02147nas a2200253   4500008004100000022001400041245006700055210006400122260005700186300001200243490000800255520134200263653001701605653001801622653002001640653001501660100002601675700001301701700001901714700003001733700001801763700001601781856009601797       2014                        eng d  a0302-893300aArthrobacter enclensis sp. nov., isolated from sediment sample0 aArthrobacter enclensis sp nov isolated from sediment sample  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcNOV  a775-7820 v1963 a<p>A novel bacterial strain designated as NIO-1008(T) was isolated from marine sediments sample in Chorao Island India. Cells of the strains were gram positive and non-motile, displayed a rod-coccus life cycle and formed cream to light grey colonies on nutrient agar. Strain NIO-1008(T) had the chemotaxonomic markers that were consistent for classification in the genus Arthrobacter, i.e. MK-9(H-2) (50.3 %), as the major menaquinone, and the minor amount of MK-7 (H-2-27.5 %), MK-8 (H-4-11.6 %) and MK-8 (H-2-10.4 %). anteiso-C-15:0, iso-C-15:0, iso-C-16:0 and C-15:0 were the predominant fatty acids. Galactose, glucose and rhamnose are the cell-wall sugars, and DNA G+C content was 61.3 mol%. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that the strains were most similar to Arthrobacter equi IMMIB L-1606(T), Arthrobacter chlorophenolicus DSM 12829(T), Arthrobacter defluvii KCTC 19209(T) and Arthrobacter niigatensis CCTCC AB 206012(T) with 98.5, 98.4, 98.0 and 97.8 %, respectively, and formed a separate lineage. Combined phenotypic data and DNA-DNA hybridization data supported the conclusion that strains NIO-1008(T) represent a novel species within the genus Arthrobacter, for which the name Arthrobacter enclensis sp. nov., is proposed. The type strain is NIO-1008(T) = (NCIM 5488(T) = DSM 25279(T)).</p>10aArthrobacter10aChorao Island10aMarine sediment10aPolyphasic1 aDastager, Syed, Gulam1 aQin, Liu1 aTang, Shan-Kun1 aKrishnamurthi, Srinivasan1 aLee, Jae-Chan1 aLi, Wen-Jun  uhttps://library.ncl.res.in/content/arthrobacter-enclensis-sp-nov-isolated-sediment-sample-000721nas a2200193   4500008004100000022001400041245009100055210006900146260005700215300000800272490000800280100002600288700001400314700001900328700003000347700001800377700001600395856011600411       2014                        eng d  a0302-893300aArthrobacter enclensis sp. nov., isolated from sediment sample (vol 196, pg 775, 2014)0 aArthrobacter enclensis sp nov isolated from sediment sample vol   a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcNOV  a7830 v1961 aDastager, Syed, Gulam1 aLiu, Qing1 aTang, Shan-Kun1 aKrishnamurthi, Srinivasan1 aLee, Jae-Chan1 aLi, Wen-Jun  uhttps://library.ncl.res.in/content/arthrobacter-enclensis-sp-nov-isolated-sediment-sample-vol-196-pg-775-2014-002258nas a2200193   4500008004100000022001400041245011700055210006900172260011800241300001400359490000700373520143400380100002401814700002501838700003001863700001901893700002601912856012601938       2014                        eng d  a1466-502600aDomibacillus enclensis sp nov., isolated from marine sediment, and emended description of the genus domibacillus0 aDomibacillus enclensis sp nov isolated from marine sediment and   aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcDEC  a4098-41020 v643 a<p>A novel red-pigmented bacterial strain, designated NIO-1016(T), was isolated from a sediment sample from Chorao Island, India and was investigated by a polyphasic taxonomic approach. The strain was Gram-reaction-positive, strictly aerobic, motile and catalase-positive and produced spherical to slightly ellipsoidal endospores with subterminal position on swollen sporangia. The genomic DNA G+C content was 46.9 molok. The major fatty acids were anteiso-C-15:0, anteiso-C-17:0, iSO-C-15:0 and C-16:0. The predominant quinones were MK-6 (89.3 %) and MK-7 (8.7 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, and an unidentified phospholipid. meso-Diaminopimelic acid (type My) was present in the cell-wall peptidoglycan