic compounds, and antibiotic resistance interceded by the autochthonous microbes at the confluence and succeeding downstream locations. Overall, similar taxonomic and functional profiles of microbial communities before confluence (upstream of Ganges) and farther downstream of confluence, suggested a transient influence of Yamuna River. Our study is significant since it may be foundational basis to understand impact of Yamuna River and also rare event of mass bathing on the microbiome of River Ganges. Further investigation would be required to understand, the underlying cause behind the restoration of microbial profiles post-confluence farther zone, to unravel the rejuvenation aspects of this unique ecosystem. (C) 2019 Elsevier B.A. All rights reserved.

10aConfluence zone10aGanges10aMetagenomics10aTransient influence10aYamuna1 aSamson, Rachel1 aShah, Manan1 aYadav, Rakeshkumar1 aSarode, Priyanka1 aRajput, Vinay1 aDastager, Syed, G.1 aDharne, Mahesh, S.1 aKhairnar, Krishna uhttps://library.ncl.res.in/content/metagenomic-insights-understand-transient-influence-yamuna-river-taxonomic-and-functional00722nas a2200205 4500008004100000022001400041245009200055210006900147260000800216300000900224490000700233100002500240700001700265700002400282700002400306700001800330700003000348700002300378856011500401 2019 eng d a1466-502600aMicrobacterium enclense sp. nov., isolated from sediment sample (vol 65, pg 2064, 2015)0 aMicrobacterium enclense sp nov isolated from sediment sample vol cMAY a15280 v691 aMawlankar, Rahul, R.1 aMual, Poonam1 aSonalkar, Vidya, V.1 aThorat, Meghana, N.1 aVerma, Ashish1 aSrinivasan, Krishnamurthi1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/microbacterium-enclense-sp-nov-isolated-sediment-sample-vol-65-pg-2064-201502408nas a2200253 4500008004100000022001400041245014400055210006900199260000800268300001200276490000700288520152200295100002201817700001901839700002001858700001901878700001801897700001601915700002601931700002501957700002301982700002502005856012402030 2020 eng d a1554-892900aMolecular networking and whole-genome analysis aid discovery of an angucycline that inactivates mTORC1/C2 and induces programmed cell death0 aMolecular networking and wholegenome analysis aid discovery of a cMAR a780-7880 v153 a

Rediscovery of known compounds and time consumed in identification, especially high molecular weight compounds with complex structure, have let down interest in drug discovery. In this study, whole-genome analysis of microbe and Global Natural Products Social (GNPS) molecular networking helped in initial understanding of possible compounds produced by the microbe. Genome data revealed 10 biosythethic gene clusters that encode for secondary metabolites with anticancer potential. NMR analysis of the pure compound revealed the presence of a four-ringed benz[a]anthracene, thus confirming angucycline; molecular networking further confirmed production of this class of compounds. The type II polyketide synthase gene identified in the microbial genome was matched with the urdamycin duster by BLAST analysis. This information led to ease in identification of urdamycin E and a novel natural derivative, urdamycin V, purified from Streptomyces sp. OA293. Urdamycin E (Urd E) induced apoptosis and autophagy in cancer cell lines. Urd E exerted anticancer action through inactivation of the mTOR complex by preventing phosphorylation at Ser 2448 and Ser 2481 of mTORC1 and mTORC2, respectively. Significant reduction in phosphorylation of the major downstream regulators of both mTORC1 (p70s6k and 4e-bp1) and mTORC2 (Akt) were observed, thus further confirming complete inhibition of the mTOR pathway. Urd E presents itself as a novel mTOR inhibitor that employs a novel mechanism in mTOR pathway inhibition.

