01733nas a2200277   4500008004100000022001400041245011000055210006900165260007900234300001200313490000800325520075200333653002201085653001701107653001501124653001101139653002401150653001301174100002501187700002201212700002701234700002401261700002401285700002101309856012501330       2011                        eng d  a0273-228900aIsolation, identification and optimization of a new extracellular lipase producing strain of rhizopus sp.0 aIsolation identification and optimization of a new extracellular  a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcAUG  a969-9780 v1643 a<p>A lipolytic mesophilic fungus which produces lipase extracellularly was isolated from soil. Based on ITS1-5.8S-ITS4 region sequences of ribosomal RNA, it was concluded that the isolate JK-1 belongs to genus Rhizopus and clades with Rhizopus oryzae. The present paper reports the screening, isolation, identification, and optimization of fermentation conditions for the production of lipase (EC 3.1.1.3). Culture conditions were optimized, and the highest lipase production was observed in basal medium with corn steep liquor as nitrogen source and glucose as carbon source. Maximum lipase production was observed at 72 h, which is about 870 U/ml. Optimization of fermentation conditions resulted in 16-fold enhancement in enzyme production.</p>10aCorn steep liquor10aFermentation10aITS region10aLipase10aMedium optimization10aRhizopus1 aKantak, Jayshree, B.1 aBagade, Aditi, V.1 aMahajan, Siddharth, A.1 aPawar, Shrikant, P.1 aShouche, Yogesh, S.1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/isolation-identification-and-optimization-new-extracellular-lipase-producing-strain-001799nas a2200229   4500008004100000022001400041245009000055210006900145260005700214300001200271490000700283520098800290100002401278700002401302700002001326700002101346700001601367700002401383700002601407700002101433856011501454       2013                        eng d  a0343-865100aAlishewanella solinquinati sp nov., isolated from soil contaminated with textile dyes0 aAlishewanella solinquinati sp nov isolated from soil contaminate  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcOCT  a454-4590 v673 a<p>A novel Gram-negative, motile, rod-shaped, facultative anaerobic bacterial strain, KMK6(T), was isolated from soil contaminated with textile dyes from an industrial estate located at Ichalkaranji, Maharashtra, India, and its taxonomical position was established by using a polyphasic approach. The major cellular fatty acids included C-17:1 omega 8c, summed feature 3 (C-16:1 omega 7c and/or iso-C-15:0 2-OH), C-17:0, C-16:0,C- and C-18:1 omega 7c. The DNA G+C content of strain KMK6(T) was 48.8 mol %. 16S rRNA gene sequence analysis confirmed its placement in the genus Alishewanella, and exhibited sequence similarity levels of below 97 % to the type strains of validly published Alishewanella species. On the basis of genotypic and phenotypic evidence, strains KMK6(T) is considered to be a novel species of the genus Alishewanella, for which we propose that strain KMK6(T) (=NCIM 5295(T) =BCRC 17848(T)) is assigned to a novel species, Alishewanella solinquinati sp. nov.</p>1 aKolekar, Yogesh, M.1 aPawar, Shrikant, P.1 aAdav, Sunil, S.1 aZheng, Liu-Qiang1 aLi, Wen-Jun1 aShouche, Yogesh, S.1 aDastager, Syed, Gulam1 aKodam, Kisan, M.  uhttps://library.ncl.res.in/content/alishewanella-solinquinati-sp-nov-isolated-soil-contaminated-textile-dyes-0