and the major whole-cell sugars were glucose and ribose. The closest phylogenetic neighbours were identified as Domibacillus robiginosus DSM 25058(T) (98.0% similarity) and Domibacillus indicus DSM 28032(T) (97.2% similarity), other species of the genus Bacillus displayed below 96% similarity. Phylogenetic, physiological, biochemical and morphological differences between strain NI0-1016(T) and its closest neighbours indicate that this strain represents a novel species in the genus Domibacillus in the family Bacillaceae for which the name Domibacillus enclensis sp. nov. is proposed with the type species N10-1016(T) (=DSM 25145(T)=NCIM 5462(T)=CCTCC AB 2011121(T)).</p>1 aSonalkar, Vidya, V.1 aMawlankar, Rahul, R.1 aKrishnamurthi, Srinivasan1 aTang, Shan-Kun1 aDastager, Syed, Gulam  uhttps://library.ncl.res.in/content/domibacillus-enclensis-sp-nov-isolated-marine-sediment-and-emended-description-genus-002516nas a2200217   4500008004100000022001400041245007500055210006900130260011800199300001400317490000700331520169700338100002602035700002502061700001902086700003002105700002002135700001802155700002402173856010102197       2014                        eng d  a1466-502600aRhodococcus enclensis sp nov., a novel member of the genus rhodococcus0 aRhodococcus enclensis sp nov a novel member of the genus rhodoco  aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcAUG  a2693-26990 v643 a<p>A novel actinobacterial strain, designated, NIO-1009(T), was isolated from a marine sediment sample collected from Chorao Island, Goa, India. Phylogenetic analysis comparisons based on 16S rRNA gene sequences between strain MO-1009(T) and other members of the genus Rhodococcus revealed that strain NI0-1009(T) had the closest sequence similarity to Rhodococcus kroppenstedtii DSM 44908(T) and Rhodococcus corynebacterioides DSM 20151(T) with 99.2 and 99.1%, respectively. Furthermore, DNA-DNA hybridization results showed that R. kroppenstedtii DSM 44908(T) and R. corynebacterioides DSM 20151(T) were 39.5 (3.0%) and 41.7 (2.0%) with strain NIO-1009(T), respectively, which were well below the 70% limit for any novel species proposal. Phylogenetically strain NIO-1009(T) forms a stable clade with and R. kroppenstedtii DSM 44908(T) and R. corynebacterioides DSM 201511 with 100% bootstrap values. Strain NI-1009(T) contained meso-diaminopimelic acid as the diagnostic diamino acid and galactose and arabinose as the cell wall sugars. The major fatty acids were C-16:0, C-18:1 omega 9c, C-16:1(omega 6C and/or omega 7c) and 10-methyl C-18:0. The only menaquinone detected was MK-8(H-2), while the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and one unknown phospholipid. The G + C content of the genomic DNA was 66.9 molok. The phenotypic and genotypic data showed that strain NIO-1009(T) warrants recognition as a novel species of the genus Rhodococcus for which the name Rhodococcus enclensis sp. nov., is proposed; the type strain is NIO-1009(T) (=NCIM 5452(T)=DSM 45688(T)).</p>1 aDastager, Syed, Gulam1 aMawlankar, Rahul, R.1 aTang, Shan-Kun1 aKrishnamurthi, Srinivasan1 aRamana, Venkata1 aJoseph, Neeta1 aShouche, Yogesh, S.  uhttps://library.ncl.res.in/content/rhodococcus-enclensis-sp-nov-novel-member-genus-rhodococcus-002272nas a2200217   4500008004100000022001400041245006100055210006100116260011800177300001400295490000700309520148700316100002601803700002501829700001701854700001801871700003001889700001801919700002401937856009301961       2015                        eng d  a1466-502600aBacillus encimensis sp nov isolated from marine sediment0 aBacillus encimensis sp nov isolated from marine sediment  aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcMAY  a1421-14250 v653 a<p>A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium designated SGD-V-25(T) was isolated from Veraval sediment sample, India. Strain SGD-V-25(T) was capable of growing at 25-50 degrees C (optimum 37 degrees C), pH 6-12 (optimum pH 7.0) and with 0-5 % (w/v) NaCl. The taxonomic position of this strain was deduced using a polyphasic approach and the 16S rRNA gene sequence analysis showed that the isolate belongs to the phylum Firmicutes, forming the cluster with Bacillus badius MTCC 1548(T), with which it shares highest similarity of 99.1 % with 13 nt differences. Other type strains of the genus Bacillus showed less than 96% similarity. