02554nas a2200253   4500008004100000022001400041245009700055210006900152260009200221300001200313490000700325520164600332100002801978700001902006700001602025700001202041700001802053700001802071700002302089700002002112700001602132700002602148856012602174       2015                        eng d  a0021-882000aNonomuraea indica sp nov., novel actinomycetes isolated from lime-stone open pit mine, India0 aNonomuraea indica sp nov novel actinomycetes isolated from limes  a2 20 8 KAMIOSAKI SHINAGAWA KU, TOKYO, 141, JAPANbJAPAN ANTIBIOTICS RESEARCH ASSOCcAUG  a491-4950 v683 a<p>A Gram-positive, aerobic, nonmotile actinomycete strain designated DRQ-2(T) was isolated from the soil sample collected from limestone open pit mine from the Gulbarga region, Karnataka province, India. Strain DRQ-2(T) was identified as a member of the genus Nonomuraea by a polyphasic approach. Strain DRQ-2(T) could be differentiated from other members of the genus Nonomuraea on the basis of physiology and 16S rRNA gene sequence analysis. The 16S rRNA gene sequence similarity of strain DRQ-2(T) showed highest sequence similarity to Nonomuraea muscovyensis DSM 45913(T) (99.1%), N. salmonea DSM 43678(T) (98.2%) and N. maheshkhaliensis JCM 13929(T) with 98.0%, respectively. Chemotaxonomic properties showing predominant menaquinones of MK-9 (H-4), MK-9(H-2) and MK-9(H-6), major polar lipids comprised diphosphatidylglycerol, phosphatidylmono methyl ethanolamine (PME), phosphatidylethanolamine (PE), hydroxy-PME (OH-PME), hydroxy PE (OH-PEE), phosphatidylglycerol (PG), ninhydrin-positive phosphoglycolipid and unknown phospholipid, fatty acids with major amounts of i-C-16:0, ai-C-15:0 and ai-C-17:0 supported allocation of the strain to the genus Nonomuraea. Results of DNA-DNA hybridization and physiological tests allowed genotypic and phenotypic differentiation of strain DRQ-2(T) from closely related species. The genomic DNA G+C content of the organism was 72.5 mol%. On the basis of phenotypic, chemotypic and molecular characteristics, strain DRQ-2(T) represents a novel species of the genus Nonomuraea, for which the name N. indica sp. nov. is proposed, with type strain DRQ-2(T) (= NCIM 5480(T) = CCTCC AA 209050(T)).</p>1 aQuadri, Syed, Raziuddin1 aTian, Xin-Peng1 aZhang, Jing1 aLi, Jie1 aNie, Guo-Xing1 aTang, Shu-Kun1 aRuwaili, Jamal, Al1 aAgsar, Dayanand1 aLi, Wen-Jun1 aDastager, Syed, Gulam  uhttps://library.ncl.res.in/content/nonomuraea-indica-sp-nov-novel-actinomycetes-isolated-lime-stone-open-pit-mine-india-0