02223nas a2200289 4500008004100000245012600041210006900167260000800236300000900244490000700253520117200260653001301432653001301445653002001458653002101478653002601499653002601525100003801551700004201589700002301631700002801654700002701682700002701709700003701736700003401773856012601807 2020 eng d00aBacterial biofilm formation using PCL/curcumin electrospun fibers and its potential use for biotechnological applications0 aBacterial biofilm formation using PCLcurcumin electrospun fibers cDEC a55560 v133 a

Electrospun nanofibers are used for many applications due to their large surface area, mechanical properties, and bioactivity. Bacterial biofilms are the cause of numerous problems in biomedical devices and in the food industry. On the other hand, these bacterial biofilms can produce interesting metabolites. Hence, the objective of this study is to evaluate the efficiency of poly (x190;- caprolactone)/Curcumin (PCL/CUR) nanofibers to promote bacterial biofilm formation. These scaffolds were characterized by scanning electron microscopy (SEM), which showed homogeneous fibers with diameters between 441-557 nm; thermogravimetric analysis and differential scanning calorimetry (TGA and DSC) demonstrated high temperature resilience with degradation temperatures over >350 degrees C; FTIR and H-1-NMR serve as evidence of CUR incorporation in the PCL fibers. PCL/CUR scaffolds successfully promoted the formation of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa biofilms. These results will be valuable in the study of controlled harvesting of pathogenic biofilms as well as in metabolites production for biotechnological purposes.

10aBacteria10acurcumin10aelectrospinning10aEscherichia coli10aPseudomona aeruginosa10aStaphylococcus aureus1 aPompa-Monroy, Daniella, Alejandra1 aFigueroa-Marchant, Paulina, Guadalupe1 aDastager, Syed, G.1 aThorat, Meghana, Namdeo1 aIglesias, Ana, Leticia1 aMiranda-Soto, Valentin1 aPerez-Gonzalez, Graciela, Lizeth1 aVillarreal-Gomez, Luis, Jesus uhttps://library.ncl.res.in/content/bacterial-biofilm-formation-using-pclcurcumin-electrospun-fibers-and-its-potential-use03393nas a2200397 4500008004100000245009600041210006900137260000800206300000800214490000700222520206400229653002002293653003202313653002902345653002002374653002002394100003102414700002302445700002402468700001902492700002302511700003102534700003702565700002502602700002702627700002302654700003202677700002002709700002502729700001802754700002602772700002002798700002102818700003402839856012202873 2020 eng d00aElectrospun fibers and sorbents as a possible basis for effective composite wound dressings0 aElectrospun fibers and sorbents as a possible basis for effectiv cAPR a4410 v113 a

Skin burns and ulcers are considered hard-to-heal wounds due to their high infection risk. For this reason, designing new options for wound dressings is a growing need. The objective of this work is to investigate the properties of poly (epsilon-caprolactone)/poly (vinyl-pyrrolidone) (PCL/PVP) microfibers produced via electrospinning along with sorbents loaded with Argovit (TM) silver nanoparticles (Ag-Si/Al2O3) as constituent components for composite wound dressings. The physicochemical properties of the fibers and sorbents were characterized using scanning electron microscopy (SEM), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR) and inductively coupled plasma optical emission spectroscopy (ICP-OES). The mechanical properties of the fibers were also evaluated. The results of this work showed that the tested fibrous scaffolds have melting temperatures suitable for wound dressings design (58-60 degrees C). In addition, they demonstrated to be stable even after seven days in physiological solution, showing no macroscopic damage due to PVP release at the microscopic scale. Pelletized sorbents with the higher particle size demonstrated to have the best water uptake capabilities. Both, fibers and sorbents showed antimicrobial activity against Gram-negative bacteria Pseudomona aeruginosa and Escherichia coli, Gram-positive Staphylococcus aureus and the fungus Candida albicans. The best physicochemical properties were obtained with a scaffold produced with a PCL/PVP ratio of 85:15, this polymeric scaffold demonstrated the most antimicrobial activity without affecting the cell viability of human fibroblast. Pelletized Ag/Si-Al2O3-3 sorbent possessed the best water uptake capability and the higher antimicrobial activity, over time between all the sorbents tested. The combination of PCL/PVP 85:15 microfibers with the chosen Ag/Si-Al2O3-3 sorbent will be used in the following work for creation of wound dressings possessing exudate retention, biocompatibility and antimicrobial activity.

10aelectrospinning10apoly (epsilon-caprolactone)10apoly (vinyl pyrrolidone)10asilver sorbents10awound dressings1 aAlvarez-Suarez, Alan, Saul1 aDastager, Syed, G.1 aBogdanchikova, Nina1 aGrande, Daniel1 aPestryakov, Alexey1 aGarcia-Ramos, Juan, Carlos1 aPerez-Gonzalez, Graciela, Lizeth1 aJuarez-Moreno, Karla1 aToledano-Magana, Yanis1 aSmolentseva, Elena1 aPaz-Gonzalez, Juan, Antonio1 aPopova, Tatiana1 aRachkovskaya, Lyubov1 aNimaev, Vadim1 aKotlyarova, Anastasia1 aKorolev, Maksim1 aLetyagin, Andrey1 aVillarreal-Gomez, Luis, Jesus uhttps://library.ncl.res.in/content/electrospun-fibers-and-sorbents-possible-basis-effective-composite-wound-dressings