01773nas a2200229 4500008004100000022001400041245014100055210006900196260007500265300001200340490000700352520086000359653002401219653004301243653001201286653003401298653002201332100001901354700002401373700002601397856012001423 2013 eng d a0006-292800aCloning and sequence analysis of three variants of the gene encoding alkaline xylanase C from the alkaliphilic bacillus sp (NCL 87-6-10)0 aCloning and sequence analysis of three variants of the gene enco a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGER/PLENUM PUBLISHERScOCT a737-7490 v513 a

Alkaline xylanase C from the alkaliphilic Bacillus sp. (NCL 87-6-10) has a low molecular weight and alkaline pI and is cellulase-free, properties compatible with its use in the prebleaching of pulp. We report here the cloning and sequence analysis of three variants of the gene encoding xylanase C; xyl C1, xyl C2, and xyl C3. In phylogenetic analysis, the three xylanase C variants clustered into a single group along with other reported alkaline xylanases. Residues contributing to the alkaline pH were present in all three variants. DNA and protein sequence comparison of these variants with other reported alkaline xylanases revealed silent mutations, some of which are due to codon preference in the respective organisms. The recombinant Xyl C1 that was successfully expressed in E. coli BL21 (DE3) had properties similar to the native enzyme.

10aAlkaline xylanase C10aAlkaliphilic Bacillus sp (NCL 87-6-10)10aCloning10aE. coli BL21 (DE3) expression10aSequence analysis1 aSharma, Poonam1 aRele, Meenakshi, V.1 aKumar, Lalitha, Sunil uhttps://library.ncl.res.in/content/cloning-and-sequence-analysis-three-variants-gene-encoding-alkaline-xylanase-c-002407nas a2200253 4500008004100000022001400041245016000055210006900215260007700284300001200361490000700373520141300380653002301793653001701816653001701833653003901850100002301889700002501912700002301937700002401960700002001984700001802004856013102022 2013 eng d a0038-905600aOptimization of the production of a maltooligosaccharides producing amylase from the alkalophilic streptomyces lonarensis strain NCL 716 using SVR modeling0 aOptimization of the production of a maltooligosaccharides produc aBOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANYbWILEY-V C H VERLAG GMBHcJAN a179-1850 v653 a

The Streptomyces lonarensis strain NCL 716 hydrolyses starch to produce a mixture of maltotriose (G3) and maltotetraose (G4) along with maltose (G2). The objective of the present work was to determine an optimum cost effective media composition for the production of a-amylase from this strain. The most influential factor was found to be starch while the least influential factor found was peptone by PlackettBurman method. Peptone amount was kept constant throughout the fermentation. Peptone, which is one of the expensive media components was used at a concentration of 1?g/L, which made the optimum media composition cost effective. A support vector regression-based process model was developed for approximating the non-linear relationship between the fermentation operating variables and the a-amylase yield. Multicanonical Jump Walk Annealing, a stochastic optimization technique is used to obtain optimal operating variables to maximize amylase yield. The maximum amylase activity thus obtained was in good agreement with the experimental values at the optimized levels. The optimum media composition obtained by this method was: yeast extract: 4.53?g/L, starch: 20.246?g/L, K2HPO4: 0.0827%, MgSO4: 0.15%, peptone: 1?g/L. A maximum enzyme activity of 297?U/mL, which was achieved using the above approaches compares well with the activity of reported amylases producing maltooligosaccharides.

10aAlkaline a-amylase10aOptimization10aStreptomyces10aSupport vector regression analysis1 aSharma, Trupti, K.1 aBhadane, Vaibhav, A.1 aKumar, Lalitha, S.1 aRele, Meenakshi, V.1 aBhawar, Gajanan1 aRahman, Imran uhttps://library.ncl.res.in/content/optimization-production-maltooligosaccharides-producing-amylase-alkalophilic-streptomyces-002731nas a2200289 4500008004100000022001400041245010000055210006900155260007400224300001200298490000800310520170400318653001602022653001702038653002402055653003602079100002302115700002502138700002402163700002302187700001502210700001602225700002402241700002602265700002602291856012402317 2016 eng d a0003-607200aStreptomyces lonarensis sp nov., isolated from lonar lake, a meteorite salt water lake in India0 aStreptomyces lonarensis sp nov isolated from lonar lake a meteor aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcFEB a225-2350 v1093 a

A novel alkaliphilic actinomycete, strain NCL716(T), was isolated from a soil sample collected from the vicinity of Lonar Lake, an alkaline salt water meteorite lake in Buldhana district of Maharashtra State in India. The strain was characterised using a polyphasic taxonomic approach which confirmed that it belongs to the genus Streptomyces. Growth was observed over a pH range of 7-11 at 28 A degrees C. The cell wall was found to contain ll-diaminopimelic acid and traces of meso-diaminopimelic acid. The major fatty acid components were identified as iso-C-16:0 (46.8 %), C-17:1 (12.4 %), anteiso-C-15:0 (5.1 %) and anteiso-C-17:1 (4.8 %). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The major menaquinones were determined to be MK-9 (H-6) (70.3 %), MK-9 (H-4) (15.5 %) and MK-9 (H-8) (7.2 %). The G+C content of the DNA of the type strain was determined to be 71.4 mol %. The 16S rRNA gene sequence has been deposited in GenBank with accession number FJ919811. Although the 16S rRNA gene sequence analysis revealed that strain NCL716(T) shares > 99 % similarity with that of Streptomyces bohaiensis strain 11A07(T), DNA-DNA hybridization revealed only 33.2 +/- A 3.0 % relatedness between them. Moreover, these two strains can be readily distinguished by some distinct phenotypic characteristics. Hence, on the basis of phenotypic and genetic analyses, it is proposed that strain NCL716(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces lonarensis sp. nov., is proposed. The type strain is NCL 716(T) (=DSM 42084(T) = MTCC 11708(T) = KCTC 39684(T)).

10aAlkaliphile10aDDH analysis10aPolyphasic taxonomy10aStreptomyces lonarensis sp nov.1 aSharma, Trupti, K.1 aMawlankar, Rahul, R.1 aSonalkar, Vidya, V.1 aShinde, Vidhya, K.1 aZhan, Jing1 aLi, Wen-Jun1 aRele, Meenakshi, V.1 aDastager, Syed, Gulam1 aKumar, Lalitha, Sunil uhttps://library.ncl.res.in/content/streptomyces-lonarensis-sp-nov-isolated-lonar-lake-meteorite-salt-water-lake-india-0