02891nas a2200241   4500008004100000022001400041245015700055210006900212260000800281300001000289490000800299520204400307653001902351653001902370653001402389653001802403653000902421653001702430100002502447700002302472700002302495856013102518       2019                        eng d  a0048-969700aBiodegradation of mixed polycyclic aromatic hydrocarbons by pure and mixed cultures of biosurfactant producing thermophilic and thermo-tolerant bacteria0 aBiodegradation of mixed polycyclic aromatic hydrocarbons by pure  cAUG  a52-600 v6793 a<p>Applicability of thermophilic and thereto-tolerant microorganisms for biodegradation of polycyclic aromatic hydrocarbons (PAHs) with low water solubility is an interesting strategy for improving the biodegradation efficiency. In this study, we evaluated utility of thermophilic and thermo-tolerant bacteria isolated from Unkeshwar hot spring (India) for biodegradation of four different PAHs. Water samples were enriched in mineral salt medium (MSM) containing a mixture of four PAHs compounds (anthracene: ANT, fluorene: FLU, phenanthrenc: PHE and pyrene: PYR) at 37 degrees C and 50 degrees C. After growth based screening, four potent strains obtained which were identified as Aeribacillus pallidus (UCPS2), Bacillus axarquiensis (UCPD1), Bacillus siamensis (GHP76) and Bacillus subtilis subsp. inaquosorum (U277) based on the 16S rRNA gene sequence analysis. Degradation of mixed PAH compounds was evaluated by pure as well as mixed cultures under shake flask conditions using MSM supplemented with 200 mg/L concentration of PAHs (50 mg/L of each compound) for 15 days at 37 degrees C and 50 degrees C. A relatively higher degradation of ANT (92% - 96%), FLU (83% - 86%), PHE (16% - 54%) and PYR (51% - 71%) was achieved at 50 degrees C by Aeribacillus sp. (UCPS2) and mixed culture. Furthermore, crude oil was used as a substrate to study the degradation of same PAHs using these organisms which also revealed with similar results with the higher degradation at 50 degrees C. Interestingly, PAH-degrading strains were also positive for biosurfactant production. Biosurfactants were identified as the variants of surfactins (lipopeptide biosurfactants) based on analytical tools and phylogenetic analysis of the surfactin genes. Overall, this study has shown that hot spring microbes may have a potential for PAHs degradation and also biosurfactant production at a higher temperature, which could provide a novel perspective for removal of PAHs residues from oil contaminated sites. (C) 2019 Elsevier B.V. All rights reserved.</p>
10aBiodegradation10aBiosurfactants10aCrude oil10aMixed culture10aPAHs10aThermophiles1 aMehetre, Gajanan, T.1 aDastager, Syed, G.1 aDharne, Mahesh, S.  uhttps://library.ncl.res.in/content/biodegradation-mixed-polycyclic-aromatic-hydrocarbons-pure-and-mixed-cultures-biosurfactant02236nas a2200265   4500008004100000022001400041245009600055210006900151260000800220300001600228490000800244520137200252653001901624653001901643653001701662653002101679653001601700100001401716700002201730700002301752700002401775700002501799700002301824856012301847       2019                        eng d  a0304-389400aNovel fatty alkene from marine bacteria: a thermo stable biosurfactant and its applications0 aNovel fatty alkene from marine bacteria a thermo stable biosurfa  cDEC  aUNSP 1208680 v3803 a<p>In this study, a novel thermo stable biosurfactants, 1-Pentanonacontene (C95H190) a fatty alkene and 3-Hydroxy16-methylheptadecanoic acid (C18H36O3) were isolated from a marine isolate SGD-AC-13. Biosurfactants were produced using 1% yeast extract in tap water as production medium at 24 h in flask and 12 h in bioreactor. Using 16S rRNA gene sequence (1515 bp) and BCL card (bioMerieux VITEK (R)), strain was identified as Bacillus sp. Crude biosurfactant reduced the surface tension of distilled water to 31.32 +/- 0.93 mN/m with CMC value of 0.3 mg/ml. Cell free supernatant showed excellent emulsification and oil displacement activity with stability up to 160 degrees C, pH 6-12 and 50 g/L NaCl conc. Biosurfactants were characterized using FTIR, TLC, HPLC LC-MS and NMR spectroscopy. Cell free supernatant reduced the contact angle of distilled water droplet from 117 degrees to 52.28 degrees and of 2% pesticide from 78.77 degrees to 73.42 degrees while 750 mu g/ml of crude biosurfactant reduced from 66.06 degrees to 56.33 degrees for 2% pesticide and recovered 35% ULO and 12% HWCO from the contaminated sand. To our best of knowledge, this is the first report of thermo stable fatty alkene as a biosurfactant and is structurally different from previously reported, with having potential application in agriculture, oil recovery and bioremediation.</p>
10aBioremediation10aBiosurfactants10aFatty alkene10aThermo stability10aWettability1 aRam, Hari1 aSahu, Amit, Kumar1 aSaid, Madhukar, S.1 aBanpurkar, Arun, G.1 aGajbhiye, Jayant, M.1 aDastager, Syed, G.  uhttps://library.ncl.res.in/content/novel-fatty-alkene-marine-bacteria-thermo-stable-biosurfactant-and-its-applications