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid. The polar lipid profile of strain SGD-V-25(T) showed the presence of diphosphatidylglycerol, phosphatidylglycerol, phsophoglycolipid and two aminophospholipids. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were iso-C-15:0, anteiso-C-15:0, anteiso-C-17:0 iso-C-16:0, C-16:1 omega 11c and C-16:0. The genomic DNA G + C content of strain SGD-V-25(T) was 37.6 molok. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA hybridization, strain SGD-V-25(T) could be clearly distinguished from closely related members of the genus Bacillus, and the name Bacillus encimensis sp. nov., is proposed to accommodate this strain. The type strain is SGD-V-25(T) (= NCIM 5513(T)=DSM 28241(T)).</p>1 aDastager, Syed, Gulam1 aMawlankar, Rahul, R.1 aMual, Poonam1 aVerma, Ashish1 aKrishnamurthi, Srinivasan1 aJoseph, Neeta1 aShouche, Yogesh, S.  uhttps://library.ncl.res.in/content/bacillus-encimensis-sp-nov-isolated-marine-sediment-002018nas a2200241   4500008004100000022001400041245006100055210005900116260011800175300001400293490000700307520117200314100002601486700002501512700002401537700002401561700001701585700001801602700003001620700001901650700001601669856009101685       2015                        eng d  a1466-502600aExiguobacterium enclense sp nov., isolated from sediment0 aExiguobacterium enclense sp nov isolated from sediment  aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcMAY  a1611-16160 v653 a<p>A Gram-stain-positive bacterium, designated strain NIO-1109(T), was isolated from a marine sediment sample from Chorao Island, Goa, India. Phenotypic and chemotaxonomic characteristics and data from phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NIO-1109(T) was related to the genus Exiguobacterium. Strain NIO-1109(T) exhibited &gt;98.0% 16S rRNA gene sequence similarity with respect to Exiguobacterium indicum HHS 31(T) (99.5%) and Exiguobacterium acetylicum NCIMB 9889(T) (99.1 %); the type strains of other species showed &lt;98% similarity. Levels of DNA DNA relatedness between strain NIO-1109(T) and E. acetylicum DSM 20416(T) and E indicum LMG 23471(T) were less than 70% (33.0 +/- 2.0 and 37 +/- 32%, respectively). Strain NIO-1109(T) also differed from these two closely related species in a number of phenotypic traits. Based on phenotypic, chemotaxonomic and phylogenetic data, strain NIO-1109(T) is considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium enclense sp. nov. is proposed. The type strain is NIO-1109(T) (=NCIM 5457(T)= DSM 25128(T)=CCTCC AB 2011124(T)).</p>1 aDastager, Syed, Gulam1 aMawlankar, Rahul, R.1 aSonalkar, Vidya, V.1 aThorat, Meghana, N.1 aMual, Poonam1 aVerma, Ashish1 aKrishnamurthi, Srinivasan1 aTang, Shan-Kun1 aLi, Wen-Jun  uhttps://library.ncl.res.in/content/exiguobacterium-enclense-sp-nov-isolated-sediment-002035nas a2200229   4500008004100000022001400041245006600055210006400121260005700185300001000242490000800252520127000260653001601530653001401546653002001560653002401580100002501604700002401629700003001653700002601683856009601709       2016                        eng d  a0302-893300aBacillus cellulasensis sp nov., isolated from marine sediment0 aBacillus cellulasensis sp nov isolated from marine sediment  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcJAN  a83-890 v1983 a<p>A novel bacterial strain NIO-1130(T) was isolated from sediment sample taken from Chorao Island, Goa Province, India, and subjected to a taxonomic investigation. The strain was Gram-positive, aerobic, and motile. