02413nas a2200289 4500008004100000022001400041245010300055210006900158260000800227300001100235490000800246520145800254653001401712653002701726653002401753653001501777653001101792100002401803700002301827700002901850700002501879700002201904700002301926700001901949700002801968856012701996 2020 eng d a0882-401000aAntimicrobial profiling of coral reef and sponge associated bacteria from southeast coast of India0 aAntimicrobial profiling of coral reef and sponge associated bact cAPR a1039720 v1413 a

Culturable bacteria associated with marine sponges and coral mucus (collected from Gulf of Mannar and Palk Bay) were screened for their prospective antimicrobial compounds against 9 bacterial pathogens (Bacillus megaterium, B. cereus, Salmonella typhimurium, Staphylococcus aureus, Proteus vulgaris, Klebsillla pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii) and a fungal pathogen (Candida albicans). Of the 263 bacterial isolates obtained during this study, 52 isolates displayed antimicrobial activity against one or more pathogens. 16S rRNA gene sequencing revealed that these 52 strains affiliated to 14 genera from three phyla Proteobacteria, Firmicutes and Actinobacteria. Sponge associated bacterial strains F-04, 1-23, 1-33 and G-03 inhibited the growth of all the bacterial pathogens tested in this study and significantly the former 2 strains inhibited the growth of fungal pathogen also. Majority of the potential strains (88.4% out of 52 strains) inhibited the growth of Bacillus cereus. Interestingly, an actinomycete strain F-04 (isolated from sponge Orina sagittaria) inhibited the growth of methicillin resistant Staphylococcus aureus. In total, 10 volatile organic compounds were determined from the ethyl acetate and hexane extract of the strain F-04 using GC-MS. Overall, marine bacteria isolated during this study demonstrate the potential for the development of broad spectrum antibiotics.

10aAnti-MRSA10aAntimicrobial activity10aBacterial diversity10aCoral reef10aSponge1 aRajasabapathy, Raju1 aGhadi, Sanjeev, C.1 aManikandan, Balakrishnan1 aMohandass, Chellandi1 aSurendran, Akhila1 aDastager, Syed, G.1 aMeena, Ram, M.1 aJames, Rathinam, Arthur uhttps://library.ncl.res.in/content/antimicrobial-profiling-coral-reef-and-sponge-associated-bacteria-southeast-coast-india02270nas a2200241 4500008004100000245007000041210006900111260000900180300001600189490000700205520148900212100002201701700002301723700002401746700001601770700002001786700002801806700002501834700002301859700002101882700002301903856010201926 2020 eng d00aApproach to nigericin derivatives and their therapeutic potential0 aApproach to nigericin derivatives and their therapeutic potentia cNOV  a43085-430910 v103 a

A new nigericin analogue that has been chemically modified was synthesized through a fluorination process from the parent nigericin, produced from a novel Streptomyces strain DASNCL-29. Fermentation strategies were designed for the optimised production of nigericin molecule and subjected for purification and structural analysis. The fermentation process resulted in the highest yield of nigericin (33% (w/w)). Initially, nigericin produced from the strain DASNCL-29 demonstrated polymorphism in its crystal structure, i.e., monoclinic and orthorhombic crystal lattices when crystallised with methanol and hexane, respectively. Furthermore, nigericin produced has been subjected to chemical modification by fluorination to enhance its efficacy. Two fluorinated analogues revealed that they possess a very potent antibacterial activity against Gram positive and Gram negative bacteria. To date, the nigericin molecule has not been reported for any reaction against Gram-negative bacteria, which are increasingly becoming resistant to antibiotics. For the first time, fluorinated analogues of nigericin have shown promising activity. In vitro cytotoxicity analysis of fluorinated analogues demonstrated tenfold lesser toxicity than the parent nigericin. This is the first type of study where the fluorinated analogues of nigericin showed very encouraging activity against Gram-negative organisms; moreover, they can be used as a candidate for treating many serious infections.

1 aSahu, Amit, Kumar1 aSaid, Madhukar, S.1 aHingamire, Tejashri1 aGaur, Megha1 aKhan, Abujunaid1 aShanmugam, Dhanasekaran1 aBarvkar, Vitthal, T.1 aDharne, Mahesh, S.1 aBharde, Atul, A.1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/approach-nigericin-derivatives-and-their-therapeutic-potential01730nas a2200241 4500008004100000022001400041245011000055210006900165260000800234300001200242490000700254520094300261653000601204653000601210653002501216653001601241653001101257653002401268100002301292700002301315700002301338856012701361 2022 eng d a1139-670900aAntifungal compound from marine Serratia marcescens BKACT and its potential activity against Fusarium sp.0 aAntifungal compound from marine Serratia marcescens BKACT and it cNOV a851-8620 v253 a

Ecofriendly biocontrol agents to control pathogenic fungi are in demand globally. The present study evaluated the antifungal potentials of marine bacteria Serratia marcescens BKACT against eight different Fusarium species. A highest 75.5 +/- 0.80% of mycelial inhibition was observed against Fusarium foetens NCIM 1330. Structural characterization of the purified compound was analyzed by GC-MS and NMR techniques; based on the analysis, it is confirmed as 2, 4-di-tert butyl phenol (2, 4-DTBP) with chemical structure C14H22O. At 0.53 mM concentration, purified compound inhibited complete spore germination of F. foetens NCIM 1330. In vitro assay showed complete inhibition of F. foetens NCIM 1330 on the wheat seeds. Tested concentration does not show any toxic effect on germination of the seeds. By this study, we conclude that, 2, 4-DTBP is a suitable candidate to be used as biocontrol agent against Fusarium infection.

