02606nas a2200265   4500008004100000022001400041245016900055210006900224260000800293300001100301490000700312520164400319653000601963653002601969653001801995653003102013653002802044100002502072700002802097700002202125700002202147700001902169700002302188856012902211       2022                        eng d  a1018-364700a2,4-Di-tert-butylphenol (2,4-DTBP) purified from streptomyces sp. KCA1 from phyllanthus niruri: isolation, characterization, antibacterial and anticancer properties0 a24Ditertbutylphenol 24DTBP purified from streptomyces sp KCA1 fr  cJUL  a1020880 v343 a<p>
	Objectives: The present study reports the bioactivity of 2,4-Di-tert-butylphenol (2,4-DTBP) purified from an endophytic Streptomyces species KCA1 isolated from the leaves of Phyllanthus niruri and its antibacterial and anticancer properties. Methods: The extracellular metabolites were produced from the strain KCA1 by submerged fermentation using ethyl acetate. The crude extract was further evaluated for antibacterial activity against set of pathogens. Active metabolite from the extract was purified using chromatography techniques and detected its antibacterial activity by disc diffusion assay. The molecular structure of the active molecule was identified through various spectral study. Moreover, the bioactive metabolite 2,4-DTBP was analyzed antibacterial and anti-proliferative activity. Results: Strain KCA1 was identified as Streptomyces sp. In the preliminary screening, the crude extract exhibited broad spectrum activity against various bacterial pathogens. Based on the spectral properties, the active metabolite was identified as 2,4-Di-tert-butylphenol. The MIC of active compound 2,4-DTBP inhibited E. coli ATCC 25922 and S. aureus ATCC 29213 at 50 lg/ml and 0.78 lg/ml, respectively. The IC50 value of 2,4-DTBP was found to be 11.0 lg/ml and 116.8 lg/ml, against breast cancer cell line (MCF7) and normal VERO cell line, respectively. Conclusions: This study concluded that 2,4-DTBP, produced from the endophytic Streptomyces sp. KCA1, is the potential candidate to develop as promising antibacterial and anticancer agent. (C) 2022 The Authors. Published by Elsevier B.V. on behalf of King Saud University.</p>
10a210a4-Di-tert-butylphenol10aAntibacterial10aantiproliferative activity10aEndophytic Streptomyces1 aSeenivasan, Ayswarya1 aManikkam, Radhakrishnan1 aKaari, Manigundan1 aSahu, Amit, Kumar1 aSaid, Madhukar1 aDastager, Syed, G.  uhttps://library.ncl.res.in/content/24-di-tert-butylphenol-24-dtbp-purified-streptomyces-sp-kca1-phyllanthus-niruri-isolation02733nas a2200349   4500008004100000022001400041245012900055210006900184260000800253300001400261490000800275520154800283653001901831653002001850653001301870653001801883653002601901100002801927700002201955700002801977700002202005700002202027700001902049700002702068700002202095700002902117700003802146700002302184700002702207700001802234856013102252       2023                        eng d  a0273-228900aAntibacterial and anti-HIV metabolites from marine streptomyces albus MAB56 isolated from Andaman and Nicobar Islands, India0 aAntibacterial and antiHIV metabolites from marine streptomyces a  cDEC  a7738-77540 v1953 a<p>
	Marine-derived actinobacteria have tremendous potential to produce novel metabolites with diverse biological activities. The Andaman coast of India has a lot of microbial diversity, but it is still a relatively unknown ecology for isolating novel actinobacteria with beneficial bioactive compounds. We have isolated 568 actinobacterial strains from mangrove rhizosphere sediments and sponge samples. Crude extracts from 75 distinct strains were produced by agar surface fermentation and extracted using ethyl acetate. In the disc diffusion method, 25 actinobacterial strains showed antimicrobial activity; notably, the strain MAB56 demonstrated promising broad-spectrum activity. Strain MAB56 was identified as Streptomyces albus by cultural, microscopic, and molecular methods. Conditions for bioactive metabolites from MAB56 were optimized and produced in a lab-scale fermenter. Three active metabolites (C1, C2, and C3) that showed promising broad-spectrum antimicrobial activity were isolated through HPLC-based purification. Based on the UV, FT-IR, NMR, and LC-MS analysis, the chemical nature of the active compounds was confirmed as 12-methyltetradecanoic acid (C1), palmitic acid (C2), and tridecanoic acid (C3) with molecular formulae C14H28O2, C16H32O2, and C13H26O2, respectively. Interestingly, palmitic acid (C2) also exhibited anti-HIV activity with an IC50 value of &lt; 1 mu g/ml. Our findings reveal that the actinobacteria from the Andaman marine ecosystems are promising for isolating anti-infective metabolites.</p>
10aactinobacteria10aAndaman Islands10aanti-HIV10aAntibacterial10aBioactive metabolites1 aManikkam, Radhakrishnan1 aMurthy, Sangeetha1 aPalaniappan, Sivasankar1 aKaari, Manigundan1 aSahu, Amit, Kumar1 aSaid, Madhukar1 aGanesan, Vijayalakshmi1 aKannan, Sivakumar1 aRamasamy, Balagurunathan1 aThirugnanasambandan, Somasundaram1 aDastager, Syed, G.1 aHanna, Luke, Elizabeth1 aKumar, Vanaja  uhttps://library.ncl.res.in/content/antibacterial-and-anti-hiv-metabolites-marine-streptomyces-albus-mab56-isolated-andaman-and