01669nas a2200193 4500008004100000245011600041210006900157260000800226520091800234100002101152700002701173700002701200700002201227700001901249700003001268700002701298700002901325856012101354 2017 eng d00aStudy of nanofiber scaffolds of PAA, PAA/CS, and PAA/ALG for its potential use in biotechnological applications0 aStudy of nanofiber scaffolds of PAA PAACS and PAAALG for its pot cMAY3 aIn recent times, electrospun nanofibers have been widely studied from several biotechnological approaches; in this work, poly (acrylic acid) solutions mixed with chitosan and alginate were electrospun and characterized in order to determine the behavior of these fibers when used in combination with bacteria, different samples were incubated with the bacterial strains: Streptomyces spp., Micromonospora spp. and Escherichia coli and a OD600 test was performed. The formation of nanofibers via electrospinning and the physicochemical properties of the obtained fibers were evaluated. Results showed that the presence of Chitosan enhanced the thermal stability of poly (acrylic acid), since poly (acrylic acid)/alginate fibers lost 5% of their mass at 41°C while poly (acrylic acid)/chitosan lost this amount at around 125°C. The fibers demonstrated suitable characteristics to be used as a bacteria bioreactor.1 aRodolfo, Velasco1 aRicardo, Vera-Graziano1 aLópez-Maldonado, E.A.1 aMercedes, Oropeza1 aDastager, Syed1 aAdriana, Álvarez-Andrade1 aAna, leticia, Iglesias1 aLuis, Jesús, Villarreal uhttps://library.ncl.res.in/content/study-nanofiber-scaffolds-paa-paacs-and-paaalg-its-potential-use-biotechnological02137nas a2200193 4500008004100000245013500041210006900176260000800245490000700253520142000260100002501680700001701705700001801722700001901740700001601759700001801775700001901793856013101812 2018 eng d00aEvaluation of Candida tropicalis (NCIM 3321) extracellular phytase having plant growth promoting potential and process development0 aEvaluation of Candida tropicalis NCIM 3321 extracellular phytase cJAN0 v133 a

Phytase is known to provide a solution for depletion of phosphorus (P). It helps it by hydrolyzing the insoluble P source in soil which is phytate. In this study, provides insight on yeast Candida tropicalis (NCIM 3321) which produces cell bound and extracellular thermostable phytase. The media components were optimized to enhance the enzyme production and checked for plant growth promoting activity. On optimization the isolate exhibited enhanced cell bound and extracellular phytase activity by four folds (from 236 to 1024 IU DCG⁻¹) and by five folds (from 0.46 to 1.95 IU ml⁻¹) respectively in 36 h. The production time decreased to 24 h compare to shake flask on Up-scaling the production process upto 10 L scale, thus increasing the productivity of cell bound (1810 IU DCG⁻¹day⁻¹) and extracellular phytase (6.08 IU ml⁻¹ day⁻¹). The crude phytase (12 IU) from NCIM 3321 strain was studied for plant growth promotion activity in lab scale and field level experiments with maize crop. Findings of the study revealed that the extracellular phytase derived from non pathogenic C. tropicalis (NCIM 3321) was found to be plant growth stimulating by increasing the available P in soil. Our findings of phytase isolated from non-pathogenic yeast C. tropicalis NCIM 3321 exhibited dephytinization potential. Therefore, current study may have profound application in sustainable agriculture.

1 aPuppala, Kumar, Raja1 aNaik, Tejali1 aShaik, Alfina1 aDastager, Syed1 aKumar, Ravi1 aKhire, Jayant1 aDharne, Mahesh uhttps://library.ncl.res.in/content/evaluation-candida-tropicalis-ncim-3321-extracellular-phytase-having-plant-growth-promoting02399nas a2200145 4500008004100000245013100041210006900172260000800241520180000249100002002049700002002069700001902089700002202108856012302130 2019 eng d00aDevelopment of low-cost plant probiotic formulations of functional endophytes for sustainable cultivation of Coleus forskohlii0 aDevelopment of lowcost plant probiotic formulations of functiona cAUG3 a

