02016nas a2200229 4500008004100000022001400041245009600055210006900151260009900220300001400319490000700333520110200340653004001442653002301482653003301505653002901538100002201567700003001589700002301619700002001642856012401662 2007 eng d a0960-852400aStrain improvement of penicillium janthinellum ncim 1171 for increased cellulase production0 aStrain improvement of penicillium janthinellum ncim 1171 for inc aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcMAY a1467-14730 v983 a

The strain of Penicilhuinjanthinellunt NOM 1171 was subjected to mutation involving treatment of Ethyl Methyl Sulfonate (EMS) for 24 h followed by UV-irradiation for 3 min. Successive mutants showed enhanced cellulase production (EMS-UV-8), clearance zone on Avicel containing plate (SM2) and rapid growth on Walseth cellulose agar plates containing 0.2% 2-deoxy-D-glucose (SM3). These mutants were transferred to Walseth cellulose plates containing higher concentration (1.5%) of 2-deoxy-D-glucose (SM4) in which only five mutants showed clearance zone on SM4. All these mutants showed approximately two-fold increase in activity of both FPase and CMCase in shake flask culture when grown on basal medium containing CP-123 (1%) and wheat bran (2.5%). The enzyme preparations from these mutants were used to hydrolyze Avicel. Higher hydrolysis yields of Avicel were obtained with enzyme preparations of EU1. This is the first report on the isolation and selection of mutants based on hydrolysis of Avicel, which is the most crystalline substrate. (c) 2006 Elsevier Ltd. All rights reserved.

10a2-deoxy-D-glucose resistant mutants10acellulase activity10aEMS and ultraviolet mutation10aPenicillium janthinellum1 aAdsul, Mukund, G.1 aBastawade, Kulbhushan, B.1 aVarma, Anjanikumar1 aGokhale, D., V. uhttps://library.ncl.res.in/content/strain-improvement-penicillium-janthinellum-ncim-1171-increased-cellulase-production02747nas a2200229 4500008004100000022001400041245020500055210006900260260009900329300001200428490000700440520176600447653002702213653003702240653001202277653001902289653002902308100001602337700001502353700002302368856012602391 2007 eng d a0144-861700aSugar-linked biodegradable polymers: regio-specific ester bonds of glucose hydroxyls in their reaction with maleic anhydride functionalized polystyrene and elucidation of the polymer structures formed0 aSugarlinked biodegradable polymers regiospecific ester bonds of  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcFEB a576-5850 v673 a

In the development of sugar-linked synthetic polymers as biodegradable polymers, it is imperative to know the variety of polymer structures formed by the reaction of a multi-functional sugar molecule with the functionalized synthetic polymer on which the sugar is to be anchored. Enzymes produced by the microorganisms causing the polymer to biodegrade can be sensitive to the particular type of sugar hydroxyl utilized (such as anomeric, primary, or secondary hydroxyl group) for getting anchored to the polymer. In this paper, we present synthesis of regio-specific ester derivatives of glucose with anhydride, functionalized polymers, i.e., ester formation specifically with the anomeric, primary or secondary hydroxyls of glucose. Characterization of these different esters groups was done using FTIR spectroscopy; each ester peak was further deconvoluted to yield its different components. For this purpose, we studied the reactions of D-glucose, 6-O-trityl glucose, methyl glucoside, 1,2-5,6-diisopropylidene-D-glucose, and 1,2,3,4-tetraacetyl-D-glucose with maleic anhydride functionalized polystyrene (PSMAH). In this study, the primary hydroxyl of glucose was found to be even more reactive than the anomeric hydroxyl. The peaks at similar to 1716, similar to 1725, and 1729-1737 cm(-1) were assigned to the ester carbonyl of the anomeric, primary, and secondary hydroxyls of glucose (C2, C3, and C4), respectively. An attempt was made to quantify the extent to which the different polymer structures are formed in a particular reaction by taking ratios of non-variable reference peaks (polystyrene peak at 1493 cm-1) and variable peaks caused by the reaction (the residual anhydride carbonyl at 1780 cm(-1)). (c) 2007 Published by Elsevier Ltd.

