02149nas a2200193   4500008004100000245011600041210006900157260000800226300001200234490000800246520141800254100002901672700002501701700002701726700003001753700002101783700002201804856012901826       2017                        eng d00aDiastase induced green synthesis of bilayered reduced graphene oxide and its decoration with gold nanoparticles0 aDiastase induced green synthesis of bilayered reduced graphene o  cJAN  a252-2580 v1663 aIn this paper, we report an enzyme dependent, green one-pot deoxygenation cum decoration method to synthesize diastase-conjugated reduced graphene oxide (DRG) nanosheets, DRG/gold nanoparticles (DRG/Au) composite. The DRG synthesis was completed in 7 h under heating at 90 degrees C on water bath. Selected area electron diffraction (SAED) and Atomic force microscopy (AFM) study has revealed the formation of bilayered reduced graphene oxide sheets. Transmission electron microscopy (TEM) images of DRG/Au composite have shown the uniform decoration of gold nanoparticles (AuNPs) onto the DRG nanosheet surface. Fourier transform infrared spectroscopy (FTIR) and Raman results additionally have shown the functionalization of enzyme molecules onto the DRG nanosheet surface after reduction making it as an effective platform towards the efficient binding of gold nanoparticles. In vitro cytotoxicity studies by MIT assay on A549 and HCT116 cell lines exhibited that the cytotoxicity of the prepared graphene oxide (GO), DRG and DRG/Au is dose dependant. These results have shown that this synthetic method is effective for the production of large scale graphene in a low cost, simple and green method. Since this process avoids the use of hazardous and toxic substances, the produced DRG/Au composites are likely to offer various potential applications in biology and medicine. (C) 2016 Published by Elsevier B.V1 aMaddinedi, Sireesh, Babu1 aMandal, Badal, Kumar1 aPatil, Sagar, Hindurao1 aAndhalkar, Vaibhav, Vilas1 aRanjan, Shivendu1 aDasgupta, Nandita  uhttps://library.ncl.res.in/content/diastase-induced-green-synthesis-bilayered-reduced-graphene-oxide-and-its-decoration-gold