02773nas a2200301   4500008004100000022001400041245006400055210006400119260009900183300000900282490000700291520177900298653002802077653001402105653002302119653002502142653002902167653002202196653001302218100002202231700001802253700001502271700001402286700003002300700002002330700002302350856009802373       2005                        eng d  a0144-861700aEnzymatic hydrolysis of delignified bagasse polysaccharides0 aEnzymatic hydrolysis of delignified bagasse polysaccharides  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcOCT  a6-100 v623 a<p>Sugarcane bagasse, consisting of cellulose, xylan, and lignin, was chemically treated to generate bagasse samples with continuously decreasing content of lignin. These bagasse samples were hydrolyzed by cellulase and xylanase enzymes, produced earlier by Penicillium janthinellum NCIM 1171 in the same bagasse polysaccharides production medium. The hydrolysis was carried out by using different concentrations of the enzymes at two different temperatures, 30 and 50 degrees C, taking hydrolysis of Avicel as control. It was found that while the maximum hydrolysis for Avicel was 70% that of some of the bagasse polysaccharides was as high as 95%. The products of hydrolysis were glucose, xylose, and arabinose, as confirmed by high pressure ion chromatography (HPIC). It is interesting to note that arabinose, which constitutes about 10% of the weight of bagasse xylan, could also be released easily by the enzymes. Also, the initial rates of hydrolysis was found to be much higher for the bagasse polysaccharides, and in some cases about 90% of the hydrolysis occurred within 20 h. Amongst all bagasse samples, the sample with (Kappa no. 1.2, lignin content 0.18%) gave the highest degree of hydrolysis at 50 degrees C. Even the bagasse polysaccharide with Kappa no. 16.8 (lignin content 2.5%) underwent greater extent of hydrolysis than Avicel. Apparently, the delignified bagasse medium appears to be a facile medium for the combined hydrolytic action of the cellulase and xylanase enzymes. Considering that sugarcane bagasse is a waste biomass material available in abundance annually, this methodology can be used to value-add to this biomass to produce sugars, which can be fermented to produce biofuels like ethanol. (C) 2005 Elsevier Ltd. All rights reserved.</p>10abagasse polysaccharides10aCellulase10achemical treatment10aEnzymatic hydrolysis10aPenicillium janthinellum10aSugarcane bagasse10aXylanase1 aAdsul, Mukund, G.1 aGhule, J., E.1 aShaikh, H.1 aSingh, R.1 aBastawade, Kulbhushan, B.1 aGokhale, D., V.1 aVarma, Anjanikumar  uhttps://library.ncl.res.in/content/enzymatic-hydrolysis-delignified-bagasse-polysaccharides-002016nas a2200229   4500008004100000022001400041245009600055210006900151260009900220300001400319490000700333520110200340653004001442653002301482653003301505653002901538100002201567700003001589700002301619700002001642856012401662       2007                        eng d  a0960-852400aStrain improvement of penicillium janthinellum ncim 1171 for increased cellulase production0 aStrain improvement of penicillium janthinellum ncim 1171 for inc  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcMAY  a1467-14730 v983 a<p>The strain of Penicilhuinjanthinellunt NOM 1171 was subjected to mutation involving treatment of Ethyl Methyl Sulfonate (EMS) for 24 h followed by UV-irradiation for 3 min. Successive mutants showed enhanced cellulase production (EMS-UV-8), clearance zone on Avicel containing plate (SM2) and rapid growth on Walseth cellulose agar plates containing 0.2% 2-deoxy-D-glucose (SM3). These mutants were transferred to Walseth cellulose plates containing higher concentration (1.5%) of 2-deoxy-D-glucose (SM4) in which only five mutants showed clearance zone on SM4. All these mutants showed approximately two-fold increase in activity of both FPase and CMCase in shake flask culture when grown on basal medium containing CP-123 (1%) and wheat bran (2.5%). The enzyme preparations from these mutants were used to hydrolyze Avicel. Higher hydrolysis yields of Avicel were obtained with enzyme preparations of EU1. This is the first report on the isolation and selection of mutants based on hydrolysis of Avicel, which is the most crystalline substrate. (c) 2006 Elsevier Ltd. All rights reserved.</p>10a2-deoxy-D-glucose resistant mutants10acellulase activity10aEMS and ultraviolet mutation10aPenicillium janthinellum1 aAdsul, Mukund, G.1 aBastawade, Kulbhushan, B.1 aVarma, Anjanikumar1 aGokhale, D., V.  uhttps://library.ncl.res.in/content/strain-improvement-penicillium-janthinellum-ncim-1171-increased-cellulase-production01496nas a2200229   4500008004100000022001400041245010300055210006900158260010200227300001400329490000600343520060500349653002400954653001800978653003300996653002901029653001301058100001401071700002301085700002601108856013201134       2010                        eng d  a1930-212600aProduction of single cell protein, essential amino acids, and xylanase by penicillium janthinellum0 aProduction of single cell protein essential amino acids and xyla  aCAMPUS BOX 8005, RALEIGH, NC 27695-8005 USAbNORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCIcOCT  a2470-24770 v53 a<p>Microbial biomass having 46% crude protein content and enriched with essential amino acids as well as extracellular xylanase activity (100-150 IU/ml) was produced by an efficient fungal strain, Penicillium janthinellum (NCIM St-F-3b). Optimization studies for maximum xylanase and biomass production showed that the fungus required a simple medium containing bagasse hemicellulose as carbon source and ammonium sulphate as the nitrogen source. Therefore bagasse, which is a waste product of the sugar industry, can be efficiently used in microbioal biomass protein preparation for animal feed.</p>10aBagasse hydrolysate10aCrude protein10aMicrobial biomass production10aPenicillium janthinellum10aXylanase1 aRao, Mala1 aVarma, Anjanikumar1 aDeshmukh, Sumedha, S.  uhttps://library.ncl.res.in/content/production-single-cell-protein-essential-amino-acids-and-xylanase-penicillium-janthinellum-0