02045nas a2200193   4500008004100000022001400041245010100055210006900156260000800225300001200233490000800245520137500253100001901628700002201647700001701669700001601686700002001702856012901722       2026                        eng d  a0032-088900aAutoinhibition of plasma membrane H+-ATPase1 regulates systemic herbivore defense in Arabidopsis0 aAutoinhibition of plasma membrane HATPase1 regulates systemic he  cJUL  akiag4610 v2013 a<p>
	Autoinhibited plasma membrane H+-ATPases1 (AHA1) plays a crucial role in wound-induced systemic signaling, yet its role in insect resistance has not been fully explored. Prior research on fungal and plant AHAs has identified an extended carboxy-terminal tail (C-tail), which forms a regulatory apparatus involving an autoinhibitory domain. Here, we show that truncation of the C-tail of AHA1 increases pump activity and reduces the duration of repolarization after wounding. This also led to reduced expression of the jasmonate pathway marker gene JAZ10 in both wounded and systemic leaves. By specifically increasing AHA1 activity in phloem cells, we further highlighted the essential role of sieve tube elements in mediating electrical signaling and defense responses. Metabolomic analysis showed that AHA1 influences a broad spectrum of metabolites, including amino acids, sugars, and organic acids. Notably, organosulfur compounds like glucosinolates were strongly accumulated in aha1-7 mutants but not in C-tail truncated lines. Collectively, our study identifies the autoinhibitory C-tail of AHA1 as a key regulator that regulates proton pump activity to coordinate electrical signaling, hormonal regulation, and insect defense. These results provide a mechanistic framework for engineering AHA activity to strengthen crop resilience against herbivory.</p>
1 aPawar, Shivani1 aDeshpande, Shweta1 aKundu, Anish1 aGiri, Ashok1 aKumari, Archana  uhttps://library.ncl.res.in/content/autoinhibition-plasma-membrane-h-atpase1-regulates-systemic-herbivore-defense-arabidopsis