02972nas a2200337   4500008004100000022001400041245022400055210006900279260000800348300001200356490000700368520179200375653001302167653001302180653003002193653001602223653001702239653001902256100001802275700001402293700002202307700001902329700001702348700002002365700002002385700002302405700002402428700002802452700003002480856012402510       2019                        eng d  a0723-202000aTaxonomic insights into the phylogeny of Bacillus badius and proposal for its reclassification to the genus Pseudobacillus as Pseudobacillus badius comb. nov. and reclassification of Bacillus wudalianchiensis Liu et al.0 aTaxonomic insights into the phylogeny of Bacillus badius and pro  cMAY  a360-3720 v423 a<p>The species Bacillus badius is one of the oldest members of the genus Bacillus isolated from faeces of children and was classified based on its ability to form endospores [8]. In 16S rRNA gene sequence and phylogenetic analysis, Bacillus badius DSM 23(T) shared low similarity (93.0%) and distant relationship with B. subtilis, the type species of the genus Bacillus indicating that it does not belong to this genus. Additional strains of the species, B. badius DSM 5610, DSM 30822 and B. encimensis SGD-V-25 (which has been recently reclassified as a member of this species) were included in the study to consider intraspecies diversity. Detailed molecular phylogenetic and comparative genome analysis clearly showed that the strains of B. badius were consistently retrieved outside the cluster of Bacillus sensu stricto and also distantly related to the genera Domibacillus and Quasibacillus. Further, the data from biochemical reactions (inability to ferment most carbohydrates), polar lipids profile (presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an aminophosphoglycolipid) and fatty acids supported the molecular analysis. Thus the four B. badius strains; DSM 23(T), DSM 5610, DSM 30822 and SGD-V-25 displayed sufficient demarcating phenotypic characteristics that warrant their classification as members of a novel genus and single species, for which the name Pseudobacillus badius gen. nov. comb. nov. is proposed with Pseudobacillus badius DSM 23(T) (= ATCC 14574(T)) as the type strain. Additionally, based on our findings from phenotypic, chemotaxonomic and genotypic parameters, Bacillus wudalianchiensis DSM 100757(T) was reclassified as Pseudobacillus wudalianchiensis comb. nov. (C) 2019 Elsevier GmbH. All rights reserved.</p>
10a16S rRNA10aBacillus10aBacillus wudalianchiensis10aFatty acids10apolar lipids10aPseudobacillus1 aVerma, Ashish1 aPal, Yash1 aOjha, Anup, Kumar1 aKumari, Munesh1 aKhatri, Indu1 aRameshkumar, N.1 aSchumann, Peter1 aDastager, Syed, G.1 aMayilraj, Shanmugam1 aSubramanian, Srikrishna1 aKrishnamurthi, Srinivasan  uhttps://library.ncl.res.in/content/taxonomic-insights-phylogeny-bacillus-badius-and-proposal-its-reclassification-genus02735nas a2200277   4500008004100000022001400041245006700055210006400122260000800186300000700194490000800201520192800209653001302137653001802150653002402168100001602192700002102208700001802229700001802247700001802265700002002283700001902303700001802322700002302340856009402363       2024                        eng d  a0302-893300aFictibacillus fluitans sp. nov., isolated from freshwater pond0 aFictibacillus fluitans sp nov isolated from freshwater pond  cFEB  a700 v2063 a<p>
	A Gram-positive, aerobic, rod-shaped, spore-forming bacterium, designated NE201(T), was isolated from a freshwater pond in Village Nerur, India. Growth was observed in the range of 15-45 degrees C temperature with optimum at 30 degrees C, pH range of 5-9 (optimum at 7.0), and at concentrations of NaCl ranging between 0 and 14% (optimum 0%, w/v). The 16S rRNA gene sequence showed the highest similarity with Fictibacillus enclensis NIO-1003(T) (JF893461) at 99.01% followed by F. rigui WPCB074(T) (EU939689) at 98.9% and F. solisalsi CGMCC 1.6854(T) (EU046268) at 98.66%. The digital DNA-DNA hybridization (dDDH) and orthoANI values for strain NE201(T) against F. enclensis NIO-1003(T) (GCA_900094955.1) were 33.7% and 87.68%, respectively. The phylogenetic analysis based on the 16S rRNA gene, 92 core genes derived from the genome, and 20 proteins involving over 20,236 amino acid positions revealed the distinct phylogenetic position of strain NE201(T) and the formation of a clearly defined monophyletic clade with F. enclensis. The strain NE201(T) showed a unique carbon utilization and assimilation pattern that differentiated it from F. enclensis NIO-1003(T). The major fatty acids were anteiso -C-15:0 (51.42%) and iso-C-15:0 (18.88%). The major polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE, and diphosphatidylglycerol (DPG). The antiSMASH analyzed genome of NE201(T) highlighted its diverse biosynthetic potential, unveiling regions associated with terpene, non-ribosomal peptide synthetases (NRPS), lassopeptides, NI-siderophores, lanthipeptides (LAP), and Type 3 Polyketide Synthases (T3PKS). The overall phenotypic, genotypic, and chemotaxonomic characters strongly suggested that the strain NE201(T) represents a novel species of genus Fictibacillus for which the name Fictibacillus fluitans sp. nov. is proposed. The type strain is NE201(T) (= MCC 5285 = JCM 36474).</p>
10aBacillus10aFictibacillus10aFreshwater bacteria1 aYadav, Amit1 aMaurya, Akanksha1 aBhavsar, Yash1 aTeware, Ruchi1 aBhatt, Agrima1 aDaCosta, Elaine1 aThorat, Vipool1 aKirdat, Kiran1 aDastager, Syed, G.  uhttps://library.ncl.res.in/content/fictibacillus-fluitans-sp-nov-isolated-freshwater-pond