Deployment of plant endophytes at field level is reported to make an impact on agricultural crop productivity; development and deployment of suitable crop specific plant probiotics in a suitable delivery matrix is a value-added task. In our study, we attempted to develop bioformulations of native, fungal endophytes of Coleus forskohlii to improve plant yield using two different carrier-based materials (talc and wheat bran). Initially, fungal endophytes (RF1, SF1, and SF2) were grown on sterilized wheat bran under solid state condition and their growth kinetics and pattern were analyzed by ergosterol content and scanning electron microscope, respectively. 10-day-grown fungal endophytic cultures were used for the development of two types of formulations (wheat bran and talc-based formulations) and tested for their efficacy on host plant, C. forskohlii under field conditions. Interestingly, application of wheat bran-based endophytic formulations significantly (p < 0.01) enhanced plant height (12-29%), number of branches (51-63%), root biomass (26-33%), photosynthetic pigments (32-101%), and forskolin content (35-56%) compared to talc-based formulations under field conditions. Shelf life of endophytes (RF1, SF1, and SF2) in both formulations revealed spore viability in wheat bran-based formulations for 6 months storage period as compared to talc-based formulations. Overall, the present investigation envisages developing plant probiotic bioformulations of functional endophytes of C. forskohlii to enhance root biomass and in planta forskolin content.

1 aMastan, Anthati1 aRane, Digeshwar1 aDastager, Syed1 aVivekBabu, C., S. uhttps://library.ncl.res.in/content/development-low-cost-plant-probiotic-formulations-functional-endophytes-sustainable01129nas a2200229 4500008004100000245012400041210006900165260000800234520028600242100001500528700002500543700001600568700002700584700002200611700001900633700001900652700002300671700001800694700003100712700002400743856013200767 2019 eng d00aDraft genome sequence of freshwater-derived streptomyces sp. strain BPSDS2, isolated from damte stream, northeast India0 aDraft genome sequence of freshwaterderived streptomyces sp strai cOCT3 a

We report the draft genome sequence of Streptomyces sp. strain BPSDS2, isolated from freshwater sediments in Northeast India. The draft genome has a size of 8.27 Mb and 7,559 protein-coding sequences.

1 aZothanpuia1 aPassari, Ajit, Kumar1 aDeka, Vinay1 aRajput, Lakshmi, P. M.1 aPriya, Purbajyoti1 aDharne, Mahesh1 aDastager, Syed1 aMathew, Oommen, K.1 aHashem, Abeer1 aAllah, Elsayed, Fathi Abd_1 aSingh, Bhim, Pratap uhttps://library.ncl.res.in/content/draft-genome-sequence-freshwater-derived-streptomyces-sp-strain-bpsds2-isolated-damte-stream01942nas a2200229 4500008004100000022001400041245008000055210006900135260000800204490000700212520122900219653001501448653001301463653001801476653001501494653003201509100001801541700001901559700001901578700001901597856009601616 2021 eng d a2190-572X00aProbiotics in the prophylaxis of COVID-19: something is better than nothing0 aProbiotics in the prophylaxis of COVID19 something is better tha cJAN0 v113 a

The new viral pandemic of COVID-19 is caused by a novel coronavirus (SARS-CoV-2) that has brought the world at another unprecedented crisis in terms of health and economy. The lack of specific therapeutics necessitates other strategies to prevent the spread of infection caused by this previously unknown viral etiological agent. Recent pieces of evidence have shown an association between COVID-19 disease and intestinal dysbiosis. Probiotics comprise living microbes that upon oral administration benefit human health by reshaping the composition of gut microbiota. The close kinship of the gastrointestinal and respiratory tract suggests why the dysfunction of one may incite illness in others. The emerging studies suggest the capability of probiotics to regulate immune responses in the respiratory system. The efficacy of probiotics has been studied previously on several respiratory tract viral infections. Therefore, the purpose of this review is to comprehend existing information on the gut mediated-pulmonary immunity conferred by probiotic bacteria, in the course of respiratory virus infections and administration as a prophylactic measure in COVID-19 pandemic in managing intestinal dysbiosis as well.

