01720nas a2200217   4500008004100000245009800041210006900139260000800208300001400216490000700230520096100237100001801198700002201216700002101238700002901259700001901288700002301307700002101330700002401351856012701375       2017                        eng d00aAcetone and methanol fruit extracts of terminalia paniculata inhibit HIV-1 infection in vitro0 aAcetone and methanol fruit extracts of terminalia paniculata inh  cJAN  a1468-14710 v313 aIn this study, we report the in vitro anti-HIV1 activity of acetone and methanol extracts of fruit of Terminalia paniculata. Cytotoxicity tests were conducted on TZM-bl cells and peripheral blood mononuclear cells (PBMC), the CC50 values of both the extracts were 260g/mL. Using TZM-bl cells, the extracts were tested for their ability to inhibit replication of two primary isolates HIV-1 (X4, Subtype D) and HIV-1 (R5, Subtype C). The activity against HIV-1 primary isolate (R5, Subtype C) was confirmed using activated PBMC and by quantification of HIV-1 p24 antigen. Both the extracts showed anti-HIV1 activity in a dose-dependent manner. The EC50 values of the acetone and methanol extracts of T. paniculata were 10.3g/mL. The enzymatic assays were performed to determine the mechanism of action which indicated that the anti-HIV1 activity might be due to inhibition of reverse transcriptase (77.7% inhibition) and protease (69.9% inhibition) enzymes. 1 aDurge, Ankita1 aJadaun, Pratiksha1 aWadhwani, Ashish1 aChinchansure, Ashish, A.1 aSaid, Madhukar1 aThulasiram, H., V.1 aJoshi, Swati, P.1 aKulkarni, Smita, S.  uhttps://library.ncl.res.in/content/acetone-and-methanol-fruit-extracts-terminalia-paniculata-inhibit-hiv-1-infection-vitro02778nas a2200325   4500008004100000245012400041210006900165260000800234300000700242490000700249520169500256653002401951653002001975653002701995653002302022653001702045653001402062653002002076653002802096100002202124700002202146700002202168700002302190700002602213700001902239700002202258700001802280700002202298856013202320       2023                        eng d00aAntiviral and ROS scavenging potential of Carica papaya Linn and Psidium guajava leaves extract against HIV-1 infection0 aAntiviral and ROS scavenging potential of Carica papaya Linn and  cMAR  a820 v233 a<p>
	Antiretroviral therapy is the only treatment option for HIV-infected patients; however, it has certain drawbacks in terms of developing multiple toxic side effects. Thus, there is a continuous need to explore safe and efficacious anti-retroviral agents. Carica papaya Linn and Psidium guajava are known for their various biological activities. In this study, we characterized the bioactive fractions of methanolic leaves extract from both plants using the High-resolution electrospray ionization mass spectrometry (HR-ESI-MS) technique, followed by the investigation of their potential as anti-HIV-1 and antioxidant agents through in vitro mechanistic assays. The anti-HIV-1 activity was examined in TZM-bl cells through luciferase gene assay against two different clades of HIV-1 strains, whereas the intracellular ROS generation was analyzed by Fluorescence-Activated Cell Sorting. Additionally, the mechanisms of action of these phyto-extracts were determined through the Time-of-addition assay. The characterization of Carica papaya Linn and Psidium guajava leaves extract through HR-ESI-MS fragmentation showed high enrichment of various alkaloids, glycosides, lipids, phenolic compounds, terpenes, and fatty acids like bioactive constituents. Both the phyto-extracts were found to be less toxic and exhibited potent antiviral activity against HIV-1 strains. Furthermore, the phyto-extracts also showed a decreased intracellular ROS in HIV-1 infected cells due to their high antioxidant potential. Overall, our study suggests the anti-HIV-1 potential of Carica papaya Linn and Psidium guajava leaves extract due to the synergistic action of multiple bioactive constituents.</p>
10aanti-HIV-1 activity10aAnti-retroviral10aBioactive constituents10aCarica papaya Linn10aCytotoxicity10aHR-ESI-MS10aPsidium guajava10areactive oxygen species1 aJadaun, Pratiksha1 aShah, Prachibahen1 aHarshithkumar, R.1 aSaid, Madhukar, S.1 aBhoite, Shubhangi, P.1 aBokuri, Sowmya1 aRavindran, Selvan1 aMishra, Neetu1 aMukherjee, Anupam  uhttps://library.ncl.res.in/content/antiviral-and-ros-scavenging-potential-carica-papaya-linn-and-psidium-guajava-leaves-extract