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate within the genus Bacillus and strain NIO-1130(T) showed highest sequence similarity with Bacillus halosaccharovorans DSM 25387(T) (98.4 %) and Bacillus niabensis CIP 109816(T) (98.1 %), whereas other Bacillus species showed &lt; 97.0 % similarity. Tree based on gyrB gene sequence revealed that strain bacillus group. The major menaquinone was MK-7 and the predominant cellular fatty acids were iso-C-15:0, anteiso-C-15:0, iso-C-17:0, and anteiso-C-17:0. The strain showed a DNA G+C content of 39.9 mol%. DNA-DNA hybridization studies revealed that strain NIO-1130(T) exhibits 70 % similarity with Bacillus halosaccharovorans DSM 25387(T) and Bacillus niabensis CIP 109816T. On the basis of physiological, biochemical, chemotaxonomic and phylogenetic analyses, we consider the isolate to represent a novel species of the genus Bacillus, for which the name Bacillus cellulasensis sp. nov., is proposed. The type strain is NIO-1130(T) (=NCIM 5461(T) = CCTCC AB 2011126(T)).</p>10aBacillus sp10aCellulase10aMarine sediment10aPolyphasic taxonomy1 aMawlankar, Rahul, R.1 aThorat, Meghana, N.1 aKrishnamurthi, Srinivasan1 aDastager, Syed, Gulam  uhttps://library.ncl.res.in/content/bacillus-cellulasensis-sp-nov-isolated-marine-sediment-002044nas a2200217   4500008004100000022001400041245017300055210006900228260011800297300001400415490000700429520109900436100001701535700002401552700001801576700002001594700003001614700002601644700002401670856013201694       2016                        eng d  a1466-502600aReclassification of bacillus isronensis shivaji et al. 2009 as solibacillus isronensis comb. nov and emended description of genus solibacillus krishnamurthi et al. 20090 aReclassification of bacillus isronensis shivaji et al 2009 as so  aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcMAY  a2113-21200 v663 a<p>An investigation into the taxonomic position of Bacillus isronensis MTCC 7902(T) revealed that the strain shares a common phylogenetic lineage with Solibacillus silvestris MTCC 10789(T). It displays considerable overlap in phenotypic properties with the genus Solibacillus, including endospore shape and position, oxidase and catalase activities, presence of iso-C-15 : 0, C-16 : (1)omega 7c alcohol and iso-C-17 : 1 omega 7c as major cellular fatty acids, A4 alpha-type cell-wall peptidoglycan, polar lipids and menaquinone pattern. These features reinforce the findings of molecular phylogenetic analyses based on 16S rRNA gene, gyrB gene and 16S-23S internal transcribed spacer (ITS) region sequences and, in line with the recommendations of Kampfer et al. [Int J Syst Evol Microbiol 56 (2006), 781-786], provide justification for the transfer of Bacillus isronensis from the genus Bacillus to Solibacillus as Solibacillus isronensis comb. nov. The type strain is B3W22(T) (=MTCC 7902(T) =DSM 21046(T) =JCM 13838(T)). An emended description of the genus Solibacillus is also provided.</p>1 aMual, Poonam1 aSingh, Nitin, Kumar1 aVerma, Ashish1 aSchumann, Peter1 aKrishnamurthi, Srinivasan1 aDastager, Syed, Gulam1 aMayilraj, Shanmugam  uhttps://library.ncl.res.in/content/reclassification-bacillus-isronensis-shivaji-et-al-2009-solibacillus-isronensis-comb-nov-and02073nas a2200193   4500008004100000245013600041210006900177260000800246300001400254490000700268520133100275100001801606700002201624700002301646700002701669700002401696700003001720856012901750       2017                        eng d00aDomibacillus mangrovi sp nov and domibacillus epiphyticus sp nov., isolated from marine habitats of the central west coast of India0 aDomibacillus mangrovi sp nov and domibacillus epiphyticus sp nov  cAUG  a3063-30700 v673 aWhile studying culturable bacterial diversity in different marine habitats of the central west coast of India, two novel Gramstain-positive, strictly aerobic, motile, endospore-forming and rod-shaped bacterial strains designated as SAOS 44(T) and SAB 38(T) were isolated from mangrove sediment soil and the surface of a macroalga, respectively. The strains were taxonomically characterized by using a polyphasic approach and genomic methods. The phylogenetic analysis