10aBiodegradable polymers10afunctionalized synthetic polymer10aglucose10aregio-specific10asugar-linked polystyrene1 aGalgali, P.1 aAgashe, M.1 aVarma, Anjanikumar uhttps://library.ncl.res.in/content/sugar-linked-biodegradable-polymers-regio-specific-ester-bonds-glucose-hydroxyls-their02266nas a2200229 4500008004100000022001400041245015400055210006900209260009900278300001200377490000800389520136000397653002101757653001401778653001801792653000801810653001001818100002501828700003501853700002301888856012501911 2014 eng d a0144-861700aSupramolecular transitions in native cellulose-I during progressive oxidation reaction leading to quasi-spherical nanoparticles of 6-carboxycellulose0 aSupramolecular transitions in native celluloseI during progressi aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcNOV a615-6230 v1133 a

Cellulose-I swells considerably in phosphoric acid, and converts to amorphous cellulose via a cellulose-II transition state. Controlled oxidation of cellulose-I to 6-carboxycellulose (6CC) using HNO3-H3PO4-NaNO2 oxidation system led to the selective production of 6CC's of varying carboxyl contents (1.7-22%) as well as various shapes and sizes (macro-sized fibrils of several micron length and/or spherical nanoparticles of 25-35 nm), depending on the reaction conditions. 6CC's having less than 14% carboxyl content were largely in cellulose-II form (WAXRD values in-between cellulose I and cellulose II), whereas at 14-22% the 6CC's were largely amorphous; only trace crystallinity was observed at 19% and 22% carboxyl 6CC. Spherical nanoparticles retained a high degree of crystallinity having cellulose-I structure, whereas the macro-sized fibrils were largely converted to cellulose-II structure. Analysis by WAXRD as well as by CP-MAS C-13 NMR studies gave similar conclusions. Reduced molecular weight with progressive oxidation, including presence of oligomers, was also evident from an increase in the reducing-end carbon peak at similar to 92 ppm. For high oxidation levels (>14%) the NMR 92-96 ppm peaks disappeared on extracting with dilute alkali, due to soluble oligomers being removed. (C) 2014 Elsevier Ltd. All rights reserved.

10aCarboxycellulose10acellulose10aNanoparticles10aNMR10aWAXRD1 aSharma, Priyanka, R.1 aRajamohanan, Pattuparambil, R.1 aVarma, Anjanikumar uhttps://library.ncl.res.in/content/supramolecular-transitions-native-cellulose-i-during-progressive-oxidation-reaction-002057nas a2200241 4500008004100000022001400041245009800055210006900153260007300222300001200295490000700307520118200314653001901496653001401515653002801529653002401557100002501581700001801606700001901624700001901643700002301662856013001685 2016 eng d a0141-813000aShape and size engineered cellulosic nanomaterials as broad spectrum anti-microbial compounds0 aShape and size engineered cellulosic nanomaterials as broad spec aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJUN a460-4650 v873 a

Oxidized celluloses have been used for decades as antimicrobial wound gauzes and surgical cotton. We now report the successful synthesis of a next generation narrow size range (25-35 nm) spherical shaped nanoparticles of 2,3,6-tricarboxycellulose based on cellulose I structural features, for applications as new antimicrobial materials. This study adds to our previous study of 6-carboxycellulose. A wide range of bacteria such as Escherichia coli, Staphloccocus aureus, Bacillus subtilis and Mycobacterium tuberculosis (non-pathogenic as well as pathogenic strains) were affected by these polymers in in vitro studies. Activity against Mycobacteria were noted at high concentrations (MIC99 values 250-1000 mu g/ml, as compared to anti-TB drug Isoniazid 0.3 mu g/ml). However, the broad spectrum activity of oxidized celluloses and their nanoparticles against a wide range of bacteria, including Mycobacteria, show that these materials are promising new biocompatible and biodegradable drug delivery vehicles wherein they can play the dual role of being a drug encapsulant as well as a broad spectrum anti-microbial and anti-TB drug. (C) 2016 Published by Elsevier B.V.

10aAnti-microbial10acellulose10aSpherical nanoparticles10aTricarboxycellulose1 aSharma, Priyanka, R.1 aKamble, Sunil1 aSarkar, Dhiman1 aAnand, Amitesh1 aVarma, Anjanikumar uhttps://library.ncl.res.in/content/shape-and-size-engineered-cellulosic-nanomaterials-broad-spectrum-anti-microbial-compounds