02670nas a2200205 4500008004100000245010900041210006900150260000800219300002300227490000700250520191300257100002902170700001702199700002402216700002402240700002802264700002002292700002402312856012802336 2017 eng d00aDraft genome sequence of lactobacillus plantarum strains E2C2 and E2C5 isolated from human stool culture0 aDraft genome sequence of lactobacillus plantarum strains E2C2 an cJAN aArticle Number: 150 v123 aProbiotic Lactobacillus species offer various health benefits, thus have been employed in treatment and prevention of various diseases. Due to the differences in the isolation source and the site of action, most of the lactobacilli tested in-vitro for probiotics properties fail to extend similar effects in-vivo. Consequently, the search of autochthonous, efficacious and probably population specific probiotics is a high priority in the probiotics research. In this regards, whole genome sequencing of as many Lactobacillus as possible will help to deepen our understanding of biology and their health effects. Here, we provide the genomic insights of two coherent oxalic acid tolerant Lactobacillus species (E2C2 and E2C5) isolated from two different healthy human gut flora. These two isolates were found to have higher tolerance towards oxalic acid (300 mM sodium oxalate). The draft genome of strain E2C2 consists of 3,603,563 bp with 3289 protein-coding genes, 94 RNA genes, and 43.99% GC content, while E2C5 contained 3,615,168 bp, 3293 coding genes (93.4% of the total genes), 95 RNA genes and 43.97% GC content. Based on 16S rRNA gene sequence analysis followed by in silico DNA-DNA hybridization studies, both the strains were identified as Lactobacillus plantarum belonging to family Lactobacillaceae within the phylum Firmicutes. Both the strains were genomically identical, sharing 99.99% CDS that showed 112 SNPs. Both the strains also exhibited deconjugation activity for the bile salts while genome analysis revealed that the L. plantarum strains E2C2 and E2C5 also have the ability to produce vitamins, biotin, alpha-and beta-glucosidase suggesting potential probiotic activities of the isolates. The description presented here is based on the draft genomes of strains E2C2 and E2C5 which are submitted to GenBank under the accession numbers LSST00000000.1 and LTCD00000000.1, respectively.1 aSuryavanshi, Mangesh, V.1 aPaul, Dhiraj1 aDoijad, Swapnil, P.1 aBhute, Shrikant, S.1 aHingamire, Tejashri, B.1 aGune, Rahul, P.1 aShouche, Yogesh, S. uhttps://library.ncl.res.in/content/draft-genome-sequence-lactobacillus-plantarum-strains-e2c2-and-e2c5-isolated-human-stool02265nas a2200277 4500008004100000022001400041245008300055210006900138260000800207300001100215490000800226520139500234653002801629653001501657653002201672653002701694653003101721100001801752700001801770700001701788700002001805700001801825700001601843700001701859856011101876 2020 eng d a0269-749100aCharacterisation of hyper tolerant Bacillus firmus L-148 for arsenic oxidation0 aCharacterisation of hyper tolerant Bacillus firmus L148 for arse cJUN a1141240 v2613 a

Groundwater arsenic pollution causes millions of deaths worldwide. Long term natural and anthropogenic activities have increased arsenic levels in groundwater causing higher threats of arsenic exposure. Arsenic hyper-tolerant Firmicute Bacillus firmus L-148 was isolated from arsenic limiting Lonar lake soil, which tolerated more than 3 M arsenic and could oxidize 75 mM arsenite [As(III)] in 14 days. It oxidized As(III) in presence of heavy metals and had unusual pH optima at 9.2. B. firmus L-148 was studied at the biochemical, protein, genomic and transcript level for understanding its arsenic oxidizing machinery. The proteomic and transcript analysis exhibited the presence of ars and aio operon and supported the inducible nature of ars operon. Robust, hyper-tolerant, fast As(III) oxidizing, least nutrient requiring and multi-metal resistance qualities of the strain were used in microcosm studies for bioremediation. Artificial groundwater mimicking microcosm with 75 mM As(III) was developed. Modulation of carbon source, iron and multi metals affected growth and As(III) oxidation rate. The As(III) oxidation was recorded to be 77% in 15 days in presence of sodium acetate and Fe ions. This microcosm study can be explored for bioremediation of arsenic contaminated water and followed by precipitation using other methods. (C) 2020 Elsevier Ltd. All rights reserved.

