01978nas a2200289   4500008004100000022001400041245011000055210006900165260009500234300001200329490000600341520094400347653001401291653002101305653001601326653001101342653002301353653001801376100002501394700001601419700002601435700002901461700002601490700001701516700002601533856012901559       2007                        eng d  a1550-703300aCdS quantum dots: enzyme mediated in vitro synthesis, characterization and conjugation with plant lectins0 aCdS quantum dots enzyme mediated in vitro synthesis characteriza  a25650 NORTH LEWIS WAY, STEVENSON RANCH, CA 91381-1439 USAbAMER SCIENTIFIC PUBLISHERScDEC  a406-4130 v33 a<p>This paper presents the in vitro synthesis of CdS quantum dot nanoparticles using sulphite reductase and capping peptide in the presence of a co-factor alpha-NADPH, their characterization and conjugation with plant lectins, Jacalin and Chick pea lectin. Incubation of sulphite reductase with aqueous CdCl2, Na2SO3, and capping peptide in the presence of a-NADPH, which acts as a co-factor, resulted in the formation of CdS nanoparticles of dimensions 5-20 nm. These CdS nanoparticles were conjugated with plant lectins by EDAC mediated coupling. These observations are of significance, as it will help in the development of eco-friendly process for the formation of CdS nanoparticles and their application as fluorescent biolabels. Moreover the above studies will also help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.</p>10abio-label10aCdS quantum dots10aconjugation10aEnzyme10ain vitro synthesis10aNanoparticles1 aAnsary, Abu, Ayoobul1 aKumar, Anil1 aKrishnasastry, M., V.1 aAbyaneh, Majid, Kazemian1 aKulkarni, Sulabha, K.1 aAhmad, Absar1 aKhan, Mohammad, Islam  uhttps://library.ncl.res.in/content/cds-quantum-dots-enzyme-mediated-vitro-synthesis-characterization-and-conjugation-plant-002255nas a2200265   4500008004100000022001400041245013400055210006900189260009500258300001200353490000800365520121200373653002101585653002401606653003501630653001901665653002301684100002501707700002401732700002801756700002501784700002601809700003001835856012401865       2011                        eng d  a0003-269700aCarbon nanotube-modified sodium dodecyl sulfate-polyacrylamide gel electrophoresis for molecular weight determination of proteins0 aCarbon nanotubemodified sodium dodecyl sulfatepolyacrylamide gel  a525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAbACADEMIC PRESS INC ELSEVIER SCIENCEcFEB  a230-2350 v4093 a<p>The effect of incorporating carbon nanotubes (CNTs) in the gel matrix on the electrophoretic mobility of proteins based on their molecular weight differences was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). More specifically, a reduction in standard deviation in the molecular weight calibration plots by 55% in the case of multiwalled carbon nanotubes (MWCNTs) and by 34% in the case of single-walled carbon nanotubes (SWCNTs) compared with that of pristine polyacrylamide gels was achieved after incorporating an insignificant amount of functionalized CNTs into the gel matrix. A mechanism based on a more uniform pore size distribution in CNT modified polyacrylamide gel matrix is proposed. Furthermore, the impact of SWCNTs and MWCNTs on the mobility of proteins in different molecular weight regimes at a given acrylamide concentration offers a tunable gel matrix in terms of the selection of molecular weight ranges of proteins. The robustness and excellent reproducibility of the CNT-PAGE protocol are expected to have a significant impact on the molecular weight determination of newly isolated proteins. (C) 2010 Elsevier Inc. All rights reserved.</p>10aCarbon nanotubes10aGel electrophoresis10aMolecular weight determination10aPolyacrylamide10aProtein separation1 aParthasarathy, Meera1 aDebgupta, Joyashish1 aKakade, Bhalchandra, A.1 aAnsary, Abu, Ayoobul1 aKhan, Mohammad, Islam1 aPillai, Vijayamohanan, K.  uhttps://library.ncl.res.in/content/carbon-nanotube-modified-sodium-dodecyl-sulfate-polyacrylamide-gel-electrophoresis-001804nas a2200241   4500008004100000022001400041245016100055210006900216260009200285300001200377490000600389520084800395653002001243653001101263653003201274653001701306653001701323653002201340100002501362700002301387700002501410856012701435       2012                        eng d  a1947-293500aIn vitro enzyme mediated synthesis of metal sulfide nanoparticles: control of particle size of CdS, Ni7S6, PbS, Co3S4 nanoparticles using synthetic peptides0 aIn vitro enzyme mediated synthesis of metal sulfide nanoparticle  a26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USAbAMER SCIENTIFIC PUBLISHERScJAN  a179-1860 v43 a<p>We report the use of enzyme sulfite reductase for the in vitro synthesis of technologically important metal sulfide quantum dots viz. Co3S4, PbS and Ni7S6. Also we report the use of synthetic peptides to control the particle size of the above metal sulfide quantum dots in addition to CdS, synthesized using enzyme. Sulfite reductase when incubated with aqueous CdCl2, or Co(NO3)(3) or NiCl2 or Pb(NO3)(2), and Na2SO3 in the presence of the appropriate capping peptide resulted in the synthesis of particle size controlled CdS, Co3S4, Ni7S6, or PbS quantum dots respectively. These, enzyme mediated synthesized and size controlled metal sulfide quantum dots, were characterized using Absorbance and photoluminescence spectrometry, X-ray diffraction, X-ray photoelectron spectroscopy and High resolution-transmission electron microscopy.