01595nas a2200205   4500008004100000245010600041210006900147260000800216300001200224490000700236520087100243100002201114700002501136700002501161700001701186700002401203700001901227700002501246856011801271       2017                        eng d00aSynthesis and cell imaging applications of fluorescent mono/di/tri-heterocycl- yl-2,6-dicyanoanilines0 aSynthesis and cell imaging applications of fluorescent monoditri  cDEC  a979-9880 v273 a<p>Synthesis of 3,4,5-triheterocyclyl-2,6-dicyanoanilines, starting from heterocyclic aldehydes and 1,2-diheterocycle-substituted ethanones, is described. 2,6-Dicyanoanilines with one or two heterocyclic substituents have also been synthesized. It was found that some of these molecules have selective cell-staining properties useful for cell imaging applications. The compounds 1g, 10f and 11 were found to stain cytoplasm of the cells in contact but not the nucleus while the compound 12 showed affinity to apoptotic cells resulting in blue fluorescence. The cell imaging results with compound 12 were similar to Annexin V-FITC, a known reagent containing recombinant Annexin V conjugated to green-fluorescent FITC dye, used for detection of apoptotic cells. These compounds were found to be non-cytotoxic and have potential application as cell imaging agents.</p>1 aPisal, Mahesh, M.1 aAnnadate, Ritesh, A.1 aAthalye, Meghana, C.1 aKumar, Dipak1 aChavan, Subhash, P.1 aSarkar, Dhiman1 aBorate, Hanumant, B.  uhttps://library.ncl.res.in/content/synthesis-and-cell-imaging-applications-fluorescent-monoditri-heterocycl-yl-26