10aAnti-viral10aCOVID-1910aGut-lung axis10aProbiotics10aRespiratory tract infection1 aGohil, Kushal1 aSamson, Rachel1 aDastager, Syed1 aDharne, Mahesh uhttps://library.ncl.res.in/content/probiotics-prophylaxis-covid-19-something-better-nothing02228nas a2200241 4500008004100000022001400041245011300055210006900168260000800237300001600245490000700261520141800268653001701686653001801703653001501721653001601736653001501752100002601767700002301793700001901816700001901835856013201854 2021 eng d a0944-134400aTranslating SARS-CoV-2 wastewater-based epidemiology for prioritizing mass vaccination: a strategic overview0 aTranslating SARSCoV2 wastewaterbased epidemiology for prioritizi cAUG a42975-429800 v283 aThe inception of the novel coronavirus has forced the world into despair. Rapid progress has been made in addressing the situation, and various clinical diagnostic methods were developed for early detection of transmission. However, with a rapidly increasing number of infected populations worldwide, the testing of each individual was impractical. The wastewater-based epidemiology (WBE) has been implemented to evaluate disease outbreaks as an early warning system for pandemic preparedness. Numerous studies reported the presence of SARS-CoV-2 in the open drains and STPs across the globe via recovery efficiency of surrogate virus from existing virus concentration protocols. However, the such reported studies did not justify the use of WBE to identify or pinpoint the specific hotspots of transmission which could be prioritized for rapid efforts to contain or accelerate active vaccination efforts. Identifying precise locations of hotspots could be an essential aspect in controlling the outbreak and surge of wave by prioritizing the region for primary outbreak response. This article focuses on the issues relating to the primary focus for WBE that can be adapted, and its suitability for utilization in the mass vaccination program is discussed. Effective use of WBE information in terms of source tracking might be crucial as we move towards mass vaccination to control outbreaks of COVID-19 pandemic.10aEpidemiology10aPolicy making10aSARS-CoV-210aVaccination10awastewater1 aDharmadhikari, Tanmay1 aYadav, Rakeshkumar1 aDastager, Syed1 aDharne, Mahesh uhttps://library.ncl.res.in/content/translating-sars-cov-2-wastewater-based-epidemiology-prioritizing-mass-vaccination-strategic02298nas a2200265 4500008004100000022001400041245012000055210006900175260000800244300000800252490000700260520143500267653001201702653002401714653002001738653001701758653001701775100001801792700001901810700002301829700001901852700002201871700001901893856012001912 2022 eng d a2190-572X00aMetagenomic mining of Indian river confluence reveal functional microbial community with lignocelluloytic potential0 aMetagenomic mining of Indian river confluence reveal functional  cJUN a1320 v123 a

Microbial carbohydrate-active enzymes (CAZyme) can be harnessed for valorization of Lignocellulosic biomass (LCB) to value-added chemicals/products. The two Indian Rivers Ganges and the Yamuna having different origins and flow, face accumulation of carbon-rich substrates due to the discharge of wastewater from adjoining paper and pulp industries, which could potentially contribute to the natural enrichment of LCB utilizing genes, especially at their confluence. We analyzed CAZyme diversity in metagenomic datasets across the sacred confluence of the Rivers Ganges and Yamuna. Functional annotation using CAZyme database identified a total of 77,815 putative genes with functional domains involved in the catalysis of carbohydrate degradation or synthesis of glycosidic bonds. The metagenomic analysis detected similar to 41% CAZymes catalyzing the hydrolysis of lignocellulosic biomass polymers- cellulose, hemicellulose, lignin, and pectin. The Beta diversity analysis suggested higher CAZyme diversity at downstream region of the river confluence, which could be useful niche for culture-based studies. Taxonomic origin for CAZymes revealed the predominance of bacteria (97%), followed by archaea (1.67%), Eukaryota (0.63%), and viruses (0.7%). Metagenome guided CAZyme diversity of the microflora spanning across the confluence of Ganges-Yamuna River, could be harnessed for biomass and bioenergy applications.

