02001nas a2200193   4500008004100000245014500041210006900186260000800255300001200263490000900275520127500284100001801559700002001577700002001597700002201617700001801639700002201657856012801679       2017                        eng d00aStability studies of cuticle degrading and mycolytic enzymes of myrothecium verrucaria for control of insect pests and fungal phytopathogens0 aStability studies of cuticle degrading and mycolytic enzymes of   cJUN  a404-4120 v16  3 aMyrothecium verrucaria produced extracellularly hydrolytic enzymes which can hydrolyse the insect cuticle as well as fungal cell wall. The addition of polyols, such as glycerol, sorbitol, xylitol (1 M) during ultra -filtration or freeze- drying of enzyme mixture increased the recovery of the enzymes in a concentrated form. Polyols (5 M) increased the temperature and pH stability of the enzymes, in the presence of glycerol chitinase, beta-1,3-glucanase, lipase and protease retained 50-60% of initial activities at 40 degrees C after 3 h. While xylitol (5 M) was effective in stabilizing activities at pH 5.0 and 7.5 at 25 degrees C for 7 d. In the freeze-dried powder form, > 90% at 4 degrees C for 1 year and 80-85% at 25 degrees C for 2 months enzyme activities were retained. The addition of glycerol (1 M) to the enzyme mixture protected enzyme activities under sunlight (60-65% activity at RT) for 5 d. Because of glycerol (1 M), the efficacy of M verrucaria enzyme preparation to control Helicoverpa armigera infestation in chick pea was increased to 70 +/- 19%. While without glycerol the efficacy was 55 +/- 23%. The germination of peanut seeds infected with Sclerotium rolfsii was observed to be increased (70 +/- 5%) in a pot irrigated with enzyme mixture.1 aChavan, S., B1 aVidhate, R., P.1 aKallure, G., S.1 aDandawate, N., L.1 aKhire, J., M.1 aDeshpande, M., V.  uhttps://library.ncl.res.in/content/stability-studies-cuticle-degrading-and-mycolytic-enzymes-myrothecium-verrucaria-control01830nas a2200205   4500008004100000022001500041245012200056210006900178260000800247300002600255490000700281520108200288100002301370700002001393700002101413700001901434700002101453700002201474856012801496       2018                        eng d  a 1567-135600aDetermination of the effect of polyamines on an oil-degrading strain of Yarrowia lipolytica using an odc minus mutant0 aDetermination of the effect of polyamines on an oildegrading str  cDEC  a10.1093/femsyr/foy0730 v183 aYarrowia lipolytica is an ascomycetous dimorphic yeast with immense potential for industrial applications, including bioremediation of crude oil-contaminated environments. It has been shown that a dimorphic marine isolate of Y. lipolytica (var. indica) has significant capacity to degrade fatty acids and alkanes, when in its yeast morphology. It has also been demonstrated that polyamines play an important role in the yeast-to-mycelium transition of different strains of Y. lipolytica that are unable to utilize those carbon sources. To determine the role of polyamines on their capacity to utilize oils and hydrocarbons, on the dimorphic transition, and also on other characteristics of the var. indica strain of Y. lipolytica, we proceeded to obtain ornithine decarboxylase minus (odc(-)) mutants. These mutants behaved as yeasts independently of the concentrations of putrescine added. Further, they conserved the oil-degrading capacity of the parent strain. The odc(-) mutant can thus be used in fatty acid degradation, and oil spill remediation with distinct advantages.1 aCampos-Gongora, E.1 aPalande, A., S.1 aLeon-Ramirez, C.1 aPathan, E., K.1 aRuiz-Herrera, J.1 aDeshpande, M., V.  uhttps://library.ncl.res.in/content/determination-effect-polyamines-oil-degrading-strain-yarrowia-lipolytica-using-odc-minus01532nas a2200145   4500008004100000245005600041210005400097260001300151300001200164520106400176100001901240700001801259700002201277856008701299       2019                        eng d00aBioprospecting of fungal entomo- and myco-pathogens0 aBioprospecting of fungal entomo and mycopathogens  bSpringer  a497-5133 aThe pest and pathogen control in the field using fungi and their metabolites has indeed gone beyond ‘proof of concept’. However, in view of the performances of these biocontrol fungi in the fields, moreover, acceptability by the end users regarding cost-effectiveness, shelf life, intellectual property rights (IPR), the additional roles, and possible applications are being explored. Dual pathogenicity, viz. entomopathogenesis and mycoparasitism, has added the advantage of wide-spectrum biocontrol in single-crop system. In addition to plant protection, these fungi can promote plant growth. Further, in integrated pest management, the bioremediation of pesticide residues, if any, can also be achieved by these organisms. For value addition, use of cuticle-degrading and mycolytic enzymes produced as killing components can be explored in food, detergent, organic synthesis, and pharmaceutical industries. While exploring the additional applications, the safety to beneficial insects and fungi, biodiversity, and humans, in general, is also addressed.1 aPathan, E., K.1 aPatil, A., V.1 aDeshpande, M., V.  uhttps://library.ncl.res.in/content/bioprospecting-fungal-entomo-and-myco-pathogens