based on 16S rRNA gene sequencing placed the strains firmly in the genus Domibacillus and were most closely related to Domibacillus antri KCTC 33636(T). The predominant fatty acids were iso-C-15 : 0 and anteiso-C-15 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol in addition to an aminophosphoglycolipid. MK-6 was the only respiratory quinone. The range of values of digital DNA-DNA hybridization (19.2-24.9 %) and the ortho-average nucleotide identity (74.1-81.4 %) among strains SAOS 44(T), SAB 38(T) and other Domibacillus species clearly supports their status as a distinct and novel species for which the names Domibacillus mangrovi sp. nov. SAOS 44(T) (=DSM 100930(T)=KCTC 33820(T)=MTCC 12571(T)) and Domibacillus epiphyticus sp. nov. SAB 38(T) (=DSM 100929(T)= KCTC 33830(T)=MTCC 12575(T)) are proposed, respectively.1 aVerma, Ashish1 aOjha, Anup, Kumar1 aDastager, Syed, G.1 aNatarajan, Rameshkumar1 aMayilraj, Shanmugam1 aKrishnamurthi, Srinivasan  uhttps://library.ncl.res.in/content/domibacillus-mangrovi-sp-nov-and-domibacillus-epiphyticus-sp-nov-isolated-marine-habitats02384nas a2200217   4500008004100000245026000041210006900301260000800370520143900378100001801817700001401835700001701849700002201866700002701888700002001915700002601935700002801961700002401989700003002013856012302043       2017                        eng d00aExamination into the taxonomic position of Bacillus thermotolerans Yang et al., 2013, proposal for its reclassification into a new genus and species Quasibacillus thermotolerans gen. nov., comb. nov. and reclassification of B. encimensis Dastager et al., 0 aExamination into the taxonomic position of Bacillus thermotolera  cSEP3 aTwo novel Gram-staining positive, rod-shaped, moderately halotolerant, endospore forming bacterial strains 5.5LF 38TD and 5.5LF 48TD were isolated and taxonomically characterized from a landfill in Chandigarh, India. The analysis of 16S rRNA gene sequences of the strains confirmed their closest identity to Bacillus thermotolerans SgZ-8T with 99.9% sequence similarity. A comparative phylogenetic analysis of strains 5.5LF 38TD, 5.5LF 48TD and B. thermotolerans SgZ-8(T) confirmed their separation into a novel genus with B. badius and genus Domibacillus as the closest phylogenetic relatives. The major fatty acids of the strains are iso-C15:0 and iso-C16:0 and MK-7 is the only quinone. The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The digital DNA-DNA hybridization (DDH) and ortho average nucleotide identity (ANI) values calculated through whole genome sequences indicated that the three strains showed low relatedness with their phylogenetic neighbours. Based on evidences from phylogenomic analyses and polyphasic taxonomic characterization we propose reclassification of the species B. thermotolerans into a novel genus named Quasibacillus thermotolerans gen. nov., comb. nov with the type strain SgZ-8(T) (=CCTCC AB2012108(T)=KACC 16706(T)). Further our analyses also revealed that B. encimensis SGD-V-25(T) is a later heterotypic synonym of Bacillus badius DSM 23(T).1 aVerma, Ashish1 aPal, Yash1 aKhatri, Indu1 aOjha, Anup, Kumar1 aGruber-Vodicka, Harald1 aSchumann, Peter1 aDastager, Syed, Gulam1 aSubramanian, Srikrishna1 aMayilraj, Shanmugam1 aKrishnamurthi, Srinivasan  uhttps://library.ncl.res.in/content/examination-taxonomic-position-bacillus-thermotolerans-yang-et-al-2013-proposal-its02587nas a2200301   4500008004100000022001400041245014900055210006900204260000800273300001200281490000800293520153000301653001301831653002901844653001401873653002901887100001801916700002201934700001401956700002001970700002001990700002702010700002602037700002902063700002402092700003002116856013902146       2017                        eng d  a0723-202000aInvestigation into the taxonomy of “ B. aminovorans” and its reclassification to the genus Domibacillus as Domibacillus aminovorans sp. nov.0 aInvestigation into the taxonomy of B aminovorans and its reclass  cAUG  a458-4670 v40 3 aThe species "Bacillus aminovorans" was