02287nas a2200289   4500008004100000022001400041245012500055210006900180260000800249300001200257490000700269520132000276653004001596653002701636653002801663653002601691653001001717653001401727653001301741100001901754700001701773700001701790700002201807700001701829700001901846856013201865       2025                        eng d  a0022-152X00aRegio selective synthesis of pyrazole derivatives of 5-chloro-2-methoxy phenyl hydrazide and their biological evaluation0 aRegio selective synthesis of pyrazole derivatives of 5chloro2met  cAPR  a325-3290 v623 a<p>
	Present study involves synthesis of derivatives of (5-chloro-2-methoxyphenyl) (5-alkyl-3-(substituted) (phenyl/alkyl)-1H-pyrazol-1-yl) methanones. Structural elucidation of the synthesized compounds was depicted by the data of 1H and 13C NMR, IR, and Mass spectral analysis. The newly synthesized compounds 1a-1d and 2a-2i were screened in vitro against Mycobacterium tuberculosis H37Ra using an established XRMA protocol. Among the screened compounds, 2d, 2f, and 2h showed good percent inhibition against the active stage of M. tuberculosis H37Ra 80.77, 55.70, and 79.54, respectively, at 30 mu g/mL of inhibitor concentration. Further in secondary screening, compound 2d exhibited significant antitubercular activity on the active stage of M. tuberculosis H37Ra with IC50 of 0.208 mu g/mL. The synthesized compounds were also screened for antibacterial activity and found no significant activity against Gram-positive Bacteria Bacillus subtitles and Staphylococcus aureus and Gram negative bacteria Pseudomonas aeruginosa and Escherichia coli at 30 mu g/mL, which confirms the specificity of inhibitory activity against M. tuberculosis and more selectively against the active stage. The present study will be helpful for the further development of these molecules into antitubercular lead candidates.</p>
10a5-chloro-2-methoxy phenyl hydrazide10aantibacterial activity10aAntitubercular activity10aBiological evaluation10aH37Ra10ahydrazide10aPyrazole1 aBavishi, Abhay1 aVala, Hardev1 aSwami, Sagar1 aThakrar, Shailesh1 aShah, Anamik1 aSarkar, Dhiman  uhttps://library.ncl.res.in/content/regio-selective-synthesis-pyrazole-derivatives-5-chloro-2-methoxy-phenyl-hydrazide-and-their