10aArsenic hyper-tolerance10aLonar lake10aMicrocosm studies10aMulti-metal resistance10aNext generation sequencing1 aBagade, Aditi1 aNandre, Vinod1 aPaul, Dhiraj1 aPatil, Yugendra1 aSharma, Nisha1 aGiri, Ashok1 aKodam, Kisan uhttps://library.ncl.res.in/content/characterisation-hyper-tolerant-bacillus-firmus-l-148-arsenic-oxidation02726nas a2200277 4500008004100000022001400041245011000055210006900165260000800234300001100242490000800253520182900261653001502090653002402105653001502129653001702144653001602161653000902177100002102186700002502207700001702232700002302249700002302272700002702295856012602322 2022 eng d a0944-501300aExploring the core microbiota in scented rice (Oryza sativa L.) rhizosphere through metagenomics approach0 aExploring the core microbiota in scented rice Oryza sativa L rhi cOCT a1271570 v2633 a

Rice is a major food crop cultivated around the globe. Specially scented rice varieties are of commercial importance but they are low-yielding. The rhizospheric microflora plays a significant role in improving yield and aroma. However, the core microbiome of the scented rice rhizosphere is comparatively less explored. Here, we analyzed the core microbiome associated with the rhizosphere of the scented (Ambemohar-157 and Dehradun basmati) in comparison with non-scented rice (Kolam and Arize 6444 Gold) cultivated at two different geoclimatic zones of India (Maharashtra and Uttarakhand) using the metagenomics approach. The alpha and beta diversity analysis showed that the microbial communities associated with scented and non-scented varieties significantly changes with respect to richness, diversity, and evenness. The taxonomic profiling revealed the variation in composition, diversity, and abundance of the microbiome in terms of phyla and genera associated with scented rice varieties over non-scented. The cluster analysis distinguishes the microbial communities based on their geographical positions. The core microbiome analysis revealed that scented rice rhizosphere shelters distinct and unique microbiota. 28.6 % of genera were exclusively present only in the scented rice rhizosphere. The putative functional gene annotation revealed the high abundance of genes related to the biosynthesis of 2-acetyl-1-pyrroline (2AP) precursors in scented rice. The precursor feeding analysis revealed proline as a preferred substrate by 2AP synthesizing bacteria. The 2AP precursor proline and proline metabolism genes showed a positive correlation. The scented rice-specific rhizobacteria pointed out in this study can be used as bioinoculants for enhancing aroma, yield, and sustainable rice cultivation.

10aMetagenome10aMicrobial community10aMicrobiome10aOryza sativa10aRhizosphere10arice1 aDhondge, Harshal1 aBarvkar, Vitthal, T.1 aPaul, Dhiraj1 aDastager, Syed, G.1 aPable, Anupama, A.1 aNadaf, Altafhusain, B. uhttps://library.ncl.res.in/content/exploring-core-microbiota-scented-rice-oryza-sativa-l-rhizosphere-through-metagenomics02611nas a2200241 4500008004100000245014200041210006900183260000800252300001100260490000700271520174900278653002202027653002402049653002102073653001802094653001602112100002802128700002102156700001702177700002402194700002402218856012702242 2023 eng d00aSeasonal dynamics and tree foliar habit drive the rhizobacterial diversity in congeneric Ficus species from Northern Western Ghats, India0 aSeasonal dynamics and tree foliar habit drive the rhizobacterial cDEC a1007900 v283 a

Tree species, annual climatic cycle, and geochemical properties influence the tree rhizospheric microbiome. However, its understanding in the moist deciduous forest of Northern Western Ghats (NWG), India, is limited. Moreover, the effects of foliar habits of trees and seasonal variations on rhizobacterial communities across congeneric tree species are unexplored. Hence, we employed high throughput 16 S rRNA gene sequencing to examine the effects of foliar habits of congeneric tree species and seasonal influences on the rhizobacterial community. For this study, rhizospheric soil from Ficus arnottiana (deciduous) and Ficus talboti (evergreen) trees from NWG, India, was collected during three seasons (summer, monsoon, and winter) and subjected to high throughput sequencing. A total of 3031 amplicon sequence variants (ASV) were observed, of which 1257 and 1181 ASV were unique to F. arnottiana and F. talboti, respectively, and 593 ASV were common to both species. The phyla Actinobacteria, Proteobacteria, Acidobacteria, and Chloroflexi were ubiquitous and contributed >90% to the rhizobacterial community. Some bacterial phyla were exclusively associated with either of the tree species and seasons. Different bacterial phyla performed similar functions in different seasons, indicating their functional redundancy and emphasizing their resilience and adaptability across seasons. It was observed that seasonal dynamics mainly drove the rhizobacterial diversity; however, it was also influenced by the foliar habits of the trees and geochemical properties. The study enhances the understanding of congeneric tree microbiomes and offers valuable insights into their potential applications through microbiome engineering.

10aAmplicon variants10aBacterial diversity10aFicus arnottiana10aFicus talboti10aRhizosphere1 aSalunkhe, Vaishnavi, H.1 aKunte, Pooja, S.1 aPaul, Dhiraj1 aKasodekar, Amol, K.1 aKadoo, Narendra, Y. uhttps://library.ncl.res.in/content/seasonal-dynamics-and-tree-foliar-habit-drive-rhizobacterial-diversity-congeneric-ficus