10aCAZymes10aConfluence (Sangam)10aLignocellulosic10aRiver Ganges10aRiver Yamuna1 aRajput, Vinay1 aSamson, Rachel1 aYadav, Rakeshkumar1 aDastager, Syed1 aKhairnar, Krishna1 aDharne, Mahesh uhttps://library.ncl.res.in/content/metagenomic-mining-indian-river-confluence-reveal-functional-microbial-community02549nas a2200241 4500008004100000022001400041245009700055210006900152260000800221300000800229490000700237520175400244100001501998700002102013700001802034700002302052700001902075700002502094700001902119700002502138700002302163856012102186 2023 eng d a0343-865100aBacterial communities and diversity of western ghats soil: a study of a biodiversity hotspot0 aBacterial communities and diversity of western ghats soil a stud cAPR a1080 v803 a

The Western Ghats is one of India's mega-diversity hotspots and an ecologically and geologically important area for the diversity of endemic plants and animals. The present study provides insights into the aerobic bacterial diversity and composition of the soils of North Western Ghats located in Maharashtra state (NWGM), India. The samples for the culture-dependent study were collected from 6 different locations namely Malshej Ghat, Bhimashankar, Lonavala, Mulshi, Tail-Baila, and Mahabaleshwar. A total of 173 isolates were obtained from the different samples, which belonged to Proteobacteria (43%), Firmicutes (36%), and Actinobacteria (19%). Sequences of 15 strains shared <= 98.7% similarity (a species cut-off) which represent potential novel species. Metagenomic analysis revealed the presence of Actinobacteria and Proteobacteria as the most dominant phyla at both MB and MG. However, both sites showed variation in the composition of rare phyla and other dominant phyla. This difference in bacterial community composition could be due to differences in altitude or other physicochemical properties. The functional prediction from the amplicon sequencing showed the abundance of carbohydrate, protein, and lipid metabolism which was corroborated by screening the isolated bacterial strains for the same. The present study has a unique take on microbial diversity and defines the importance of community assembly processes such as drift, dispersal, and selection. Such processes are relatively important in controlling community diversity, distribution, as well as succession. This study has shown that the microbial community of NWGM is a rich source of polysaccharide degrading bacteria having biotechnological potential.

1 aGhare, Uma1 aNarvekar, Simran1 aLodha, Tushar1 aMallebhari, Rubiya1 aDastager, Syed1 aBarvkar, Vitthal, T.1 aDhotre, Dhiraj1 aKarmalkar, Nitin, R.1 aPable, Anupama, A. uhttps://library.ncl.res.in/content/bacterial-communities-and-diversity-western-ghats-soil-study-biodiversity-hotspot03254nas a2200493 4500008004100000022001400041245016200055210006900217260000800286300001800294490000700312520171400319653002802033653001302061653001802074653001002092653003102102653001202133653001802145653001502163653001502178653003402193100001802227700002002245700001802265700002302283700001902306700001902325700002102344700002102365700002102386700001902407700002402426700002502450700001802475700001802493700001902511700001602530700001902546700001902565700002602584700001902610856013102629 2023 eng d a0944-134400aGenomic surveillance reveals early detection and transition of delta to omicron lineages of SARS-CoV-2 variants in wastewater treatment plants of Pune, India0 aGenomic surveillance reveals early detection and transition of d cDEC a118976-1189880 v303 a

The COVID-19 pandemic has emphasized the urgency for rapid public health surveillance methods to detect and monitor the transmission of infectious diseases. The wastewater-based epidemiology (WBE) has emerged as a promising tool for proactive analysis and quantification of infectious pathogens within a population before clinical cases emerge. In the present study, we aimed to assess the trend and dynamics of SARS-CoV-2 variants using a longitudinal approach. Our objective included early detection and monitoring of these variants to enhance our understanding of their prevalence and potential impact. To achieve our goals, we conducted real-time quantitative polymerase chain reaction (RT-qPCR) and Illumina sequencing on 442 wastewater (WW) samples collected from 10 sewage treatment plants (STPs) in Pune city, India, spanning from November 2021 to April 2022. Our comprehensive analysis identified 426 distinct lineages representing 17 highly transmissible variants of SARS-CoV-2. Notably, fragments of Omicron variant were detected in WW samples prior to its first clinical detection in Botswana. Furthermore, we observed highly contagious sub-lineages of the Omicron variant, including BA.1 (similar to 28%), BA.1.X (1.0-72%), BA.2 (1.0-18%), BA.2.X (1.0-97.4%) BA.2.12 (0.8-0.25%), BA.2.38 (0.8-1.0%), BA.2.75 (0.01-0.02%), BA.3 (0.09-6.3%), BA.4 (0.24-0.29%), and XBB (0.01-21.83%), with varying prevalence rates. Overall, the present study demonstrated the practicality of WBE in the early detection of SARS-CoV-2 variants, which could help track future outbreaks of SARS-CoV-2. Such approaches could be implicated in monitoring infectious agents before they appear in clinical cases.