categorized as species Incertae Sedis meaning taxa with uncertain taxonomic position because of insufficient description of phenotypic properties and lack of understanding of its phylogenetic relationship (Claus and Berkeley [5]). In this study two strains "B. aminovorans" DSM 1314T and DSM 4337 were phenotypically and phylogenomically analyzed. The strains are Gram-staining-positive, spore forming rods and unable to utilize/ferment most of the sugars tested except glucose. The major fatty acids are anteiso-C15:0 and iso-C15:0. The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol and an aminophosphoglycolipid. The cell wall peptidoglycan is of A1γ type with meso-Dpm as the diamino acid. The menaquinone type present is MK-6. The G+C content of the genomic DNA of the type strain is 40.8mol%. These characteristics were found to be in line with description of the genus Domibacillus. Moreover phylogenetic analysis based on 16S rRNA gene sequence retrieved "B. aminovorans" within the genus Domibacillus with D. antri XD 80T as the closest relative. Additionally genome sequencing of the strains and their comparison with whole genome sequences of other Domibacillus spp. confirmed their distinctiveness and separate species status within the genus based on parameters of genome to genome distance calculator (GGDC) and average nucleotide identity (ANI) values. Therefore a novel species Domibacillus aminovorans sp. nov. (DSM 1314T =LMG 16796T) is proposed.10a16S rRNA10aDomibacillus aminovorans10aMALDI-TOF10a``Bacillus aminovorans''1 aVerma, Ashish1 aOjha, Anup, Kumar1 aPal, Yash1 aKumari, Parveen1 aSchumann, Peter1 aGruber-Vodicka, Harald1 aDastager, Syed, Gulam1 aNatarajan, Ramesh, Kumar1 aMayilraj, Shanmugam1 aKrishnamurthi, Srinivasan  uhttps://library.ncl.res.in/content/investigation-taxonomy-%E2%80%9C-b-aminovorans%E2%80%9D-and-its-reclassification-genus-domibacillus02972nas a2200337   4500008004100000022001400041245022400055210006900279260000800348300001200356490000700368520179200375653001302167653001302180653003002193653001602223653001702239653001902256100001802275700001402293700002202307700001902329700001702348700002002365700002002385700002302405700002402428700002802452700003002480856012402510       2019                        eng d  a0723-202000aTaxonomic insights into the phylogeny of Bacillus badius and proposal for its reclassification to the genus Pseudobacillus as Pseudobacillus badius comb. nov. and reclassification of Bacillus wudalianchiensis Liu et al.0 aTaxonomic insights into the phylogeny of Bacillus badius and pro  cMAY  a360-3720 v423 a<p>The species Bacillus badius is one of the oldest members of the genus Bacillus isolated from faeces of children and was classified based on its ability to form endospores [8]. In 16S rRNA gene sequence and phylogenetic analysis, Bacillus badius DSM 23(T) shared low similarity (93.0%) and distant relationship with B. subtilis, the type species of the genus Bacillus indicating that it does not belong to this genus. Additional strains of the species, B. badius DSM 5610, DSM 30822 and B. encimensis SGD-V-25 (which has been recently reclassified as a member of this species) were included in the study to consider intraspecies diversity. Detailed molecular phylogenetic and comparative genome analysis clearly showed that the strains of B. badius were consistently retrieved outside the cluster of Bacillus sensu stricto and also distantly related to the genera Domibacillus and Quasibacillus. Further, the data from biochemical reactions (inability to ferment most carbohydrates), polar lipids profile (presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an aminophosphoglycolipid) and fatty acids supported the molecular analysis. Thus the four B. badius strains; DSM 23(T), DSM 5610, DSM 30822 and SGD-V-25 displayed sufficient demarcating phenotypic characteristics that warrant their classification as members of a novel genus and single species, for which the name Pseudobacillus badius gen. nov. comb. nov. is proposed with Pseudobacillus badius DSM 23(T) (= ATCC 14574(T)) as the type strain. Additionally, based on our findings from phenotypic, chemotaxonomic and genotypic parameters, Bacillus wudalianchiensis DSM 100757(T) was reclassified as Pseudobacillus wudalianchiensis comb. nov. (C) 2019 Elsevier GmbH. All rights reserved.</p>
