02644nas a2200289   4500008004100000022001400041245008600055210006900141260000800210300001400218490000700232520174800239653001901987653001302006653001702019653001702036100001802053700001802071700002802089700002102117700002202138700002102160700002302181700002102204700002402225856010502249       2018                        eng d  a1466-502600aMicrobacterium telephonicum sp nov., isolated from the screen of a cellular phone0 aMicrobacterium telephonicum sp nov isolated from the screen of a  cAPR  a1052-10580 v683 a<p>A cultivation-based study of the microbial diversity of cellular phone screens led to the isolation of a Gram-stain-positive, aerobic, rod-shaped and non-endospore-forming bacterium, designated S2T63(T), exhibiting phenotypic and genotypic characteristics unique to the type strains of closely related species. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a member of Microbacterium, and most closely related to Microbacterium aurantiacum IFO 15234(T) and Microbacterium kitamiense Kitami C2(T). The DNA-DNA relatedness values of the strain S2T63(T) to M. aurantiacum KACC 20510(T), M. kitamiense KACC 20514(T) and Microbacterium laevaniformans KACC 14463(T) were 65% (+/- 4), 29.5% (+/- 3) and 55.9% (+/- 4), respectively. The genomic DNA G+C content was 71.8 mol%. The major fatty acids were anteiso C-15:0, iso-C-16 : 0, C-16 : 0 and anteiso-C-17 : 0. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified polar lipids. The peptidoglycan contained the amino acids glycine, lysine, alanine and glutamic acid, with substantial amounts of hydroxy glutamic acid detected, which is characteristic of peptidoglycan type B1 alpha. The predominant menaquinones were MK-12 and MK-13. Rhamnose, fucose and galactose were the whole-cell sugars detected. The strain also showed biofilm production, estimated by using crystal violet assay. Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new strain represents a novel species of the genus Microbacterium, for which the name Microbacterium telephonicum is proposed, with S2T63(T) (=MCC 2967 T = KACC 18715 T = LMG 29293(T)) as the type strain.</p>10aactinobacteria10abio-film10aMALDI-TOF MS10amobile phone1 aRahi, Praveen1 aKurli, Rashmi1 aPansare, Aabeejjeet, N.1 aKhairnar, Mitesh1 aJagtap, Shubhangi1 aPatel, Nisha, B.1 aDastager, Syed, G.1 aLawson, Paul, A.1 aShouche, Yogesh, S.  uhttps://library.ncl.res.in/content/microbacterium-telephonicum-sp-nov-isolated-screen-cellular-phone02733nas a2200349   4500008004100000022001400041245012900055210006900184260000800253300001400261490000800275520154800283653001901831653002001850653001301870653001801883653002601901100002801927700002201955700002801977700002202005700002202027700001902049700002702068700002202095700002902117700003802146700002302184700002702207700001802234856013102252       2023                        eng d  a0273-228900aAntibacterial and anti-HIV metabolites from marine streptomyces albus MAB56 isolated from Andaman and Nicobar Islands, India0 aAntibacterial and antiHIV metabolites from marine streptomyces a  cDEC  a7738-77540 v1953 a<p>
	Marine-derived actinobacteria have tremendous potential to produce novel metabolites with diverse biological activities. The Andaman coast of India has a lot of microbial diversity, but it is still a relatively unknown ecology for isolating novel actinobacteria with beneficial bioactive compounds. We have isolated 568 actinobacterial strains from mangrove rhizosphere sediments and sponge samples. Crude extracts from 75 distinct strains were produced by agar surface fermentation and extracted using ethyl acetate. In the disc diffusion method, 25 actinobacterial strains showed antimicrobial activity; notably, the strain MAB56 demonstrated promising broad-spectrum activity. Strain MAB56 was identified as Streptomyces albus by cultural, microscopic, and molecular methods. Conditions for bioactive metabolites from MAB56 were optimized and produced in a lab-scale fermenter. Three active metabolites (C1, C2, and C3) that showed promising broad-spectrum antimicrobial activity were isolated through HPLC-based purification. Based on the UV, FT-IR, NMR, and LC-MS analysis, the chemical nature of the active compounds was confirmed as 12-methyltetradecanoic acid (C1), palmitic acid (C2), and tridecanoic acid (C3) with molecular formulae C14H28O2, C16H32O2, and C13H26O2, respectively. Interestingly, palmitic acid (C2) also exhibited anti-HIV activity with an IC50 value of &lt; 1 mu g/ml. Our findings reveal that the actinobacteria from the Andaman marine ecosystems are promising for isolating anti-infective metabolites.</p>
10aactinobacteria10aAndaman Islands10aanti-HIV10aAntibacterial10aBioactive metabolites1 aManikkam, Radhakrishnan1 aMurthy, Sangeetha1 aPalaniappan, Sivasankar1 aKaari, Manigundan1 aSahu, Amit, Kumar1 aSaid, Madhukar1 aGanesan, Vijayalakshmi1 aKannan, Sivakumar1 aRamasamy, Balagurunathan1 aThirugnanasambandan, Somasundaram1 aDastager, Syed, G.1 aHanna, Luke, Elizabeth1 aKumar, Vanaja  uhttps://library.ncl.res.in/content/antibacterial-and-anti-hiv-metabolites-marine-streptomyces-albus-mab56-isolated-andaman-and