1 aDan, Vipin, Mohan1 aVinodh, J., S.1 aSandesh, C., J.1 aSanawar, Rahul1 aLekshmi, Asha1 aKumar, Ajay1 aKumar, T., R. Santhos1 aMarelli, Uday, Kiran1 aDastager, Syed, G.1 aPillai, Radhakrishna uhttps://library.ncl.res.in/content/molecular-networking-and-whole-genome-analysis-aid-discovery-angucycline-inactivates02221nas a2200253 4500008004100000022001400041245015000055210006900205260000800274300001100282490000800293520134100301653001901642653002201661653001501683653001401698653001401712653002301726100002001749700002001769700002301789700002301812856013201835 2021 eng d a0031-942200aMolecular insights of fungal endophyte co-inoculation with Trichoderma viride for the augmentation of forskolin biosynthesis in Coleus forskohlii0 aMolecular insights of fungal endophyte coinoculation with Tricho cAPR a1126540 v1843 a

To understand the compatibility of three native endophytic fungi Phialemoniopsis cornearis (SF1), Macrophomina pseudophaseolina (SF2) and Fusarium redolens (RF1) with Trichoderma viride (TV1) on Coleus forskohlii in enhancing plant growth and forskolin content, field experiments were conducted. Co-inoculation of RF1+TV1 showed significant improvement in plant growth (52%), root biomass (67%), and in planter forskolin content (94%), followed by treatment with SF2+TV1 and SF1+TV1. qRT-PCR was carried out to quantify expression of five key forskolin biosynthetic pathway genes (CfTPS2, CfTPS3, CfTPS4, CfCYP76AH15, and CfACT1-8) in RF1+TV1 treated C. forskohlii plants. Elevated expression of CfTPS2, CfTPS4, CfCYP76AH15 and CfACT1-8 genes was observed with RF1+TV1 combination as compared to uninoculated C. forskohlii plants. Besides, RF1+TV1 treatment considerably reduced the severity of nematode infection of C. forskohlii plants under field conditions. Thus, congruent properties of F. redolens (RF1) were witnessed with co-inoculation of T. viride (TV1) under field conditions which resulted in enhanced forskolin content, root biomass, and reduced nematode infections in C. forskohlii. Overall, this approach could be an economical and sustainable step towards cultivation of commercially important medicinal plants.

10aCo-inoculation10aColeus forskohlii10aendophytes10aForskolin10aLamiaceae10aTrichoderma viride1 aMastan, Anthati1 aRane, Digeshwar1 aDastager, Syed, G.1 aBabu, C., S. Vivek uhttps://library.ncl.res.in/content/molecular-insights-fungal-endophyte-co-inoculation-trichoderma-viride-augmentation-forskolin04223nas a2200205 4500008004100000245009500041210006900136260000800205520345300213100002203666700002503688700002603713700002503739700002803764700002203792700002303814700002903837700002903866856012203895 2024 eng d00aMultifunctional metabolites of Streptomyces kunmingensis BS19 from bamboo rhizosphere soil0 aMultifunctional metabolites of Streptomyces kunmingensis BS19 fr cNOV3 a

A pigmented bioactive molecule from Streptomyces kunmingensis BS19 was isolated, characterized, and evaluated for anti-infective, antiproliferative, and wound-healing properties. The yellow–red pigment produced from the strain BS19 showed promising activity against methicillin-resistant Staphylococcus aureus (MRSA) and Mycobacterium tuberculosis. Extracellular bioactive pigment from the strain BS19 was produced by agar surface fermentation and purified through bioassay guided preparative HPLC-based purification. Based on the results of UV, FT-IR, GC–MS, 1H NMR, and 13C NMR spectral analyses, the purified pigment was identified as a chromopeptide class of molecule with phenoxazinone chromophore. Its molecular weight was determined as the chemical formula C64H90N12O16 and molecular weight 1283 g/mol. It exhibited promising antimicrobial activity against Staphylococcus aureus (ATCC 1720) and antiproliferative activity against 14 types of human cancer cell lines. It showed good in vivo wound-healing activity in the rat model. The present study explored Streptomyces kunmingensis as a newly added source for the isolation of chromopeptide antibiotics for antimicrobial, wound-healing, and anticancer applications.

1 aSangeetha, Murthy1 aSasirekha, Sivakumar1 aMahendran, Jaganathan1 aSivarajan, Anbalmani1 aRadhakrishnan, Manikkam1 aManigundan, Kaari1 aDastager, Syed, G.1 aSengottuvelu, Singaravel1 aBalagurunathan, Ramasamy uhttps://library.ncl.res.in/content/multifunctional-metabolites-streptomyces-kunmingensis-bs19-bamboo-rhizosphere-soil