10aBioinformatics pipeline10aCOVID-1910aEarly warning10aIndia10aNext-generation sequencing10aOmicron10aPublic health10aSARS-CoV-210awastewater10aWastewater-based epidemiology1 aRajput, Vinay1 aPramanik, Rinka1 aMalik, Vinita1 aYadav, Rakeshkumar1 aSamson, Rachel1 aKadam, Pradnya1 aBhalerao, Unnati1 aTupekar, Manisha1 aDeshpande, Dipti1 aShah, Priyanki1 aShashidhara, L., S.1 aBoargaonkar, Radhika1 aPatil, Dhawal1 aKale, Saurabh1 aBhalerao, Asim1 aJain, Nidhi1 aKamble, Sanjay1 aDastager, Syed1 aKarmodiya, Krishanpal1 aDharne, Mahesh uhttps://library.ncl.res.in/content/genomic-surveillance-reveals-early-detection-and-transition-delta-omicron-lineages-sars-cov02808nas a2200289 4500008004100000022001400041245014300055210006900198260000800267300001100275490000800286520184800294653002302142653001902165653002402184653001702208653001502225653001702240100001902257700001802276700002302294700001602317700001902333700002202352700001902374856012502393 2023 eng d a0048-969700aSpatio-temporal variation of the microbiome and resistome repertoire along an anthropogenically dynamic segment of the Ganges River, India0 aSpatiotemporal variation of the microbiome and resistome reperto cMAY a1621250 v8723 a

Aquatic ecosystems are regarded as a hub of antibiotic and metal resistance genes. River Ganges is a unique riverine system in India with socio-cultural and economic significance. However, it remains underexplored for its microbiome and associated resistomes along its anthropogenically impacted course. The present study utilized a nanopore sequenc-ing approach to depict the microbial community structure in the sediments of the river Ganges harboring antibiotic and metal resistance genes (A/MRGs) in lower stretches known for anthropogenic impact. Comprehensive microbiome analyses revealed resistance genes against 23 different types of metals and 28 classes of antibiotics. The most dominant ARG category was multidrug resistance, while the most prevalent MRGs conferred resistance against copper and zinc. Seasonal differences dismally affected the microbiota of the Ganges. However, resistance genes for fosmidomycin and tetracycline varied with season ANOVA, p < 0.05. Interestingly, 333 and 334 ARG subtypes were observed at all the locations in pre-monsoon and post-monsoon, respectively. The taxa associated with the dominant ARGs and MRGs were Pseudomonas and Burkholderia, which are important nosocomial pathogens. A substantial phage diversity for pathogenic and putrefying bacteria at all locations attracts attention for its use to tackle the dissemination of antibiotic and metal-resistant bacteria. This study suggests the accumulation of antibiotics and metals as the driving force for the emergence of resistance genes and the affiliated bacteria trafficking them. The present metagenomic as-sessment highlights the need for comprehensive, long-term biological and physicochemical monitoring and mitigation strategies toward the contaminants associated with ARGs and MRGs in this nationally important river.