10a16S rRNA10aBacillus10aBacillus wudalianchiensis10aFatty acids10apolar lipids10aPseudobacillus1 aVerma, Ashish1 aPal, Yash1 aOjha, Anup, Kumar1 aKumari, Munesh1 aKhatri, Indu1 aRameshkumar, N.1 aSchumann, Peter1 aDastager, Syed, G.1 aMayilraj, Shanmugam1 aSubramanian, Srikrishna1 aKrishnamurthi, Srinivasan  uhttps://library.ncl.res.in/content/taxonomic-insights-phylogeny-bacillus-badius-and-proposal-its-reclassification-genus07622nas a2201957   4500008004100000022001400041245016800055210006900223260000800292300001100300490000800311520199500319653001102314653001202325653001302337653002102350653001402371653002102385653001502406100001902421700002102440700002302461700001902484700001902503700002002522700002402542700001902566700001802585700002002603700001502623700002702638700003402665700002102699700001702720700001702737700001902754700002402773700003002797700001902827700001802846700002102864700001402885700002502899700001702924700001702941700001202958700002002970700001902990700001703009700002103026700002603047700002603073700001903099700001803118700002403136700003003160700002703190700001703217700002703234700003503261700002503296700002403321700002303345700002803368700002703396700002703423700002103450700002903471700001903500700001603519700001703535700001803552700001603570700001803586700001703604700002003621700001903641700002403660700002103684700001903705700001803724700002203742700002203764700002403786700002603810700002203836700002403858700002803882700002103910700001803931700001703949700002603966700001603992700001704008700001904025700002204044700001804066700001604084700001904100700002704119700002704146700002404173700001704197700002104214700002304235700001804258700002504276700002804301700001904329700002204348700002204370700001604392700001804408700002004426700001704446700001904463700001504482700002704497700003004524700001604554700001204570700001804582700002804600700002204628700002104650700002704671700002004698700001804718700002104736700001804757700002004775700002004795700001704815700001904832700001604851700001904867700001604886700002004902700002204922700002104944700001804965700001904983700002105002700002005023700002005043700002105063700002005084700002205104700003105126700001905157700002705176700003505203700002605238700002405264700002405288700003205312700003905344700002305383700001805406700002405424700002505448700002005473700002305493700001805516856013005534       2022                        eng d  a0010-482500aMachine learning-based approach to determine infection status in recipients of BBV152 (Covaxin) whole-virion inactivated SARS-CoV-2 vaccine for serological surveys0 aMachine learningbased approach to determine infection status in   cJUL  a1054190 v1463 a<p>Data science has been an invaluable part of the COVID-19 pandemic response with multiple applications, ranging from tracking viral evolution to understanding the vaccine effectiveness. Asymptomatic breakthrough infections have been a major problem in assessing vaccine effectiveness in populations globally. Serological discrimination of vaccine response from infection has so far been limited to Spike protein vaccines since whole virion vaccines generate antibodies against all the viral proteins. Here, we show how a statistical and machine learning (ML) based approach can be used to discriminate between SARS-CoV-2 infection and immune response to an inactivated whole virion vaccine (BBV152, Covaxin). For this, we assessed serial data on antibodies against Spike and Nucleocapsid antigens, along with age, sex, number of doses taken, and days since last dose, for 1823 Covaxin recipients. An ensemble ML model, incorporating a consensus clustering approach alongside the support vector machine