02436nas a2200241   4500008004100000022001400041245016800055210006900223260000800292300001200300490000700312520154000319653001901859653002201878653001901900653001701919653001401936100003401950700002401984700002802008700002702036856013102063       2026                        eng d  a1060-132500aGreen synthesis of PLGA and fabrication of topotecan and thymoquinone dual anticancer drug loaded PLGA nanoparticles: a controlled release study for cancer therapy0 aGreen synthesis of PLGA and fabrication of topotecan and thymoqu  cMAR  a232-2460 v633 a<p>
	Cancer therapy is often hindered by poor solubility, low bioavailability, drug resistance, and tumor microenvironmental barriers associated with conventional chemotherapeutics. Polymeric nano-drug delivery systems offer a promising strategy to overcome these limitations, particularly for synergistic multi-drug delivery. In this study, a biodegradable and biocompatible PLGA copolymer (70:30, M-w approximate to 14,500) was synthesized by ring-opening polymerization using zinc proline complex as an initiator through a green route. The copolymer's potential for delivering topotecan (TPT), a water-soluble chemotherapeutic, thymoquinone (TQ), a poorly water-soluble chemotherapeutic, and their combination (TPT+TQ) for cancer treatment was investigated. These nanoparticles demonstrateda consistent particle size &lt; 200 nm high encapsulation efficiency along with desirable controlled-release attributes. Moreover, they exhibited specific release characteristics and cytotoxic effects against HeLa cells, achieving an IC50 value of 20.88 M for the combination therapy (TPT+TQ). Additionally, cytocompatibility testing on L929 fibroblasts confirmed over 98% cell viability for blank PLGA nanoparticles. Additionally, confocal imaging studies confirmed efficient cellular uptake and nuclear localization of the nanoparticles. Overall, the PLGA based dual drug loaded nanoparticles presents a promising approach for targeted, synergistic co-delivery, potentially improving efficacy and reducing toxicity in cancer therapy.</p>
10aBiodegradation10adual drug-loading10aPLGA copolymer10athymoquinone10aTopotecan1 aCheekuramelli, Naga, Srilatha1 aMuhammed, Hasin, N.1 aGarnaik, Baijayantimala1 aNair, Kiran, Sukumaran  uhttps://library.ncl.res.in/content/green-synthesis-plga-and-fabrication-topotecan-and-thymoquinone-dual-anticancer-drug-loaded