10aAntibiotics (ARGs)10aBacteriophages10aHeavy metals (MRGs)10aMetagenomics10aMicrobiome10aRiver Ganges1 aSamson, Rachel1 aRajput, Vinay1 aYadav, Rakeshkumar1 aShah, Manan1 aDastager, Syed1 aKhairnar, Krishna1 aDharne, Mahesh uhttps://library.ncl.res.in/content/spatio-temporal-variation-microbiome-and-resistome-repertoire-along-anthropogenically01051nas a2200313 4500008004100000245011900041210006900160260000800229100001800237700001800255700002000273700002100293700001500314700001700329700001900346700002300365700002100388700001900409700002100428700002200449700001900471700001600490700001900506700001900525700002400544700002300568700001900591856012700610 2024 eng d00aEarly detection of KP.2 SARS-CoV-2 variant using wastewater-based genomic surveillance in Pune, Maharashtra, India0 aEarly detection of KP2 SARSCoV2 variant using wastewaterbased ge cJUL1 aRajput, Vinay1 aDas, Rashmita1 aPramanik, Rinka1 aNannaware, Kiran1 aSushma, Y.1 aTaji, Nyabom1 aRajput, Vishal1 aRajkhowa, Riyakshi1 aPacharne, Poonam1 aShah, Priyanki1 aGogate, Niharika1 aSangwar, Poornima1 aBhalerao, Asim1 aJain, Nidhi1 aKamble, Sanjay1 aDastager, Syed1 aShashidhara, L., S.1 aKaryakarte, Rajesh1 aDharne, Mahesh uhttps://library.ncl.res.in/content/early-detection-kp2-sars-cov-2-variant-using-wastewater-based-genomic-surveillance-pune02874nas a2200229 4500008004100000245011900041210006900160260000800229490000700237520206500244653001902309653002302328653001502351653002602366653002302392100001902415700001902434700001902453700002202472700001902494856013102513 2025 eng d00aDeciphering the comprehensive microbiome of glacier-fed Ganges and functional aspects: implications for one health0 aDeciphering the comprehensive microbiome of glacierfed Ganges an cAUG0 v133 a

Glacier-fed rivers are significant ecological components of the river catchments, yet their microbial diversity and the associated antimicrobial potential remain underexplored. The Ganges is a glacier-fed river of immense cultural, religious, and ecological significance that supports over 400 million people downstream, providing essential water for agriculture, industry, and daily use. Despite its importance, the microbial community composition and antimicrobial potential, across its relatively pristine origin, remain largely underexplored. One possible explanation for this could be the lower microbial load in the upstream glacier-fed region, which likely results in a reduced DNA yield, insufficient for whole-metagenome sequencing, in contrast to the more biologically diverse and nutrient-rich lower reaches. In this study, we developed an efficient DNA extraction and amplification method using low-input DNA to sequence the microbiome from sediments of the glacier-fed Ganges River in pre-monsoon and post-monsoon over 2 years. Taxonomic and functional diversity of bacterial and viral (phage) communities were analyzed, together with the seasonal variations in their composition. Significant differences in microbial communities were observed in response to seasonal shifts (P < 0.05). During the dry season, Proteobacteria and Actinobacteria were predominant, while Bacteroidetes and Firmicutes were abundant post-monsoon (P < 0.05). The microbiome harbors potential for the biosynthesis of streptomycin, phenylpropanoid, penicillin, and cephalosporins. Bacteriophages from Podoviridae, Myoviridae, and Siphoviridae showed lytic potential against putrefying and pathogenic bacteria. This first comprehensive study on the glacier-fed Ganges River highlights significant seasonal shifts in microbial diversity. The initial insights into the functional profile of the bacterial and phage diversity offer opportunities to explore various natural compounds and enzymes to tackle antimicrobial resistance under the one-health canopy.

10aBacteriophages10aglacier-fed-Ganges10aMicrobiome10aSecondary metabolites10aspecial properties1 aSamson, Rachel1 aKumar, Shubham1 aDastager, Syed1 aKhairnar, Krishna1 aDharne, Mahesh uhttps://library.ncl.res.in/content/deciphering-comprehensive-microbiome-glacier-fed-ganges-and-functional-aspects-implications02600nas a2200289 4500008004100000245015500041210006900196260000800265300001400273490000600287520161600293100002001909700001801929700001801947700002101965700001701986700001502003700001902018700001902037700002502056700001902081700002002100700001902120700002602139700001902165856012602184 2025 eng d00aLong-term genomic surveillance of SARS-CoV-2 in campus wastewater depicts lineage trends and public health implications during and after omicron waves0 aLongterm genomic surveillance of SARSCoV2 in campus wastewater d cJUN a908–9190 v33 a

