03076nas a2200265   4500008004100000022001400041245011900055210006900174260000800243300001400251490000600265520217000271100002302441700002202464700002402486700002002510700002002530700001902550700002202569700002102591700003702612700001802649700002002667856012302687       2019                        eng d  a2470-134300aAzadirachtin-A from azadirachta indica impacts multiple biological targets in cotton bollworm helicoverpa armigera0 aAzadirachtinA from azadirachta indica impacts multiple biologica  cMAY  a9531-95410 v43 a<p>Azadirachtin-A (AzaA) from the Indian neem tree (Azadirachta indica) has insecticidal properties; however, its molecular mechanism remains elusive. The ``targeted and nontargeted proteomic profiling'', metabolomics, matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) imaging, gene expression, and in silico analysis provided clues about its action on Helicoverpa armigera. Fourth instar H. armigera larvae fed on AzaA-based diet (AzaD) suffered from significant mortality, growth retardation, reduced larval mass, complications in molting, and prolonged development. Furthermore, death of AzaD-fed larvae was observed with various phenotypes like bursting, blackening, and half-molting. Liquid chromatography-mass spectrometry (LC-MS) data showed limited catabolic processing of ingested AzaA and dramatic alternations of primary metabolism in H. armigera. MALDI-TOF imaging indicated the presence of AzaA in midgut of H. armigera. In the gut, out of 79 proteins identified, 34 were upregulated, which were related to digestion, immunity, energy production, and apoptosis mechanism. On the other hand, 45 proteins were downregulated, including those from carbohydrate metabolism, lipid metabolism, and energy transfer. In the hemolymph, 21 upregulated proteins were reported to be involved in immunity, RNA processing, and mRNA-directed protein synthesis, while 7 downregulated proteins were implicated in energy transfer, hydrolysis, lipid metabolism, defense mechanisms, and amino acid storage-related functions. Subsequently, six target proteins were identified using labeled AzaA that interacted with whole insect proteins. In silico analysis suggests that AzaA could be efficiently accommodated in the hydrophobic pocket of juvenile hormone esterase and showed strong interaction with active site residues, indicating plausible targets of AzaA in H. armigera. Quantitative polymerase chain reaction analysis suggested differential gene expression patterns and partly corroborated the proteomic results. Overall, data suggest that AzaA generally targets more than one protein in H. armigera and hence could be a potent biopesticide.</p>
1 aDawkar, Vishal, V.1 aBarage, Sagar, H.1 aBarbole, Ranjit, S.1 aFatangare, Amol1 aGrimalt, Susana1 aHaldar, Saikat1 aHeckel, David, G.1 aGupta, Vidya, S.1 aThulasiram, Hirekodathakallu, V.1 aSvatos, Ales.1 aGiri, Ashok, P.  uhttps://library.ncl.res.in/content/azadirachtin-azadirachta-indica-impacts-multiple-biological-targets-cotton-bollworm01182nas a2200169   4500008004100000245014600041210006900187260000900256490000700265520050900272100002400781700002000805700001800825700002000843700002200863856012700885       2024                        eng d00aArticle chitinase inhibition induces transcriptional dysregulation altering ecdysteroid-mediated control of spodoptera frugiperda development0 aArticle chitinase inhibition induces transcriptional dysregulati  cMAR 0 v273 a<p>
	Chitinases and ecdysteroid hormones are vital for insect development. Crosstalk between chitin and ecdysteroid metabolism regulation is enigmatic. Here, we examined chitinase inhibition effect on Spodopsilencing and overexpression resulted in ecdysone receptor deregulation. Transcription factors, like Neverland, and other ecdysteroid biosynthesis genes might lead to their upregulation in berberine-fed chitinase activity's impact on ecdysone biosynthesis and its transcriptional crosstalk.</p>
1 aBarbole, Ranjit, S.1 aSharma, Shivani1 aPatil, Yogita1 aGiri, Ashok, P.1 aJoshi, Rakesh, S.  uhttps://library.ncl.res.in/content/article-chitinase-inhibition-induces-transcriptional-dysregulation-altering-ecdysteroid