10a210a410adi-tert butyl phenol10aFusarium sp10amarine10aSerratia marcescens1 aKharat, Bharat, A.1 aSaid, Madhukar, S.1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/antifungal-compound-marine-serratia-marcescens-bkact-and-its-potential-activity-against01648nas a2200205 4500008004100000022001400041245009900055210006900154260000800223300001400231490000700245520091000252100002901162700003001191700002201221700002301243700002401266700002201290856013001312 2022 eng d a0265-056800aArtificial intelligence in microbial natural product drug discovery: current and emerging role0 aArtificial intelligence in microbial natural product drug discov cDEC a2215-22300 v393 a

Covering: up to the end of 2022 Microorganisms are exceptional sources of a wide array of unique natural products and play a significant role in drug discovery. During the golden era, several life-saving antibiotics and anticancer agents were isolated from microbes; moreover, they are still widely used. However, difficulties in the isolation methods and repeated discoveries of the same molecules have caused a setback in the past. Artificial intelligence (AI) has had a profound impact on various research fields, and its application allows the effective performance of data analyses and predictions. With the advances in omics, it is possible to obtain a wealth of information for the identification, isolation, and target prediction of secondary metabolites. In this review, we discuss drug discovery based on natural products from microorganisms with the help of AI and machine learning.

1 aSahayaSheela, Vinodh, J.1 aLankadasari, Manendra, B.1 aDan, Vipin, Mohan1 aDastager, Syed, G.1 aPandian, Ganesh, N.1 aSugiyama, Hiroshi uhttps://library.ncl.res.in/content/artificial-intelligence-microbial-natural-product-drug-discovery-current-and-emerging-role02733nas a2200349 4500008004100000022001400041245012900055210006900184260000800253300001400261490000800275520154800283653001901831653002001850653001301870653001801883653002601901100002801927700002201955700002801977700002202005700002202027700001902049700002702068700002202095700002902117700003802146700002302184700002702207700001802234856013102252 2023 eng d a0273-228900aAntibacterial and anti-HIV metabolites from marine streptomyces albus MAB56 isolated from Andaman and Nicobar Islands, India0 aAntibacterial and antiHIV metabolites from marine streptomyces a cDEC a7738-77540 v1953 a

Marine-derived actinobacteria have tremendous potential to produce novel metabolites with diverse biological activities. The Andaman coast of India has a lot of microbial diversity, but it is still a relatively unknown ecology for isolating novel actinobacteria with beneficial bioactive compounds. We have isolated 568 actinobacterial strains from mangrove rhizosphere sediments and sponge samples. Crude extracts from 75 distinct strains were produced by agar surface fermentation and extracted using ethyl acetate. In the disc diffusion method, 25 actinobacterial strains showed antimicrobial activity; notably, the strain MAB56 demonstrated promising broad-spectrum activity. Strain MAB56 was identified as Streptomyces albus by cultural, microscopic, and molecular methods. Conditions for bioactive metabolites from MAB56 were optimized and produced in a lab-scale fermenter. Three active metabolites (C1, C2, and C3) that showed promising broad-spectrum antimicrobial activity were isolated through HPLC-based purification. Based on the UV, FT-IR, NMR, and LC-MS analysis, the chemical nature of the active compounds was confirmed as 12-methyltetradecanoic acid (C1), palmitic acid (C2), and tridecanoic acid (C3) with molecular formulae C14H28O2, C16H32O2, and C13H26O2, respectively. Interestingly, palmitic acid (C2) also exhibited anti-HIV activity with an IC50 value of < 1 mu g/ml. Our findings reveal that the actinobacteria from the Andaman marine ecosystems are promising for isolating anti-infective metabolites.

10aactinobacteria10aAndaman Islands10aanti-HIV10aAntibacterial10aBioactive metabolites1 aManikkam, Radhakrishnan1 aMurthy, Sangeetha1 aPalaniappan, Sivasankar1 aKaari, Manigundan1 aSahu, Amit, Kumar1 aSaid, Madhukar1 aGanesan, Vijayalakshmi1 aKannan, Sivakumar1 aRamasamy, Balagurunathan1 aThirugnanasambandan, Somasundaram1 aDastager, Syed, G.1 aHanna, Luke, Elizabeth1 aKumar, Vanaja uhttps://library.ncl.res.in/content/antibacterial-and-anti-hiv-metabolites-marine-streptomyces-albus-mab56-isolated-andaman-and