SARS-CoV-2 transmission and detection on academic campuses in low- to middle-income countries has not been explored. The present study explored wastewater surveillance of SARS-CoV-2 in a campus setting in Pune, Maharashtra, India, offering insights into variant-specific trends and their correlation with clinical cases over a 2.5 year period from November 2021 to April 2024. We collected 242 wastewater samples from the campus sewershed and processed them to extract RNA and perform RT-qPCR and sequencing, followed by lineage assignment using the LCS tool. Early signals of different SARS-CoV-2 variants, such as BA.2.X, JN.1.X, and KP.2.X, were detected in wastewater prior to its first clinical report in Maharashtra, India. Wastewater viral load strongly correlated with clinical cases during the Omicron phase (ρ = 0.73–0.81) compared to the post-Omicron phase (ρ = −0.06 to 0.31). This study also highlights that alerts and warnings issued on the basis of wastewater viral hikes have proven instrumental in preventing outbreaks of SARS-CoV-2 variants on campus. However, downgrading COVID-19 from pandemic status by the WHO resulted in a subsequent decrease in public vigilance, changing the viral dynamic in the last phase of the study. This study showcases the utility of wastewater surveillance in a campus setting as an early warning system and understands the interplay of public health policy effects in viral dynamics within controlled ecosystems, such as campuses or offices.

1 aPramanik, Rinka1 aRajput, Vinay1 aMalik, Vinita1 aNannaware, Kiran1 aMatra, Sejal1 aJoshi, Sai1 aKumar, Shubham1 aSamson, Rachel1 aYadav, Rakesh, Kumar1 aShah, Priyanki1 aShashidhara, LS1 aDastager, Syed1 aKarmodiya, Krishanpal1 aDharne, Mahesh uhttps://library.ncl.res.in/content/long-term-genomic-surveillance-sars-cov-2-campus-wastewater-depicts-lineage-trends-and02225nas a2200373 4500008004100000022001400041245018900055210006900244260000800313300000600321490000700327520095400334653001601288653004301304653002401347653001501371653003401386100002001420700002101440700001801461700001901479700002301498700002101521700002401542700002501566700001801591700001801609700001901627700001601646700001901662700001901681700001901700856013201719 2025 eng d a1867-033400aMonitoring influenza A (H1N1, H3N2), RSV, and SARS-CoV-2 using wastewater-based epidemiology: A 2-year longitudinal study in an Indian megacity covering omicron and post-omicron phases0 aMonitoring influenza A H1N1 H3N2 RSV and SARSCoV2 using wastewat cMAR a30 v173 a

{The bourgeoning field of wastewater-based epidemiology (WBE) for the surveillance of several respiratory viruses which includes Influenza A, H1N1pdm09, H3N2, respiratory syncytial viruses (RSV), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is of interest for public health concerns. However, there are few long-term monitoring studies globally. In this study, respiratory viruses were detected and quantified from 11 sewer sheds by utilizing reverse transcription-quantitative polymerase chain reaction analysis in Pune city, India, from Jan 2022 to Dec 2023. The RNA fragments of respiratory viruses were detected in sewage samples before clinical cases were reported, underscoring the potential of WBE for early detection and monitoring within the population. The Spearman correlation of wastewater viral copies was positively and significantly correlated with the clinically positive case of H1N1pdm09 (rho = 0.55

10aInfluenza A10aQuantitative reverse transcription-PCR10aRespiratory viruses10aSARS-CoV-210aWastewater-based epidemiology1 aPramanik, Rinka1 aNannaware, Kiran1 aMalik, Vinita1 aShah, Priyanki1 aSangewar, Poornima1 aGogate, Niharika1 aShashidhara, L., S.1 aBoargaonkar, Radhika1 aPatil, Dhawal1 aKale, Saurabh1 aBhalerao, Asim1 aJain, Nidhi1 aKamble, Sanjay1 aDastager, Syed1 aDharne, Mahesh uhttps://library.ncl.res.in/content/monitoring-influenza-h1n1-h3n2-rsv-and-sars-cov-2-using-wastewater-based-epidemiology-2-year