model, was built on 1063 samples where reliable qualifying data existed, and then applied to the entire dataset. Of 1448 self-reported negative subjects, our ensemble ML model classified 724 to be infected. For method validation, we determined the relative ability of a random subset of samples to neutralize Delta versus wild-type strain using a surrogate neutralization assay. We worked on the premise that antibodies generated by a whole virion vaccine would neutralize wild type more efficiently than delta strain. In 100 of 156 samples, where ML prediction differed from self-reported uninfected status, neutralization against Delta strain was more effective, indicating infection. We found 71.8% subjects predicted to be infected during the surge, which is concordant with the percentage of sequences classified as Delta (75.6%-80.2%) over the same period. Our approach will help in real-world vaccine effectiveness assessments where whole virion vaccines are commonly used.</p>
10aBBV15210aCovaxin10aCOVID-1910aEnsemble methods10aInfection10amachine learning10aSARS-CoV-21 aSingh, Prateek1 aUjjainiya, Rajat1 aPrakash, Satyartha1 aNaushin, Salwa1 aSardana, Viren1 aBhatheja, Nitin1 aSingh, Ajay, Pratap1 aBarman, Joydeb1 aKumar, Kartik1 aGayali, Saurabh1 aKhan, Raju1 aRawat, Birendra, Singh1 aTallapaka, Karthik, Bharadwaj1 aAnumalla, Mahesh1 aLahiri, Amit1 aKar, Susanta1 aBhosale, Vivek1 aSrivastava, Mrigank1 aMugale, Madhav, Nilakanth1 aPandey, C., P.1 aKhan, Shaziya1 aKatiyar, Shivani1 aRaj, Desh1 aIshteyaque, Sharmeen1 aKhanka, Sonu1 aRani, Ankita1 aPromila1 aSharma, Jyotsna1 aSeth, Anuradha1 aDutta, Mukul1 aSaurabh, Nishant1 aVeerapandian, Murugan1 aVenkatachalam, Ganesh1 aBansal, Deepak1 aGupta, Dinesh1 aHalami, Prakash, M.1 aPeddha, Muthukumar, Serva1 aVeeranna, Ravindra, P.1 aPal, Anirban1 aSingh, Ranvijay, Kumar1 aAnandasadagopan, Suresh, Kumar1 aKaruppanan, Parimala1 aRahman, Syed, Nasar1 aSelvakumar, Gopika1 aVenkatesan, Subramanian1 aKarmakar, Malay, Kumar1 aSardana, Harish, Kumar1 aKothari, Anamika1 aParihar, Devendra, Singh1 aThakur, Anupma1 aSaifi, Anas1 aGupta, Naman1 aSingh, Yogita1 aReddu, Ritu1 aGautam, Rizul1 aMishra, Anuj1 aMishra, Avinash1 aGogeri, Iranna1 aRayasam, Geethavani1 aPadwad, Yogendra1 aPatial, Vikram1 aHallan, Vipin1 aSingh, Damanpreet1 aTirpude, Narendra1 aChakrabarti, Partha1 aMaity, Sujay, Krishna1 aGanguly, Dipyaman1 aSistla, Ramakrishna1 aBalthu, Narender, Kumar1 aKumar, Kiran, A.1 aRanjith, Siva1 aKumar, Vijay1 aJamwal, Piyush, Singh1 aWali, Anshu1 aAhmed, Sajad1 aChouhan, Rekha1 aGandhi, Sumit, G.1 aSharma, Nancy1 aRai, Garima1 aIrshad, Faisal1 aJamwal, Vijay, Lakshmi1 aPaddar, Masroor, Ahmad1 aKhan, Sameer, Ullah1 aMalik, Fayaz1 aGhosh, Debashish1 aThakkar, Ghanshyam1 aBarik, S., K.1 aTripathi, Prabhanshu1 aSatija, Yatendra, Kumar1 aMohanty, Sneha1 aKhan, Md, Tauseef1 aSubudhi, Umakanta1 aSen, Pradip1 aKumar, Rashmi1 aBhardwaj, Anshu1 aGupta, Pawan1 aSharma, Deepak1 aTuli, Amit1 aChaudhuri, Saumya, Ray1 aKrishnamurthi, Srinivasan1 aPrakash, L.1 aRao, Ch1 aSingh, B., N.1 aChaurasiya, Arvindkumar1 aChaurasiya, Meera1 aBhadange, Mayuri1 aLikhitkar, Bhagyashree1 aMohite, Sharada1 aPatil, Yogita1 aKulkarni, Mahesh1 aJoshi, Rakesh1 aPandya, Vaibhav1 aMahajan, Sachin1 aPatil, Amita1 aSamson, Rachel1 aVare, Tejas1 aDharne, Mahesh1 aGiri, Ashok1 aMahajan, Sachin1 aParanjape, Shilpa1 aSastry, Narahari1 aKalita, Jatin1 aPhukan, Tridip1 aManna, Prasenjit1 aRomi, Wahengbam1 aBharali, Pankaj1 aOzah, Dibyajyoti1 aSahu, RaviKumar1 aDutta, Prachurjya1 aSingh, Moirangthem, Goutam1 aGogoi, Gayatri1 aTapadar, Yasmin, Begam1 aBabu, Elapavalooru, V. S. S. K1 aSukumaran, Rajeev, K.1 aNair, Aishwarya, R.1 aPuthiyamadam, Anoop1 aValappil, Prajeesh, Kooloth1 aPrasannakumari, Adrash, Velayudhan1 aChodankar, Kalpana1 aDamare, Samir1 aAgrawal, Ved, Varun1 aChaudhary, Kumardeep1 aAgrawal, Anurag1 aSengupta, Shantanu1 aDash, Debasis  uhttps://library.ncl.res.in/content/machine-learning-based-approach-determine-infection-status-recipients-bbv152-covaxin-whole