</p>10aCapping Peptide10aEnzyme10aMetal Sulfide Nanoparticles10aquantum dots10aSize Control10aSulfite Reductase1 aAnsary, Abu, Ayoobul1 aKhan, Muhammed, I.1 aGaikwad, Sushama, M.  uhttps://library.ncl.res.in/content/vitro-enzyme-mediated-synthesis-metal-sulfide-nanoparticles-control-particle-size-cds-002651nas a2200289   4500008004100000022001400041245007800055210006900133260010600202300001200308490000800320520167200328653001702000653001702017653002302034653001702057653002402074100001502098700001702113700002502130700001702155700002402172700001602196700002102212700002202233856010602255       2016                        eng d  a1386-142500aBiogenic terbium oxide nanoparticles as the vanguard against osteosarcoma0 aBiogenic terbium oxide nanoparticles as the vanguard against ost  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcNOV  a123-1310 v1683 a<p>The synthesis of inner transition metal nanoparticles via an ecofriendly route is quite difficult. This study, for the first time, reports synthesis of terbium oxide nanoparticles using fungus, Fusarium oxysporum. The biocompatible terbium oxide nanoparticles (Tb2O3 NPs) were synthesized by incubating Tb4O7 with the biomass of fungus F. oxysporum. Multiple physical characterization techniques, such as UV-visible and photoluminescence spectroscopy, TEM, SAED, and zeta-potential were used to confirm the synthesis, purity, optical and surface characteristics, crystallinity, size, shape, distribution, and stability of the nanoemulsion of Tb2O3 NPs. The Tb2O3 NPs were found to inhibit the propagation of MG-63 and Saos-2 cell-lines (IC50 value of 0.102 mu g/mL) and remained non-toxic up to a concentration of 0373 gg/mL toward primary osteoblasts. Cell viability decreased in a concentration-dependent manner upon exposure to 10 nmTb(2)O(3) NPs in the concentration range 0.023-0373 mu g/mL Cell toxicity was evaluated by observing changes in cell morphology, cell viability, oxidative stress parameters, and FACS analysis. Morphological examinations of cells revealed cell shrinkage, nuclear condensation, and formation of apoptotic bodies. The level of ROS within the cells an indicator of oxidative stress was significantly increased. The induction of apoptosis at concentrations &lt;= IC50 was corroborated by 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (DNA damage and nuclear fragmentation). Flow-cytometric studies indicated that the response was dose dependent with a threshold effect. (C) 2016 Elsevier B.V. All rights reserved.</p>10aBiosynthesis10aCytotoxicity10afusarium oxysporum10aOsteosarcoma10aTb2O3 nanoparticles1 aIram, Sana1 aKhan, Salman1 aAnsary, Abu, Ayoobul1 aArshad, Mohd1 aSiddiqui, Sahabjada1 aAhmad, Ejaz1 aKhan, Rizwan, H.1 aKhan, Mohd, Sajid  uhttps://library.ncl.res.in/content/biogenic-terbium-oxide-nanoparticles-vanguard-against-osteosarcoma01737nas a2200145   4500008004100000245008600041210006900127260000800196300001400204520119500218100002501413700001701438700002301455856011301478       2017                        eng d00aBiomimetic synthesis of CdSe nanoparticles with potential bioimaging applications0 aBiomimetic synthesis of CdSe nanoparticles with potential bioima  cJUN  a2526-25323 aThis study presents in vitro biomimetic synthesis of CdSe nanoparticles using the enzyme, NADPH dependent-Nitrate reductase purified from fungus, Fusarium oxysporum. The synthesis of CdSe nanoparticles was accomplished in the presence of a synthetic peptide having amino acid sequence (γ-Glu-Cys-Glu-Cys)-Gly, which acted as binding molecule. The nanoparticles were synthesized in the size range of 3.9–9.0 nm was analysed using TEM.  Further characterization of nanoparticles was done using techniques such as UV, PL, XPS, and FTIR. The as-synthesized CdSe nanoparticles were water dispersible containing free reactive amino groups. These CdSe nanoparticles were conjugated with bi-antennary and tri-antennary glycopeptides opening up the possibilities for their applications in bioimaging. These significant observations will help better understand the mechanism of biosynthesis of fluorescent nanoparticles using fungi. These findings promise an alternative strategy for an eco-friendly, economical, and large-scale synthesis of water dispersible CdSe nanoparticles. These CdSe nanoparticles have promising biomedical-imaging applications without a need for further functionalization.1 aAnsary, Abu, Ayoobul1 aUddin, Imran1 aKhan, Muhammed, I.  uhttps://library.ncl.res.in/content/biomimetic-synthesis-cdse-nanoparticles-potential-bioimaging-applications